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[Application of the in situ hybridization with EWS dual-color break-apart fluorescence probe and anti-CD99 and anti-FLI-1 antibodies in the diagnosis of Ewing's sarcoma/primitive neuroectodermal tumor].
Beijing Da Xue Xue Bao Yi Xue Ban. 2008 Aug 18; 40(4):358-62.BD

Abstract

OBJECTIVE

To investigate the value of using EWS dual-color, break-apart rearrangement probe fluorescence in situ hybridization (FISH) technique, CD99 and FLI-1 antibodies immunohistochemistry in the diagnosis of Ewing's sarcoma/primitive neuroectodermal tumor (EWS/PNET).

METHODS

Thirty-five cases of EWS/PNET and 24 cases of non-EWS/PNET small round cell tumor were analyzed by FISH and immunohistochemically detected with FLI-1 and CD99 antibodies. Comparison between FISH and immunohistochemical results was carried out and their diagnostic value was evaluated.

RESULTS

The sensitivity, specificity, positive predictive value and negative predictive value of FISH in EWS/PNET were 93.8%(30/32), 81.8%(18/22), 88.2%(30/34) and 90%(18/20); those of CD99 were 100%(35/35), 58.3% (14/24), 77.8%(35/45) and 100%(14/14); those of FLI-1 were 71.4%(25/35), 62.5%(15/24), 73.5%(25/34), 60%(15/25), respectively. The sensitivity, specificity, positive predictive value and negative predictive value of combined use of CD99 and FLI-1 were 71.4%(25/35), 75%(18/24), 80.6%(25/31) and 64.3%(18/28), those of combined use of FLI-1 and FISH were 68.7%(22/32), 86.4%(19/22), 88%(22/25) and 65.5%(19/29), those of combined use of CD99 and FISH were 93.7%(30/32), 95.5%(21/22), 96.8%(30/31) and 91.3%(21/23), respectively.

CONCLUSION

EWS dual-color, break-apart rearrangement probe FISH is a highly sensitive and specific technique in the diagnosis of EWS/PNET. The combination of CD99 and FISH is the method of choice for the diagnosis of EWS/PNET. The combination of CD99 and FLI-1 can improve the specificity in EWS/PNET without the data of FISH.

Authors+Show Affiliations

Department of Pathology, Peking University School of Basic Medical Sciences, Beijing, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

18677380

Citation

Liu, Bao-yue, et al. "[Application of the in Situ Hybridization With EWS Dual-color Break-apart Fluorescence Probe and anti-CD99 and anti-FLI-1 Antibodies in the Diagnosis of Ewing's Sarcoma/primitive Neuroectodermal Tumor]." Beijing Da Xue Xue Bao. Yi Xue Ban = Journal of Peking University. Health Sciences, vol. 40, no. 4, 2008, pp. 358-62.
Liu BY, Yang Y, DU J, et al. [Application of the in situ hybridization with EWS dual-color break-apart fluorescence probe and anti-CD99 and anti-FLI-1 antibodies in the diagnosis of Ewing's sarcoma/primitive neuroectodermal tumor]. Beijing Da Xue Xue Bao Yi Xue Ban. 2008;40(4):358-62.
Liu, B. Y., Yang, Y., DU, J., Zhang, Y., Wang, H., & Zheng, J. (2008). [Application of the in situ hybridization with EWS dual-color break-apart fluorescence probe and anti-CD99 and anti-FLI-1 antibodies in the diagnosis of Ewing's sarcoma/primitive neuroectodermal tumor]. Beijing Da Xue Xue Bao. Yi Xue Ban = Journal of Peking University. Health Sciences, 40(4), 358-62.
Liu BY, et al. [Application of the in Situ Hybridization With EWS Dual-color Break-apart Fluorescence Probe and anti-CD99 and anti-FLI-1 Antibodies in the Diagnosis of Ewing's Sarcoma/primitive Neuroectodermal Tumor]. Beijing Da Xue Xue Bao Yi Xue Ban. 2008 Aug 18;40(4):358-62. PubMed PMID: 18677380.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Application of the in situ hybridization with EWS dual-color break-apart fluorescence probe and anti-CD99 and anti-FLI-1 antibodies in the diagnosis of Ewing's sarcoma/primitive neuroectodermal tumor]. AU - Liu,Bao-yue, AU - Yang,Yu, AU - DU,Juan, AU - Zhang,Yan, AU - Wang,Hua, AU - Zheng,Jie, PY - 2008/8/5/pubmed PY - 2010/6/16/medline PY - 2008/8/5/entrez SP - 358 EP - 62 JF - Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences JO - Beijing Da Xue Xue Bao Yi Xue Ban VL - 40 IS - 4 N2 - OBJECTIVE: To investigate the value of using EWS dual-color, break-apart rearrangement probe fluorescence in situ hybridization (FISH) technique, CD99 and FLI-1 antibodies immunohistochemistry in the diagnosis of Ewing's sarcoma/primitive neuroectodermal tumor (EWS/PNET). METHODS: Thirty-five cases of EWS/PNET and 24 cases of non-EWS/PNET small round cell tumor were analyzed by FISH and immunohistochemically detected with FLI-1 and CD99 antibodies. Comparison between FISH and immunohistochemical results was carried out and their diagnostic value was evaluated. RESULTS: The sensitivity, specificity, positive predictive value and negative predictive value of FISH in EWS/PNET were 93.8%(30/32), 81.8%(18/22), 88.2%(30/34) and 90%(18/20); those of CD99 were 100%(35/35), 58.3% (14/24), 77.8%(35/45) and 100%(14/14); those of FLI-1 were 71.4%(25/35), 62.5%(15/24), 73.5%(25/34), 60%(15/25), respectively. The sensitivity, specificity, positive predictive value and negative predictive value of combined use of CD99 and FLI-1 were 71.4%(25/35), 75%(18/24), 80.6%(25/31) and 64.3%(18/28), those of combined use of FLI-1 and FISH were 68.7%(22/32), 86.4%(19/22), 88%(22/25) and 65.5%(19/29), those of combined use of CD99 and FISH were 93.7%(30/32), 95.5%(21/22), 96.8%(30/31) and 91.3%(21/23), respectively. CONCLUSION: EWS dual-color, break-apart rearrangement probe FISH is a highly sensitive and specific technique in the diagnosis of EWS/PNET. The combination of CD99 and FISH is the method of choice for the diagnosis of EWS/PNET. The combination of CD99 and FLI-1 can improve the specificity in EWS/PNET without the data of FISH. SN - 1671-167X UR - https://www.unboundmedicine.com/medline/citation/18677380/[Application_of_the_in_situ_hybridization_with_EWS_dual_color_break_apart_fluorescence_probe_and_anti_CD99_and_anti_FLI_1_antibodies_in_the_diagnosis_of_Ewing's_sarcoma/primitive_neuroectodermal_tumor]_ L2 - http://xuebao.bjmu.edu.cn/EN/Y2008/V40/I4/358 DB - PRIME DP - Unbound Medicine ER -