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Subcellular localization of the voltage-gated potassium channels Kv3.1b and Kv3.3 in the cerebellar dentate nucleus of glutamic acid decarboxylase 67-green fluorescent protein transgenic mice.
Neuroscience. 2008 Sep 09; 155(4):1059-69.N

Abstract

Deep cerebellar dentate nuclei are in a key position to control motor planning as a result of an integration of cerebropontine inputs and hemispheric Purkinje neurons signals, and their influence through synaptic outputs onto extracerebellar hubs. GABAergic dentate neurons exhibit broader action potentials and slower afterhyperpolarization than non-GABAergic (presumably glutamatergic) neurons. Specific potassium channels may be involved in these distinct firing profiles, particularly, Kv3.1 and Kv3.3 subunits which rapidly activate at relatively positive potentials to support the generation of fast action potentials. To investigate the subcellular localization of Kv3.1b and Kv3.3 in GAD- and GAD+ dentate neurons of glutamic acid decarboxylase 67-green fluorescent protein (GAD67-GFP) knock-in mice a preembedding immunocytochemical method for electron microscopy was used. Kv3.1b and Kv3.3 were in membranes of cell somata, dendrites, axons and synaptic terminals of both GAD- and GAD+ dentate neurons. The vast majority of GAD- somatodendritic membrane segments domains labeled for Kv3.1b and Kv3.3 (96.1% and 84.7%, respectively) whereas 56.2% and 69.8% of GAD- axonal membrane segments were immunopositive for these subunits. Furthermore, density of Kv3.1b immunoparticles was much higher in GAD- somatodendritic than axonal domains. As to GAD+ neurons, only 70.6% and 50% of somatodendritic membrane segments, and 53.3% and 59.5% of axonal membranes exhibited Kv3.1b and Kv3.3 labeling, respectively. In contrast to GAD- cells, GAD+ cells exhibited a higher density labeling for both Kv3 subunits at their axonal than at their somatodendritic membranes. Taken together, Kv3.1b and Kv3.3 potassium subunits are expressed in both GAD- and GAD+ cells, albeit at different densities and distribution. They likely contribute to the distinct biophysical properties of both GAD- and GAD+ neurons in the dentate nucleus.

Authors+Show Affiliations

Department of Neurosciences, Basque Country University, E-48080 Bilbao, Vizcaya, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18682278

Citation

Alonso-Espinaco, V, et al. "Subcellular Localization of the Voltage-gated Potassium Channels Kv3.1b and Kv3.3 in the Cerebellar Dentate Nucleus of Glutamic Acid Decarboxylase 67-green Fluorescent Protein Transgenic Mice." Neuroscience, vol. 155, no. 4, 2008, pp. 1059-69.
Alonso-Espinaco V, Elezgarai I, Díez-García J, et al. Subcellular localization of the voltage-gated potassium channels Kv3.1b and Kv3.3 in the cerebellar dentate nucleus of glutamic acid decarboxylase 67-green fluorescent protein transgenic mice. Neuroscience. 2008;155(4):1059-69.
Alonso-Espinaco, V., Elezgarai, I., Díez-García, J., Puente, N., Knöpfel, T., & Grandes, P. (2008). Subcellular localization of the voltage-gated potassium channels Kv3.1b and Kv3.3 in the cerebellar dentate nucleus of glutamic acid decarboxylase 67-green fluorescent protein transgenic mice. Neuroscience, 155(4), 1059-69. https://doi.org/10.1016/j.neuroscience.2008.07.014
Alonso-Espinaco V, et al. Subcellular Localization of the Voltage-gated Potassium Channels Kv3.1b and Kv3.3 in the Cerebellar Dentate Nucleus of Glutamic Acid Decarboxylase 67-green Fluorescent Protein Transgenic Mice. Neuroscience. 2008 Sep 9;155(4):1059-69. PubMed PMID: 18682278.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Subcellular localization of the voltage-gated potassium channels Kv3.1b and Kv3.3 in the cerebellar dentate nucleus of glutamic acid decarboxylase 67-green fluorescent protein transgenic mice. AU - Alonso-Espinaco,V, AU - Elezgarai,I, AU - Díez-García,J, AU - Puente,N, AU - Knöpfel,T, AU - Grandes,P, Y1 - 2008/07/12/ PY - 2008/05/13/received PY - 2008/06/16/revised PY - 2008/07/09/accepted PY - 2008/8/7/pubmed PY - 2009/2/7/medline PY - 2008/8/7/entrez SP - 1059 EP - 69 JF - Neuroscience JO - Neuroscience VL - 155 IS - 4 N2 - Deep cerebellar dentate nuclei are in a key position to control motor planning as a result of an integration of cerebropontine inputs and hemispheric Purkinje neurons signals, and their influence through synaptic outputs onto extracerebellar hubs. GABAergic dentate neurons exhibit broader action potentials and slower afterhyperpolarization than non-GABAergic (presumably glutamatergic) neurons. Specific potassium channels may be involved in these distinct firing profiles, particularly, Kv3.1 and Kv3.3 subunits which rapidly activate at relatively positive potentials to support the generation of fast action potentials. To investigate the subcellular localization of Kv3.1b and Kv3.3 in GAD- and GAD+ dentate neurons of glutamic acid decarboxylase 67-green fluorescent protein (GAD67-GFP) knock-in mice a preembedding immunocytochemical method for electron microscopy was used. Kv3.1b and Kv3.3 were in membranes of cell somata, dendrites, axons and synaptic terminals of both GAD- and GAD+ dentate neurons. The vast majority of GAD- somatodendritic membrane segments domains labeled for Kv3.1b and Kv3.3 (96.1% and 84.7%, respectively) whereas 56.2% and 69.8% of GAD- axonal membrane segments were immunopositive for these subunits. Furthermore, density of Kv3.1b immunoparticles was much higher in GAD- somatodendritic than axonal domains. As to GAD+ neurons, only 70.6% and 50% of somatodendritic membrane segments, and 53.3% and 59.5% of axonal membranes exhibited Kv3.1b and Kv3.3 labeling, respectively. In contrast to GAD- cells, GAD+ cells exhibited a higher density labeling for both Kv3 subunits at their axonal than at their somatodendritic membranes. Taken together, Kv3.1b and Kv3.3 potassium subunits are expressed in both GAD- and GAD+ cells, albeit at different densities and distribution. They likely contribute to the distinct biophysical properties of both GAD- and GAD+ neurons in the dentate nucleus. SN - 0306-4522 UR - https://www.unboundmedicine.com/medline/citation/18682278/Subcellular_localization_of_the_voltage_gated_potassium_channels_Kv3_1b_and_Kv3_3_in_the_cerebellar_dentate_nucleus_of_glutamic_acid_decarboxylase_67_green_fluorescent_protein_transgenic_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(08)01038-5 DB - PRIME DP - Unbound Medicine ER -