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Molecular and phenotypic characterization of near isogenic lines at QTL for quantitative resistance to Leptosphaeria maculans in oilseed rape (Brassica napus L.).
Theor Appl Genet. 2008 Nov; 117(7):1055-67.TA

Abstract

The most common and effective way to control phoma stem canker (blackleg) caused by Leptosphaeria maculans in oilseed rape (Brassica napus) is by breeding resistant cultivars. Specific resistance genes have been identified in B. napus and related species but in some B. napus cultivars resistance is polygenic [mediated by quantitative trait loci (QTL)], postulated to be race non-specific and durable. The genetic basis of quantitative resistance in the French winter oilseed rape 'Darmor', which was derived from 'Jet Neuf', was previously examined in two genetic backgrounds. Stable QTL involved in blackleg resistance across year and genetic backgrounds were identified. In this study, near isogenic lines (NILs) were produced in the susceptible background 'Yudal' for four of these QTL using marker-assisted selection. Various strategies were used to develop new molecular markers, which were mapped in these QTL regions. These were used to characterize the length and homozygosity of the 'Darmor-bzh' introgressed segment in the NILs. Individuals from each NIL were evaluated in blackleg disease field trials and assessed for their level of stem canker in comparison to the recurrent line 'Yudal'. The effect of QTL LmA2 was clearly validated and to a lesser extent, QTL LmA9 also showed an effect on the disease level. This work provides valuable material that can be used to study the mode of action of genetic factors involved in L. maculans quantitative resistance.

Authors+Show Affiliations

UMR 118 Amélioration des Plantes et Biotechnologies Végétales, INRA, BP 35327, 35653 Le Rheu Cedex, France. Regine.Delourme@rennes.inra.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18696043

Citation

Delourme, R, et al. "Molecular and Phenotypic Characterization of Near Isogenic Lines at QTL for Quantitative Resistance to Leptosphaeria Maculans in Oilseed Rape (Brassica Napus L.)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 117, no. 7, 2008, pp. 1055-67.
Delourme R, Piel N, Horvais R, et al. Molecular and phenotypic characterization of near isogenic lines at QTL for quantitative resistance to Leptosphaeria maculans in oilseed rape (Brassica napus L.). Theor Appl Genet. 2008;117(7):1055-67.
Delourme, R., Piel, N., Horvais, R., Pouilly, N., Domin, C., Vallée, P., Falentin, C., Manzanares-Dauleux, M. J., & Renard, M. (2008). Molecular and phenotypic characterization of near isogenic lines at QTL for quantitative resistance to Leptosphaeria maculans in oilseed rape (Brassica napus L.). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 117(7), 1055-67. https://doi.org/10.1007/s00122-008-0844-x
Delourme R, et al. Molecular and Phenotypic Characterization of Near Isogenic Lines at QTL for Quantitative Resistance to Leptosphaeria Maculans in Oilseed Rape (Brassica Napus L.). Theor Appl Genet. 2008;117(7):1055-67. PubMed PMID: 18696043.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular and phenotypic characterization of near isogenic lines at QTL for quantitative resistance to Leptosphaeria maculans in oilseed rape (Brassica napus L.). AU - Delourme,R, AU - Piel,N, AU - Horvais,R, AU - Pouilly,N, AU - Domin,C, AU - Vallée,P, AU - Falentin,C, AU - Manzanares-Dauleux,M J, AU - Renard,M, Y1 - 2008/08/12/ PY - 2008/03/13/received PY - 2008/06/28/accepted PY - 2008/06/20/revised PY - 2008/8/13/pubmed PY - 2009/2/7/medline PY - 2008/8/13/entrez SP - 1055 EP - 67 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor. Appl. Genet. VL - 117 IS - 7 N2 - The most common and effective way to control phoma stem canker (blackleg) caused by Leptosphaeria maculans in oilseed rape (Brassica napus) is by breeding resistant cultivars. Specific resistance genes have been identified in B. napus and related species but in some B. napus cultivars resistance is polygenic [mediated by quantitative trait loci (QTL)], postulated to be race non-specific and durable. The genetic basis of quantitative resistance in the French winter oilseed rape 'Darmor', which was derived from 'Jet Neuf', was previously examined in two genetic backgrounds. Stable QTL involved in blackleg resistance across year and genetic backgrounds were identified. In this study, near isogenic lines (NILs) were produced in the susceptible background 'Yudal' for four of these QTL using marker-assisted selection. Various strategies were used to develop new molecular markers, which were mapped in these QTL regions. These were used to characterize the length and homozygosity of the 'Darmor-bzh' introgressed segment in the NILs. Individuals from each NIL were evaluated in blackleg disease field trials and assessed for their level of stem canker in comparison to the recurrent line 'Yudal'. The effect of QTL LmA2 was clearly validated and to a lesser extent, QTL LmA9 also showed an effect on the disease level. This work provides valuable material that can be used to study the mode of action of genetic factors involved in L. maculans quantitative resistance. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/18696043/Molecular_and_phenotypic_characterization_of_near_isogenic_lines_at_QTL_for_quantitative_resistance_to_Leptosphaeria_maculans_in_oilseed_rape__Brassica_napus_L___ L2 - https://dx.doi.org/10.1007/s00122-008-0844-x DB - PRIME DP - Unbound Medicine ER -