Tags

Type your tag names separated by a space and hit enter

Neuroprotective effects of the nonpsychoactive cannabinoid cannabidiol in hypoxic-ischemic newborn piglets.

Abstract

To test the neuroprotective effects of the nonpsychoactive cannabinoid cannabidiol (CBD), piglets received i.v. CBD or vehicle after hypoxia-ischemia (HI: temporary occlusion of both carotid arteries plus hypoxia). Nonhypoxic-ischemic sham-operated piglets remained as controls. Brain damage was studied by near-infrared spectroscopy (NIRS) and amplitude-integrated electroencephalography (aEEG) and by histologic assessment (Nissl and FluoroJadeB staining). In HI+vehicle, HI led to severe cerebral hemodynamic and metabolic impairment, as reflected in NIRS by an increase in total Hb index (THI) and a decrease in the fractional tissue oxygenation extraction (FTOE); in HI+CBD the increase of THI was blunted and FTOE remained similar to SHAM. HI profoundly decreased EEG amplitude, which was not recovered in HI+vehicle, indicating cerebral hypofunction; seizures were observed in all HI+vehicle. In HI+CBD, however, EEG amplitude recovered to 46.4 7.8% baseline and seizures appeared only in 4/8 piglets (both p < 0.05). The number of viable neurons decreased and that of degenerating neurons increased in HI+vehicle; CBD reduced both effects by more than 50%. CBD administration was free from side effects; moreover, CBD administration was associated with cardiac, hemodynamic, and ventilatory beneficial effects. In conclusion, administration of CBD after HI reduced short-term brain damage and was associated with extracerebral benefits.

Links

  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Laboratorio de Fisiopatología Perinatal Experimental, Gurutzetako Ospitalea, Bilbo, Bizkaia, Spain.

    , , , , , , , ,

    Source

    Pediatric research 64:6 2008 Dec pg 653-8

    MeSH

    Animals
    Animals, Newborn
    Brain
    Cannabidiol
    Electroencephalography
    Hemodynamics
    Hypoxia-Ischemia, Brain
    Neuroprotective Agents
    Swine

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    18679164

    Citation

    Alvarez, Francisco J., et al. "Neuroprotective Effects of the Nonpsychoactive Cannabinoid Cannabidiol in Hypoxic-ischemic Newborn Piglets." Pediatric Research, vol. 64, no. 6, 2008, pp. 653-8.
    Alvarez FJ, Lafuente H, Rey-Santano MC, et al. Neuroprotective effects of the nonpsychoactive cannabinoid cannabidiol in hypoxic-ischemic newborn piglets. Pediatr Res. 2008;64(6):653-8.
    Alvarez, F. J., Lafuente, H., Rey-Santano, M. C., Mielgo, V. E., Gastiasoro, E., Rueda, M., ... Martínez-Orgado, J. (2008). Neuroprotective effects of the nonpsychoactive cannabinoid cannabidiol in hypoxic-ischemic newborn piglets. Pediatric Research, 64(6), pp. 653-8. doi:10.1203/PDR.0b013e318186e5dd.
    Alvarez FJ, et al. Neuroprotective Effects of the Nonpsychoactive Cannabinoid Cannabidiol in Hypoxic-ischemic Newborn Piglets. Pediatr Res. 2008;64(6):653-8. PubMed PMID: 18679164.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Neuroprotective effects of the nonpsychoactive cannabinoid cannabidiol in hypoxic-ischemic newborn piglets. AU - Alvarez,Francisco J, AU - Lafuente,Hector, AU - Rey-Santano,M Carmen, AU - Mielgo,Victoria E, AU - Gastiasoro,Elena, AU - Rueda,Miguel, AU - Pertwee,Roger G, AU - Castillo,Ana I, AU - Romero,Julián, AU - Martínez-Orgado,José, PY - 2008/8/6/pubmed PY - 2009/1/23/medline PY - 2008/8/6/entrez SP - 653 EP - 8 JF - Pediatric research JO - Pediatr. Res. VL - 64 IS - 6 N2 - To test the neuroprotective effects of the nonpsychoactive cannabinoid cannabidiol (CBD), piglets received i.v. CBD or vehicle after hypoxia-ischemia (HI: temporary occlusion of both carotid arteries plus hypoxia). Nonhypoxic-ischemic sham-operated piglets remained as controls. Brain damage was studied by near-infrared spectroscopy (NIRS) and amplitude-integrated electroencephalography (aEEG) and by histologic assessment (Nissl and FluoroJadeB staining). In HI+vehicle, HI led to severe cerebral hemodynamic and metabolic impairment, as reflected in NIRS by an increase in total Hb index (THI) and a decrease in the fractional tissue oxygenation extraction (FTOE); in HI+CBD the increase of THI was blunted and FTOE remained similar to SHAM. HI profoundly decreased EEG amplitude, which was not recovered in HI+vehicle, indicating cerebral hypofunction; seizures were observed in all HI+vehicle. In HI+CBD, however, EEG amplitude recovered to 46.4 7.8% baseline and seizures appeared only in 4/8 piglets (both p < 0.05). The number of viable neurons decreased and that of degenerating neurons increased in HI+vehicle; CBD reduced both effects by more than 50%. CBD administration was free from side effects; moreover, CBD administration was associated with cardiac, hemodynamic, and ventilatory beneficial effects. In conclusion, administration of CBD after HI reduced short-term brain damage and was associated with extracerebral benefits. SN - 1530-0447 UR - https://www.unboundmedicine.com/medline/citation/18679164/Neuroprotective_effects_of_the_nonpsychoactive_cannabinoid_cannabidiol_in_hypoxic_ischemic_newborn_piglets_ L2 - http://dx.doi.org/10.1203/PDR.0b013e318186e5dd DB - PRIME DP - Unbound Medicine ER -