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Crystal structure of ST2348, a CBS domain protein, from hyperthermophilic archaeon Sulfolobus tokodaii.
Biochem Biophys Res Commun. 2008 Oct 10; 375(1):124-8.BB

Abstract

The crystal structure of a hypothetical protein ST2348 (GI: 47118305) from the hyperthermophilic bacteria Sulfolobus tokodaii has been determined using X-ray crystallography. The protein consists of two CBS (cystathione beta synthase) domains, whose function has been analyzed and reported here. PSI-BLAST shows a conservation of this domain in about 100 proteins in various species. However, none of the close homologs of ST2348 have been functionally characterized so far. Structure and sequence comparison of ST2348 with human AMP-kinase gamma1 subunit and the CBS domain pair of bacterial IMP dehydrogenase is suggestive of its binding to AMP and ATP. A highly conserved residue Asp118, located in a negatively charged patch near the ligand binding cleft, could serve as a site for phosphorylation similar to that found in the chemotatic signal protein CheY and thereby ST2348 can function as a signal transduction molecule.

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Authors+Show Affiliations

Ragunathan P
Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai 600 025, India.
Kumarevel T
No affiliation info available
Agari Y
No affiliation info available
Shinkai A
No affiliation info available
Kuramitsu S
No affiliation info available
Yokoyama S
No affiliation info available
Ponnuraj K
No affiliation info available

MeSH

Archaeal ProteinsCrystallography, X-RayCystathionine beta-SynthaseHot TemperatureHumansProtein FoldingProtein Structure, TertiarySulfolobus

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18691556

Citation

Ragunathan, Preethi, et al. "Crystal Structure of ST2348, a CBS Domain Protein, From Hyperthermophilic Archaeon Sulfolobus Tokodaii." Biochemical and Biophysical Research Communications, vol. 375, no. 1, 2008, pp. 124-8.
Ragunathan P, Kumarevel T, Agari Y, et al. Crystal structure of ST2348, a CBS domain protein, from hyperthermophilic archaeon Sulfolobus tokodaii. Biochem Biophys Res Commun. 2008;375(1):124-8.
Ragunathan, P., Kumarevel, T., Agari, Y., Shinkai, A., Kuramitsu, S., Yokoyama, S., & Ponnuraj, K. (2008). Crystal structure of ST2348, a CBS domain protein, from hyperthermophilic archaeon Sulfolobus tokodaii. Biochemical and Biophysical Research Communications, 375(1), 124-8. https://doi.org/10.1016/j.bbrc.2008.07.140
Ragunathan P, et al. Crystal Structure of ST2348, a CBS Domain Protein, From Hyperthermophilic Archaeon Sulfolobus Tokodaii. Biochem Biophys Res Commun. 2008 Oct 10;375(1):124-8. PubMed PMID: 18691556.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Crystal structure of ST2348, a CBS domain protein, from hyperthermophilic archaeon Sulfolobus tokodaii. AU - Ragunathan,Preethi, AU - Kumarevel,Thirumananseri, AU - Agari,Yoshihiro, AU - Shinkai,Akeo, AU - Kuramitsu,Seiki, AU - Yokoyama,Shigeyuki, AU - Ponnuraj,Karthe, Y1 - 2008/08/06/ PY - 2008/07/21/received PY - 2008/07/25/accepted PY - 2008/8/12/pubmed PY - 2008/9/10/medline PY - 2008/8/12/entrez SP - 124 EP - 8 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 375 IS - 1 N2 - The crystal structure of a hypothetical protein ST2348 (GI: 47118305) from the hyperthermophilic bacteria Sulfolobus tokodaii has been determined using X-ray crystallography. The protein consists of two CBS (cystathione beta synthase) domains, whose function has been analyzed and reported here. PSI-BLAST shows a conservation of this domain in about 100 proteins in various species. However, none of the close homologs of ST2348 have been functionally characterized so far. Structure and sequence comparison of ST2348 with human AMP-kinase gamma1 subunit and the CBS domain pair of bacterial IMP dehydrogenase is suggestive of its binding to AMP and ATP. A highly conserved residue Asp118, located in a negatively charged patch near the ligand binding cleft, could serve as a site for phosphorylation similar to that found in the chemotatic signal protein CheY and thereby ST2348 can function as a signal transduction molecule. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/18691556/Crystal_structure_of_ST2348_a_CBS_domain_protein_from_hyperthermophilic_archaeon_Sulfolobus_tokodaii_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(08)01482-4 DB - PRIME DP - Unbound Medicine ER -