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Different effects of spinally applied prostaglandin D2 on responses of dorsal horn neurons with knee input in normal rats and in rats with acute knee inflammation.
Neuroscience. 2008 Sep 22; 156(1):184-92.N

Abstract

Prostaglandin D2(PGD2) is the most produced prostanoid in the CNS of mammals, and in behavioral experiments it has been implicated in the modulation of spinal nociception. In the present study we addressed the effects of spinal PGD2 on the discharge properties of nociceptive spinal cord neurons with input from the knee joint using extracellular recordings in vivo, both in normal rats and in rats with acute inflammation in the knee joint. Topical application of PGD2 to the spinal cord of normal rats did not influence responses to mechanical stimulation of the knee and ankle joint except at a high dose. Specific agonists at either the prostaglandin D2 receptor 1 (DP1) or the prostaglandin D2 receptor 2 (DP2) receptor had no effect on responses to mechanical stimulation of the normal knee. By contrast, in rats with inflamed knee joints either PGD2 or a DP1 receptor agonist decreased responses to mechanical stimulation of the inflamed knee and the non-inflamed ankle thus reducing established inflammation-evoked spinal hyperexcitability. Vice versa, spinal application of an antagonist at DP1 receptors increased responses to mechanical stimulation of the inflamed knee joint and the non-inflamed ankle joint suggesting that endogenous PGD2 attenuated central sensitization under inflammatory conditions, through activation of DP1 receptors. Spinal application of a DP2 receptor antagonist had no effect. The conclusion that spinal PGD2 attenuates spinal hyperexcitability under inflammatory conditions is further supported by the finding that spinal coapplication of PGD2 with prostaglandin E2 (PGE2) attenuated the PGE2-induced facilitation of responses to mechanical stimulation of the normal joint.

Authors+Show Affiliations

Department of Physiology I, Neurophysiology, Friedrich-Schiller-Universität Jena, Teichgraben 8, D-07740 Jena, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18678231

Citation

Telleria-Diaz, A, et al. "Different Effects of Spinally Applied Prostaglandin D2 On Responses of Dorsal Horn Neurons With Knee Input in Normal Rats and in Rats With Acute Knee Inflammation." Neuroscience, vol. 156, no. 1, 2008, pp. 184-92.
Telleria-Diaz A, Ebersberger A, Vasquez E, et al. Different effects of spinally applied prostaglandin D2 on responses of dorsal horn neurons with knee input in normal rats and in rats with acute knee inflammation. Neuroscience. 2008;156(1):184-92.
Telleria-Diaz, A., Ebersberger, A., Vasquez, E., Schache, F., Kahlenbach, J., & Schaible, H. G. (2008). Different effects of spinally applied prostaglandin D2 on responses of dorsal horn neurons with knee input in normal rats and in rats with acute knee inflammation. Neuroscience, 156(1), 184-92. https://doi.org/10.1016/j.neuroscience.2008.07.017
Telleria-Diaz A, et al. Different Effects of Spinally Applied Prostaglandin D2 On Responses of Dorsal Horn Neurons With Knee Input in Normal Rats and in Rats With Acute Knee Inflammation. Neuroscience. 2008 Sep 22;156(1):184-92. PubMed PMID: 18678231.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Different effects of spinally applied prostaglandin D2 on responses of dorsal horn neurons with knee input in normal rats and in rats with acute knee inflammation. AU - Telleria-Diaz,A, AU - Ebersberger,A, AU - Vasquez,E, AU - Schache,F, AU - Kahlenbach,J, AU - Schaible,H-G, Y1 - 2008/07/12/ PY - 2008/04/18/received PY - 2008/06/19/revised PY - 2008/07/10/accepted PY - 2008/8/6/pubmed PY - 2009/1/6/medline PY - 2008/8/6/entrez SP - 184 EP - 92 JF - Neuroscience JO - Neuroscience VL - 156 IS - 1 N2 - Prostaglandin D2(PGD2) is the most produced prostanoid in the CNS of mammals, and in behavioral experiments it has been implicated in the modulation of spinal nociception. In the present study we addressed the effects of spinal PGD2 on the discharge properties of nociceptive spinal cord neurons with input from the knee joint using extracellular recordings in vivo, both in normal rats and in rats with acute inflammation in the knee joint. Topical application of PGD2 to the spinal cord of normal rats did not influence responses to mechanical stimulation of the knee and ankle joint except at a high dose. Specific agonists at either the prostaglandin D2 receptor 1 (DP1) or the prostaglandin D2 receptor 2 (DP2) receptor had no effect on responses to mechanical stimulation of the normal knee. By contrast, in rats with inflamed knee joints either PGD2 or a DP1 receptor agonist decreased responses to mechanical stimulation of the inflamed knee and the non-inflamed ankle thus reducing established inflammation-evoked spinal hyperexcitability. Vice versa, spinal application of an antagonist at DP1 receptors increased responses to mechanical stimulation of the inflamed knee joint and the non-inflamed ankle joint suggesting that endogenous PGD2 attenuated central sensitization under inflammatory conditions, through activation of DP1 receptors. Spinal application of a DP2 receptor antagonist had no effect. The conclusion that spinal PGD2 attenuates spinal hyperexcitability under inflammatory conditions is further supported by the finding that spinal coapplication of PGD2 with prostaglandin E2 (PGE2) attenuated the PGE2-induced facilitation of responses to mechanical stimulation of the normal joint. SN - 0306-4522 UR - https://www.unboundmedicine.com/medline/citation/18678231/Different_effects_of_spinally_applied_prostaglandin_D2_on_responses_of_dorsal_horn_neurons_with_knee_input_in_normal_rats_and_in_rats_with_acute_knee_inflammation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(08)01042-7 DB - PRIME DP - Unbound Medicine ER -