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[Refined mapping of the loss of heterozygosity on chromosome 1q21 in gastric carcinoma].
Nan Fang Yi Ke Da Xue Xue Bao. 2008 Jul; 28(7):1229-32.NF

Abstract

OBJECTIVE

To obtain allelic loss mapping and define the minimal lost region on chromosome 1q21 in gastric carcinomas, and explore role of 1q21 loss of heterozygosity (LOH) in the development and progression of gastric carcinogenesis.

METHODS

Using 7 high-density microsatellite markers and PCR method, lq21 LOH was analyzed in 30 paired specimens of fresh gastric carcinoma, and the relation between 1q21 LOH and the clinicopathological features of the malignancy was tested.

RESULTS

The LOH frequency on chromosome 1q21 from these gastric carcinoma tissues reached 60% (18/30). The LOH frequencies of the microsatellite markers D1S514, D1S2696, D1S498, D1S305, D1S2624, D1S2635 and D1S2702 were 13.3%, 10%, 20%, 23.3%, 33.3%, 40% and 23.3%, respectively. The minimal lost region on 1q21 LOH in the gastric carcinoma tissues was located in the region between D1S2624 and D1S2707, in the vicinity of D1S2635. No significant relations of 1q21 LOH to the patients' age, gender, location of the primary foci, clinical staging, or the tumor differentiation were noted (P>0.05), but 1q21 LOH was correlated to lymph node metastases of the malignancy (P<0.05).

CONCLUSION

Higher frequency of 1q21 LOH occurs in gastric carcinoma cells, suggesting the presence of potential tumor suppressor genes closely associated with gastric carcinogenesis near the region of D1S2635.

Authors+Show Affiliations

Cancer Institute, Southern Medical University, Guangzhou 510515, China. liutengfei_1968@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

18676271

Citation

Liu, Teng-fei, et al. "[Refined Mapping of the Loss of Heterozygosity On Chromosome 1q21 in Gastric Carcinoma]." Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, vol. 28, no. 7, 2008, pp. 1229-32.
Liu TF, Yin ZH, Gan RL, et al. [Refined mapping of the loss of heterozygosity on chromosome 1q21 in gastric carcinoma]. Nan Fang Yi Ke Da Xue Xue Bao. 2008;28(7):1229-32.
Liu, T. F., Yin, Z. H., Gan, R. L., Zhao, Q., Dong, L., Zhou, J. G., & Dong, B. H. (2008). [Refined mapping of the loss of heterozygosity on chromosome 1q21 in gastric carcinoma]. Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, 28(7), 1229-32.
Liu TF, et al. [Refined Mapping of the Loss of Heterozygosity On Chromosome 1q21 in Gastric Carcinoma]. Nan Fang Yi Ke Da Xue Xue Bao. 2008;28(7):1229-32. PubMed PMID: 18676271.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Refined mapping of the loss of heterozygosity on chromosome 1q21 in gastric carcinoma]. AU - Liu,Teng-fei, AU - Yin,Zhi-hua, AU - Gan,Run-liang, AU - Zhao,Qiang, AU - Dong,Lin, AU - Zhou,Jian-guo, AU - Dong,Bi-hua, PY - 2008/8/5/pubmed PY - 2009/4/14/medline PY - 2008/8/5/entrez SP - 1229 EP - 32 JF - Nan fang yi ke da xue xue bao = Journal of Southern Medical University JO - Nan Fang Yi Ke Da Xue Xue Bao VL - 28 IS - 7 N2 - OBJECTIVE: To obtain allelic loss mapping and define the minimal lost region on chromosome 1q21 in gastric carcinomas, and explore role of 1q21 loss of heterozygosity (LOH) in the development and progression of gastric carcinogenesis. METHODS: Using 7 high-density microsatellite markers and PCR method, lq21 LOH was analyzed in 30 paired specimens of fresh gastric carcinoma, and the relation between 1q21 LOH and the clinicopathological features of the malignancy was tested. RESULTS: The LOH frequency on chromosome 1q21 from these gastric carcinoma tissues reached 60% (18/30). The LOH frequencies of the microsatellite markers D1S514, D1S2696, D1S498, D1S305, D1S2624, D1S2635 and D1S2702 were 13.3%, 10%, 20%, 23.3%, 33.3%, 40% and 23.3%, respectively. The minimal lost region on 1q21 LOH in the gastric carcinoma tissues was located in the region between D1S2624 and D1S2707, in the vicinity of D1S2635. No significant relations of 1q21 LOH to the patients' age, gender, location of the primary foci, clinical staging, or the tumor differentiation were noted (P>0.05), but 1q21 LOH was correlated to lymph node metastases of the malignancy (P<0.05). CONCLUSION: Higher frequency of 1q21 LOH occurs in gastric carcinoma cells, suggesting the presence of potential tumor suppressor genes closely associated with gastric carcinogenesis near the region of D1S2635. SN - 1673-4254 UR - https://www.unboundmedicine.com/medline/citation/18676271/[Refined_mapping_of_the_loss_of_heterozygosity_on_chromosome_1q21_in_gastric_carcinoma]_ L2 - https://medlineplus.gov/stomachcancer.html DB - PRIME DP - Unbound Medicine ER -