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Comparison of two commercially available dengue virus (DENV) NS1 capture enzyme-linked immunosorbent assays using a single clinical sample for diagnosis of acute DENV infection.
Clin Vaccine Immunol. 2008 Oct; 15(10):1513-8.CV

Abstract

Dengue virus (DENV) nonstructural protein 1 (NS1) has shown promise as a novel diagnostic marker of acute DENV infection. Current techniques used to diagnose acute DENV infection, including virus isolation and reverse transcription-PCR (RT-PCR), are costly and difficult to perform, while traditional serological assays have low sensitivities during the acute stage of infection. Two commercially available NS1 antigen capture enzyme-linked immunosorbent assays (ELISAs), the Platelia dengue NS1Ag test (Bio-Rad Laboratories, Marnes La Coquette, France) and the Pan-E dengue early ELISA test (Panbio Diagnostics, Brisbane, Australia), were evaluated against a well-characterized panel of 208 real-time RT-PCR- and virus isolation-positive sera, as well as 45 real-time RT-PCR- and serologically negative sera from patients with other acute febrile illnesses. The overall sensitivities were 64.9% (95% confidence interval [CI(95)], 58.2 to 71.1%) for the Panbio test and 83.2% (CI(95), 77.5 to 87.7%) for the Bio-Rad test, with interserotype variation, especially for DENV serotype 4. Predictive models were constructed to identify factors that had a significant influence on a test's outcome with respect to this panel of samples in order to identify the conditions in which the test will be most effective as a diagnostic tool. The immunoglobulin G titer was found to be the only covariate that significantly influenced results in the Bio-Rad test, while serotype and the day postonset were found to significantly influence results in the Panbio test. We concluded that the NS1 capture ELISA is a useful tool that can improve testing algorithms to diagnose DENV infection in single samples from acute and early convalescent cases.

Authors+Show Affiliations

Centers for Disease Control and Prevention, Division of Vector-Borne Infectious Diseases, Dengue Branch, SanJuan, Puerto Rico 00920.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

18685015

Citation

Bessoff, Kovi, et al. "Comparison of Two Commercially Available Dengue Virus (DENV) NS1 Capture Enzyme-linked Immunosorbent Assays Using a Single Clinical Sample for Diagnosis of Acute DENV Infection." Clinical and Vaccine Immunology : CVI, vol. 15, no. 10, 2008, pp. 1513-8.
Bessoff K, Delorey M, Sun W, et al. Comparison of two commercially available dengue virus (DENV) NS1 capture enzyme-linked immunosorbent assays using a single clinical sample for diagnosis of acute DENV infection. Clin Vaccine Immunol. 2008;15(10):1513-8.
Bessoff, K., Delorey, M., Sun, W., & Hunsperger, E. (2008). Comparison of two commercially available dengue virus (DENV) NS1 capture enzyme-linked immunosorbent assays using a single clinical sample for diagnosis of acute DENV infection. Clinical and Vaccine Immunology : CVI, 15(10), 1513-8. https://doi.org/10.1128/CVI.00140-08
Bessoff K, et al. Comparison of Two Commercially Available Dengue Virus (DENV) NS1 Capture Enzyme-linked Immunosorbent Assays Using a Single Clinical Sample for Diagnosis of Acute DENV Infection. Clin Vaccine Immunol. 2008;15(10):1513-8. PubMed PMID: 18685015.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of two commercially available dengue virus (DENV) NS1 capture enzyme-linked immunosorbent assays using a single clinical sample for diagnosis of acute DENV infection. AU - Bessoff,Kovi, AU - Delorey,Mark, AU - Sun,Wellington, AU - Hunsperger,Elizabeth, Y1 - 2008/08/06/ PY - 2008/8/8/pubmed PY - 2008/11/7/medline PY - 2008/8/8/entrez SP - 1513 EP - 8 JF - Clinical and vaccine immunology : CVI JO - Clin Vaccine Immunol VL - 15 IS - 10 N2 - Dengue virus (DENV) nonstructural protein 1 (NS1) has shown promise as a novel diagnostic marker of acute DENV infection. Current techniques used to diagnose acute DENV infection, including virus isolation and reverse transcription-PCR (RT-PCR), are costly and difficult to perform, while traditional serological assays have low sensitivities during the acute stage of infection. Two commercially available NS1 antigen capture enzyme-linked immunosorbent assays (ELISAs), the Platelia dengue NS1Ag test (Bio-Rad Laboratories, Marnes La Coquette, France) and the Pan-E dengue early ELISA test (Panbio Diagnostics, Brisbane, Australia), were evaluated against a well-characterized panel of 208 real-time RT-PCR- and virus isolation-positive sera, as well as 45 real-time RT-PCR- and serologically negative sera from patients with other acute febrile illnesses. The overall sensitivities were 64.9% (95% confidence interval [CI(95)], 58.2 to 71.1%) for the Panbio test and 83.2% (CI(95), 77.5 to 87.7%) for the Bio-Rad test, with interserotype variation, especially for DENV serotype 4. Predictive models were constructed to identify factors that had a significant influence on a test's outcome with respect to this panel of samples in order to identify the conditions in which the test will be most effective as a diagnostic tool. The immunoglobulin G titer was found to be the only covariate that significantly influenced results in the Bio-Rad test, while serotype and the day postonset were found to significantly influence results in the Panbio test. We concluded that the NS1 capture ELISA is a useful tool that can improve testing algorithms to diagnose DENV infection in single samples from acute and early convalescent cases. SN - 1556-679X UR - https://www.unboundmedicine.com/medline/citation/18685015/Comparison_of_two_commercially_available_dengue_virus__DENV__NS1_capture_enzyme_linked_immunosorbent_assays_using_a_single_clinical_sample_for_diagnosis_of_acute_DENV_infection_ L2 - http://cvi.asm.org/cgi/pmidlookup?view=long&pmid=18685015 DB - PRIME DP - Unbound Medicine ER -