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Preparative isolation and purification of two new isomeric diterpenoid alkaloids from Aconitum coreanum by high-speed counter-current chromatography.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Sep 01; 872(1-2):181-5.JC

Abstract

Preparative high-speed counter-current chromatography (HSCCC) coupled with evaporative light scattering detection (ELSD) was used to isolate and separate bioactive constituents from the roots of Aconitum coreanum. Two new diterpenoid alkaloid isomers were successfully separated for the first time by HSCCC with an optimized two-phase solvent system composed of ethyl acetate-n-butanol-methanol-2% acetic acid (3.5:1.5:2:4.5, v/v/v/v), 25.4 mg of GFT (1) and 18.3 mg of GFU (2) were isolated form 1 g crude extract in one step HSCCC experiment. The purities of the two new compounds were all over 95% as analyzed by HPLC and their structures were identified by ESI-MS, (1)H NMR, (13)C NMR, and 2D NMR analysis.

Authors+Show Affiliations

Key Laboratory of Research of Traditional Chinese Medicine and New Drug, Southern Medical University, Guangzhou 510515, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18692446

Citation

Tang, Qingfa, et al. "Preparative Isolation and Purification of Two New Isomeric Diterpenoid Alkaloids From Aconitum Coreanum By High-speed Counter-current Chromatography." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 872, no. 1-2, 2008, pp. 181-5.
Tang Q, Liu J, Xue J, et al. Preparative isolation and purification of two new isomeric diterpenoid alkaloids from Aconitum coreanum by high-speed counter-current chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2008;872(1-2):181-5.
Tang, Q., Liu, J., Xue, J., Ye, W., Zhang, Z., & Yang, C. (2008). Preparative isolation and purification of two new isomeric diterpenoid alkaloids from Aconitum coreanum by high-speed counter-current chromatography. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 872(1-2), 181-5. https://doi.org/10.1016/j.jchromb.2008.07.030
Tang Q, et al. Preparative Isolation and Purification of Two New Isomeric Diterpenoid Alkaloids From Aconitum Coreanum By High-speed Counter-current Chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Sep 1;872(1-2):181-5. PubMed PMID: 18692446.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparative isolation and purification of two new isomeric diterpenoid alkaloids from Aconitum coreanum by high-speed counter-current chromatography. AU - Tang,Qingfa, AU - Liu,Jinghan, AU - Xue,Jiao, AU - Ye,Wencai, AU - Zhang,Zunjian, AU - Yang,Chunhua, Y1 - 2008/07/30/ PY - 2008/04/10/received PY - 2008/07/17/revised PY - 2008/07/23/accepted PY - 2008/8/12/pubmed PY - 2008/10/29/medline PY - 2008/8/12/entrez SP - 181 EP - 5 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 872 IS - 1-2 N2 - Preparative high-speed counter-current chromatography (HSCCC) coupled with evaporative light scattering detection (ELSD) was used to isolate and separate bioactive constituents from the roots of Aconitum coreanum. Two new diterpenoid alkaloid isomers were successfully separated for the first time by HSCCC with an optimized two-phase solvent system composed of ethyl acetate-n-butanol-methanol-2% acetic acid (3.5:1.5:2:4.5, v/v/v/v), 25.4 mg of GFT (1) and 18.3 mg of GFU (2) were isolated form 1 g crude extract in one step HSCCC experiment. The purities of the two new compounds were all over 95% as analyzed by HPLC and their structures were identified by ESI-MS, (1)H NMR, (13)C NMR, and 2D NMR analysis. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/18692446/Preparative_isolation_and_purification_of_two_new_isomeric_diterpenoid_alkaloids_from_Aconitum_coreanum_by_high_speed_counter_current_chromatography_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(08)00570-9 DB - PRIME DP - Unbound Medicine ER -