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Iron oxide nanoparticles-chitosan composite based glucose biosensor.
Biosens Bioelectron. 2008 Dec 01; 24(4):676-83.BB

Abstract

Iron oxide (Fe(3)O(4)) nanoparticles prepared using co-precipitation method have been dispersed in chitosan (CH) solution to fabricate nanocomposite film on indium-tin oxide (ITO) glass plate. Glucose oxidase (GOx) has been immobilized onto this CH-Fe(3)O(4) nanocomposite film via physical adsorption. The size of the Fe(3)O(4) nanoparticles estimated using X-ray diffraction (XRD) pattern and transmission electron microscopy (TEM) has been found to be approximately 22 nm. The CH-Fe(3)O(4) nanocomposite film and GOx/CH-Fe(3)O(4)/ITO bioelectrode have been characterized using UV-visible and Fourier transform infrared (FTIR) spectroscopic and scanning electron microscopy (SEM) techniques, respectively. This GOx/CH-Fe(3)O(4)/ITO nanocomposite bioelectrode has response time of 5s, linearity as 10-400 mgdL(-1) of glucose, sensitivity as 9.3 microA/(mgdLcm(2)) and shelf life of about 8 weeks under refrigerated conditions. The value of Michaelis-Menten (K(m)) constant obtained as 0.141 mM indicates high affinity of immobilized GOx towards the substrate (glucose).

Authors+Show Affiliations

Biomolecular Electronics & Conducting Polymer Research Group, National Physical Laboratory, K.S. Krishnan Marg, New Delhi 110012, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18692384

Citation

Kaushik, Ajeet, et al. "Iron Oxide Nanoparticles-chitosan Composite Based Glucose Biosensor." Biosensors & Bioelectronics, vol. 24, no. 4, 2008, pp. 676-83.
Kaushik A, Khan R, Solanki PR, et al. Iron oxide nanoparticles-chitosan composite based glucose biosensor. Biosens Bioelectron. 2008;24(4):676-83.
Kaushik, A., Khan, R., Solanki, P. R., Pandey, P., Alam, J., Ahmad, S., & Malhotra, B. D. (2008). Iron oxide nanoparticles-chitosan composite based glucose biosensor. Biosensors & Bioelectronics, 24(4), 676-83. https://doi.org/10.1016/j.bios.2008.06.032
Kaushik A, et al. Iron Oxide Nanoparticles-chitosan Composite Based Glucose Biosensor. Biosens Bioelectron. 2008 Dec 1;24(4):676-83. PubMed PMID: 18692384.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Iron oxide nanoparticles-chitosan composite based glucose biosensor. AU - Kaushik,Ajeet, AU - Khan,Raju, AU - Solanki,Pratima R, AU - Pandey,Pratibha, AU - Alam,Javed, AU - Ahmad,Sharif, AU - Malhotra,B D, Y1 - 2008/07/01/ PY - 2008/03/21/received PY - 2008/04/30/revised PY - 2008/06/13/accepted PY - 2008/8/12/pubmed PY - 2009/2/13/medline PY - 2008/8/12/entrez SP - 676 EP - 83 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 24 IS - 4 N2 - Iron oxide (Fe(3)O(4)) nanoparticles prepared using co-precipitation method have been dispersed in chitosan (CH) solution to fabricate nanocomposite film on indium-tin oxide (ITO) glass plate. Glucose oxidase (GOx) has been immobilized onto this CH-Fe(3)O(4) nanocomposite film via physical adsorption. The size of the Fe(3)O(4) nanoparticles estimated using X-ray diffraction (XRD) pattern and transmission electron microscopy (TEM) has been found to be approximately 22 nm. The CH-Fe(3)O(4) nanocomposite film and GOx/CH-Fe(3)O(4)/ITO bioelectrode have been characterized using UV-visible and Fourier transform infrared (FTIR) spectroscopic and scanning electron microscopy (SEM) techniques, respectively. This GOx/CH-Fe(3)O(4)/ITO nanocomposite bioelectrode has response time of 5s, linearity as 10-400 mgdL(-1) of glucose, sensitivity as 9.3 microA/(mgdLcm(2)) and shelf life of about 8 weeks under refrigerated conditions. The value of Michaelis-Menten (K(m)) constant obtained as 0.141 mM indicates high affinity of immobilized GOx towards the substrate (glucose). SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/18692384/Iron_oxide_nanoparticles_chitosan_composite_based_glucose_biosensor_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(08)00279-0 DB - PRIME DP - Unbound Medicine ER -