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Dietary fish oil decreases secretion of T helper (Th) 1-type cytokines by a direct effect on murine splenic T cells but enhances secretion of a Th2-type cytokine by an effect on accessory cells.
Br J Nutr. 2009 Apr; 101(7):1040-6.BJ

Abstract

Dietary fish oil is considered to have anti-inflammatory effects based primarily on its effects on T-cell proliferation and IL-2 secretion. Its effects on the secretion of T helper (Th) 1-type cytokines vary and few studies have examined its effects on the secretion of Th2-type cytokines. In the present study, we examined the effects of dietary fish oil on the secretion of Th1 and Th2-type cytokines by splenocytes and the mechanism by which dietary fish oil affects Th2-type cytokine secretion. Mice were fed diets supplemented with 18 % fish oil (w/w) +2 % maize oil or 20 % maize oil for 6 weeks. Spleen cells, isolated splenic T cells and accessory cells (splenocytes depleted of T cells) were stimulated with anti-CD3/anti-CD28. The secretion of interferon (IFN)-gamma, TNF-alpha, IL-4 and IL-10 was measured by ELISA. Dietary fish oil decreased the secretion of IFN-gamma and TNF-alpha by total splenocytes and isolated T cells. In contrast, dietary fish oil increased the secretion of IL-4 by total splenocytes but had no effect on IL-4 secretion by isolated T cells. When isolated T cells were cultured with CD11b+ cells (mainly macrophages), cells from mice fed the fish oil diet secreted more IL-4 than cells from mice fed the maize oil diet. These results demonstrate that dietary fish oil directs cytokine secretion by splenocytes towards a Th2 phenotype and that the effects of dietary fish oil on the secretion of a Th2-type cytokine are mediated by its effect on CD11b+ accessory cells.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Iceland, Vatnsmyrarvegur 16, 101 Reykjavik, Iceland.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18680632

Citation

Petursdottir, Dagbjort H., and Ingibjorg Hardardottir. "Dietary Fish Oil Decreases Secretion of T Helper (Th) 1-type Cytokines By a Direct Effect On Murine Splenic T Cells but Enhances Secretion of a Th2-type Cytokine By an Effect On Accessory Cells." The British Journal of Nutrition, vol. 101, no. 7, 2009, pp. 1040-6.
Petursdottir DH, Hardardottir I. Dietary fish oil decreases secretion of T helper (Th) 1-type cytokines by a direct effect on murine splenic T cells but enhances secretion of a Th2-type cytokine by an effect on accessory cells. Br J Nutr. 2009;101(7):1040-6.
Petursdottir, D. H., & Hardardottir, I. (2009). Dietary fish oil decreases secretion of T helper (Th) 1-type cytokines by a direct effect on murine splenic T cells but enhances secretion of a Th2-type cytokine by an effect on accessory cells. The British Journal of Nutrition, 101(7), 1040-6. https://doi.org/10.1017/S0007114508048290
Petursdottir DH, Hardardottir I. Dietary Fish Oil Decreases Secretion of T Helper (Th) 1-type Cytokines By a Direct Effect On Murine Splenic T Cells but Enhances Secretion of a Th2-type Cytokine By an Effect On Accessory Cells. Br J Nutr. 2009;101(7):1040-6. PubMed PMID: 18680632.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary fish oil decreases secretion of T helper (Th) 1-type cytokines by a direct effect on murine splenic T cells but enhances secretion of a Th2-type cytokine by an effect on accessory cells. AU - Petursdottir,Dagbjort H, AU - Hardardottir,Ingibjorg, Y1 - 2008/08/05/ PY - 2008/8/6/pubmed PY - 2009/6/20/medline PY - 2008/8/6/entrez SP - 1040 EP - 6 JF - The British journal of nutrition JO - Br J Nutr VL - 101 IS - 7 N2 - Dietary fish oil is considered to have anti-inflammatory effects based primarily on its effects on T-cell proliferation and IL-2 secretion. Its effects on the secretion of T helper (Th) 1-type cytokines vary and few studies have examined its effects on the secretion of Th2-type cytokines. In the present study, we examined the effects of dietary fish oil on the secretion of Th1 and Th2-type cytokines by splenocytes and the mechanism by which dietary fish oil affects Th2-type cytokine secretion. Mice were fed diets supplemented with 18 % fish oil (w/w) +2 % maize oil or 20 % maize oil for 6 weeks. Spleen cells, isolated splenic T cells and accessory cells (splenocytes depleted of T cells) were stimulated with anti-CD3/anti-CD28. The secretion of interferon (IFN)-gamma, TNF-alpha, IL-4 and IL-10 was measured by ELISA. Dietary fish oil decreased the secretion of IFN-gamma and TNF-alpha by total splenocytes and isolated T cells. In contrast, dietary fish oil increased the secretion of IL-4 by total splenocytes but had no effect on IL-4 secretion by isolated T cells. When isolated T cells were cultured with CD11b+ cells (mainly macrophages), cells from mice fed the fish oil diet secreted more IL-4 than cells from mice fed the maize oil diet. These results demonstrate that dietary fish oil directs cytokine secretion by splenocytes towards a Th2 phenotype and that the effects of dietary fish oil on the secretion of a Th2-type cytokine are mediated by its effect on CD11b+ accessory cells. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/18680632/Dietary_fish_oil_decreases_secretion_of_T_helper__Th__1_type_cytokines_by_a_direct_effect_on_murine_splenic_T_cells_but_enhances_secretion_of_a_Th2_type_cytokine_by_an_effect_on_accessory_cells_ L2 - https://www.cambridge.org/core/product/identifier/S0007114508048290/type/journal_article DB - PRIME DP - Unbound Medicine ER -