Tags

Type your tag names separated by a space and hit enter

Expansion of human cytomegalovirus (HCMV) immediate-early 1-specific CD8+ T cells and control of HCMV replication after allogeneic stem cell transplantation.
J Virol. 2008 Oct; 82(20):10143-52.JV

Abstract

Recovery of human cytomegalovirus (HCMV)-specific T immunity is critical for protection against HCMV disease in the early phase after allogeneic stem cell transplantation (SCT). Using an enzyme-linked immunospot assay with overlapping 15-mer peptides spanning pp65 and immediate-early 1 HCMV proteins, we investigated which HCMV-specific CD8(+) gamma interferon-positive (IFN-gamma(+)) T-cell responses against pp65 and IE-1 were associated with control of HCMV replication in 48 recipients of unmanipulated HLA-matched allografts at 3 months (M3) and 6 months (M6) after SCT and in 23 donors. At M3 after SCT, the magnitude of the pp65-specific IFN-gamma-producing CD8(+) T-cell response was greater in recipients than in donors, regardless of HCMV status. In contrast, expansion of IE-1-specific CD8(+) T cells at M3 was associated with protection against HCMV, and no patient with this expansion had HCMV replication at M3. At M6, the number of HCMV-specific CD8(+) T cells against both pp65 and IE-1 had expanded in all recipients, regardless of their previous levels of HCMV replication. The recipients' HCMV-specific CD8(+) T cells already detectable in related donors were predominantly targeting pp65. In contrast, in 40% of the cases, the HCMV-specific CD8(+) T cells in recipients involved new CD8(+) T-cell specificities undetectable in their related donors and preferentially targeting IE-1. Taken together, these results showed that the delay in reconstituting IE-1-specific CD8(+) T cells is correlated with the lack of protection against HCMV in the first 3 months after SCT. They also show that IE-1 is a major antigenic determinant of the early restoration of protective immunity to HCMV after SCT.

Authors+Show Affiliations

Laboratoire d'Immunologie Cellulaire et Tissulaire, INSERM U543, Hôpital Pitié-Salpêtrière, 83 Bld de l'Hôpital, 75651 Paris Cedex 13, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18684826

Citation

Sacre, Karim, et al. "Expansion of Human Cytomegalovirus (HCMV) Immediate-early 1-specific CD8+ T Cells and Control of HCMV Replication After Allogeneic Stem Cell Transplantation." Journal of Virology, vol. 82, no. 20, 2008, pp. 10143-52.
Sacre K, Nguyen S, Deback C, et al. Expansion of human cytomegalovirus (HCMV) immediate-early 1-specific CD8+ T cells and control of HCMV replication after allogeneic stem cell transplantation. J Virol. 2008;82(20):10143-52.
Sacre, K., Nguyen, S., Deback, C., Carcelain, G., Vernant, J. P., Leblond, V., Autran, B., & Dhedin, N. (2008). Expansion of human cytomegalovirus (HCMV) immediate-early 1-specific CD8+ T cells and control of HCMV replication after allogeneic stem cell transplantation. Journal of Virology, 82(20), 10143-52. https://doi.org/10.1128/JVI.00688-08
Sacre K, et al. Expansion of Human Cytomegalovirus (HCMV) Immediate-early 1-specific CD8+ T Cells and Control of HCMV Replication After Allogeneic Stem Cell Transplantation. J Virol. 2008;82(20):10143-52. PubMed PMID: 18684826.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expansion of human cytomegalovirus (HCMV) immediate-early 1-specific CD8+ T cells and control of HCMV replication after allogeneic stem cell transplantation. AU - Sacre,Karim, AU - Nguyen,Stéphanie, AU - Deback,Claire, AU - Carcelain,Guislaine, AU - Vernant,Jean-Paul, AU - Leblond,Véronique, AU - Autran,Brigitte, AU - Dhedin,Nathalie, Y1 - 2008/08/06/ PY - 2008/8/8/pubmed PY - 2008/10/15/medline PY - 2008/8/8/entrez SP - 10143 EP - 52 JF - Journal of virology JO - J Virol VL - 82 IS - 20 N2 - Recovery of human cytomegalovirus (HCMV)-specific T immunity is critical for protection against HCMV disease in the early phase after allogeneic stem cell transplantation (SCT). Using an enzyme-linked immunospot assay with overlapping 15-mer peptides spanning pp65 and immediate-early 1 HCMV proteins, we investigated which HCMV-specific CD8(+) gamma interferon-positive (IFN-gamma(+)) T-cell responses against pp65 and IE-1 were associated with control of HCMV replication in 48 recipients of unmanipulated HLA-matched allografts at 3 months (M3) and 6 months (M6) after SCT and in 23 donors. At M3 after SCT, the magnitude of the pp65-specific IFN-gamma-producing CD8(+) T-cell response was greater in recipients than in donors, regardless of HCMV status. In contrast, expansion of IE-1-specific CD8(+) T cells at M3 was associated with protection against HCMV, and no patient with this expansion had HCMV replication at M3. At M6, the number of HCMV-specific CD8(+) T cells against both pp65 and IE-1 had expanded in all recipients, regardless of their previous levels of HCMV replication. The recipients' HCMV-specific CD8(+) T cells already detectable in related donors were predominantly targeting pp65. In contrast, in 40% of the cases, the HCMV-specific CD8(+) T cells in recipients involved new CD8(+) T-cell specificities undetectable in their related donors and preferentially targeting IE-1. Taken together, these results showed that the delay in reconstituting IE-1-specific CD8(+) T cells is correlated with the lack of protection against HCMV in the first 3 months after SCT. They also show that IE-1 is a major antigenic determinant of the early restoration of protective immunity to HCMV after SCT. SN - 1098-5514 UR - https://www.unboundmedicine.com/medline/citation/18684826/Expansion_of_human_cytomegalovirus__HCMV__immediate_early_1_specific_CD8+_T_cells_and_control_of_HCMV_replication_after_allogeneic_stem_cell_transplantation_ L2 - http://jvi.asm.org/cgi/pmidlookup?view=long&pmid=18684826 DB - PRIME DP - Unbound Medicine ER -