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Differential expression of branchial Na+/K(+)-ATPase of two medaka species, Oryzias latipes and Oryzias dancena, with different salinity tolerances acclimated to fresh water, brackish water and seawater.
Comp Biochem Physiol A Mol Integr Physiol. 2008 Dec; 151(4):566-75.CB

Abstract

Previous studies on non-diadromous euryhaline teleosts introduced a hypothesis that the lowest level of gill Na(+)/K(+)-ATPase (NKA) activity occurs in the environments with salinity close to the primary natural habitats of the studied species. To provide more evidence of the hypothesis, two medaka species, Oryzias latipes and O. dancena, whose primary natural habitats are fresh water (FW) and brackish water (BW) environments, respectively, were compared from levels of mRNA to cells in this study. The plasma osmolalities of O. latipes and O. dancena were lowest in the FW individuals. The muscle water contents of O. latipes decreased with elevated external salinities, but were constant among FW-, BW-, and seawater (SW)-acclimated O. dancena. Expression of NKA, the primary driving force of ion transporters in gill ionocytes, revealed different patterns in the two Oryzias species. The highest NKA alpha-subunit mRNA abundances were found in the gills of the SW O. latipes and the FW O. dancena, respectively. The pattern of NKA activity and alpha-subunit protein abundance in the gills of O. latipes revealed that the FW group was the lowest, while the pattern in O. dancena revealed that the BW group was the lowest. Immunohistochemical staining showed similar profiles of NKA immunoreactive (NKIR) cell activities (NKIR cell numberxcell size) in the gills of these two species among FW, BW, and SW groups. Taken together, O. latipes exhibited better hyposmoregulatory ability, while O. dancena exhibited better hyperosmoregulatory ability. Our results corresponding to the hypothesis indicated that the lowest branchial NKA activities of these two medaka species were found in the environments with salinities similar to their natural habitats.

Authors+Show Affiliations

Department of Life Sciences, National Chung-Hsing University, Taichung 402, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

18692588

Citation

Kang, Chao-Kai, et al. "Differential Expression of Branchial Na+/K(+)-ATPase of Two Medaka Species, Oryzias Latipes and Oryzias Dancena, With Different Salinity Tolerances Acclimated to Fresh Water, Brackish Water and Seawater." Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, vol. 151, no. 4, 2008, pp. 566-75.
Kang CK, Tsai SC, Lee TH, et al. Differential expression of branchial Na+/K(+)-ATPase of two medaka species, Oryzias latipes and Oryzias dancena, with different salinity tolerances acclimated to fresh water, brackish water and seawater. Comp Biochem Physiol A Mol Integr Physiol. 2008;151(4):566-75.
Kang, C. K., Tsai, S. C., Lee, T. H., & Hwang, P. P. (2008). Differential expression of branchial Na+/K(+)-ATPase of two medaka species, Oryzias latipes and Oryzias dancena, with different salinity tolerances acclimated to fresh water, brackish water and seawater. Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 151(4), 566-75. https://doi.org/10.1016/j.cbpa.2008.07.020
Kang CK, et al. Differential Expression of Branchial Na+/K(+)-ATPase of Two Medaka Species, Oryzias Latipes and Oryzias Dancena, With Different Salinity Tolerances Acclimated to Fresh Water, Brackish Water and Seawater. Comp Biochem Physiol A Mol Integr Physiol. 2008;151(4):566-75. PubMed PMID: 18692588.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential expression of branchial Na+/K(+)-ATPase of two medaka species, Oryzias latipes and Oryzias dancena, with different salinity tolerances acclimated to fresh water, brackish water and seawater. AU - Kang,Chao-Kai, AU - Tsai,Shu-Chuan, AU - Lee,Tsung-Han, AU - Hwang,Pung-Pung, Y1 - 2008/07/20/ PY - 2008/04/17/received PY - 2008/07/10/revised PY - 2008/07/10/accepted PY - 2008/8/12/pubmed PY - 2009/12/30/medline PY - 2008/8/12/entrez SP - 566 EP - 75 JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology JO - Comp Biochem Physiol A Mol Integr Physiol VL - 151 IS - 4 N2 - Previous studies on non-diadromous euryhaline teleosts introduced a hypothesis that the lowest level of gill Na(+)/K(+)-ATPase (NKA) activity occurs in the environments with salinity close to the primary natural habitats of the studied species. To provide more evidence of the hypothesis, two medaka species, Oryzias latipes and O. dancena, whose primary natural habitats are fresh water (FW) and brackish water (BW) environments, respectively, were compared from levels of mRNA to cells in this study. The plasma osmolalities of O. latipes and O. dancena were lowest in the FW individuals. The muscle water contents of O. latipes decreased with elevated external salinities, but were constant among FW-, BW-, and seawater (SW)-acclimated O. dancena. Expression of NKA, the primary driving force of ion transporters in gill ionocytes, revealed different patterns in the two Oryzias species. The highest NKA alpha-subunit mRNA abundances were found in the gills of the SW O. latipes and the FW O. dancena, respectively. The pattern of NKA activity and alpha-subunit protein abundance in the gills of O. latipes revealed that the FW group was the lowest, while the pattern in O. dancena revealed that the BW group was the lowest. Immunohistochemical staining showed similar profiles of NKA immunoreactive (NKIR) cell activities (NKIR cell numberxcell size) in the gills of these two species among FW, BW, and SW groups. Taken together, O. latipes exhibited better hyposmoregulatory ability, while O. dancena exhibited better hyperosmoregulatory ability. Our results corresponding to the hypothesis indicated that the lowest branchial NKA activities of these two medaka species were found in the environments with salinities similar to their natural habitats. SN - 1531-4332 UR - https://www.unboundmedicine.com/medline/citation/18692588/Differential_expression_of_branchial_Na+/K_+__ATPase_of_two_medaka_species_Oryzias_latipes_and_Oryzias_dancena_with_different_salinity_tolerances_acclimated_to_fresh_water_brackish_water_and_seawater_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1095-6433(08)01047-7 DB - PRIME DP - Unbound Medicine ER -