TY - JOUR T1 - Separation of proteins by consecutive sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and reversed phase high performance liquid chromatography. AU - Kaplan,B, AU - Pras,M, PY - 1991/3/1/pubmed PY - 1991/3/1/medline PY - 1991/3/1/entrez SP - 86 EP - 9 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 5 IS - 2 N2 - A procedure for the preparative separation of proteins was developed by using consecutively sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (SDS-PAGE) and reversed phase high performance liquid chromatography (HPLC). The proteins were separated by SDS-PAGE and afterwards extracted from the gel. The extracted proteins were separated from SDS and other small molecular weight contaminants on a Fractogel TSK HW-40 (F) column in acidic aqueous acetonitrile. The proteins eluted from the Fractogel column were fractionated by HPLC. The identity and purity of the recovered proteins was confirmed by SDS-PAGE analysis. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/1868264/Separation_of_proteins_by_consecutive_sodium_dodecyl_sulfate__SDS__polyacrylamide_gel_electrophoresis_and_reversed_phase_high_performance_liquid_chromatography_ DB - PRIME DP - Unbound Medicine ER -