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Analysis of CRE-mediated recombination driven by myosin light chain 1/3 regulatory elements in embryonic and adult skeletal muscle: a tool to study fiber specification.
Genesis. 2008 Aug; 46(8):424-30.G

Abstract

An increasing number of genes have been implicated in skeletal muscle fiber diversity. To study the contribution of diverse genetic elements to the regulation of fiber-type composition, we generated a transgenic mouse in which CRE recombinase expression is driven by muscle-specific regulatory sequences of the myosin light chain 1/3 locus (MLC). Using ROSA26 conditional reporter mice, we detected expression of the MLC-Cre transgene starting from embryonic day 12.5 (E12.5). By E15, recombination was detected in all muscle-derived structures. Immunohistochemical analysis revealed CRE activity was restricted to fast-twitch (type II) and excluded from slow-twitch (type I) fibers of skeletal muscle. The MLC-Cre transgenic mouse can be used in conjunction with conditional alleles to study both developmental patterning and maintenance of fast fiber-type phenotypes.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Letter
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18693277

Citation

Mourkioti, Foteini, et al. "Analysis of CRE-mediated Recombination Driven By Myosin Light Chain 1/3 Regulatory Elements in Embryonic and Adult Skeletal Muscle: a Tool to Study Fiber Specification." Genesis (New York, N.Y. : 2000), vol. 46, no. 8, 2008, pp. 424-30.
Mourkioti F, Slonimsky E, Huth M, et al. Analysis of CRE-mediated recombination driven by myosin light chain 1/3 regulatory elements in embryonic and adult skeletal muscle: a tool to study fiber specification. Genesis. 2008;46(8):424-30.
Mourkioti, F., Slonimsky, E., Huth, M., Berno, V., & Rosenthal, N. (2008). Analysis of CRE-mediated recombination driven by myosin light chain 1/3 regulatory elements in embryonic and adult skeletal muscle: a tool to study fiber specification. Genesis (New York, N.Y. : 2000), 46(8), 424-30. https://doi.org/10.1002/dvg.20419
Mourkioti F, et al. Analysis of CRE-mediated Recombination Driven By Myosin Light Chain 1/3 Regulatory Elements in Embryonic and Adult Skeletal Muscle: a Tool to Study Fiber Specification. Genesis. 2008;46(8):424-30. PubMed PMID: 18693277.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of CRE-mediated recombination driven by myosin light chain 1/3 regulatory elements in embryonic and adult skeletal muscle: a tool to study fiber specification. AU - Mourkioti,Foteini, AU - Slonimsky,Esfir, AU - Huth,Marion, AU - Berno,Valeria, AU - Rosenthal,Nadia, PY - 2008/8/12/pubmed PY - 2008/9/23/medline PY - 2008/8/12/entrez SP - 424 EP - 30 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 46 IS - 8 N2 - An increasing number of genes have been implicated in skeletal muscle fiber diversity. To study the contribution of diverse genetic elements to the regulation of fiber-type composition, we generated a transgenic mouse in which CRE recombinase expression is driven by muscle-specific regulatory sequences of the myosin light chain 1/3 locus (MLC). Using ROSA26 conditional reporter mice, we detected expression of the MLC-Cre transgene starting from embryonic day 12.5 (E12.5). By E15, recombination was detected in all muscle-derived structures. Immunohistochemical analysis revealed CRE activity was restricted to fast-twitch (type II) and excluded from slow-twitch (type I) fibers of skeletal muscle. The MLC-Cre transgenic mouse can be used in conjunction with conditional alleles to study both developmental patterning and maintenance of fast fiber-type phenotypes. SN - 1526-968X UR - https://www.unboundmedicine.com/medline/citation/18693277/Analysis_of_CRE_mediated_recombination_driven_by_myosin_light_chain_1/3_regulatory_elements_in_embryonic_and_adult_skeletal_muscle:_a_tool_to_study_fiber_specification_ L2 - https://doi.org/10.1002/dvg.20419 DB - PRIME DP - Unbound Medicine ER -