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Replication and transmission of H9N2 influenza viruses in ferrets: evaluation of pandemic potential.
PLoS One. 2008 Aug 13; 3(8):e2923.Plos

Abstract

H9N2 avian influenza A viruses are endemic in poultry of many Eurasian countries and have caused repeated human infections in Asia since 1998. To evaluate the potential threat of H9N2 viruses to humans, we investigated the replication and transmission efficiency of H9N2 viruses in the ferret model. Five wild-type (WT) H9N2 viruses, isolated from different avian species from 1988 through 2003, were tested in vivo and found to replicate in ferrets. However these viruses achieved mild peak viral titers in nasal washes when compared to those observed with a human H3N2 virus. Two of these H9N2 viruses transmitted to direct contact ferrets, however no aerosol transmission was detected in the virus displaying the most efficient direct contact transmission. A leucine (Leu) residue at amino acid position 226 in the hemagglutinin (HA) receptor-binding site (RBS), responsible for human virus-like receptor specificity, was found to be important for the transmission of the H9N2 viruses in ferrets. In addition, an H9N2 avian-human reassortant virus, which contains the surface glycoprotein genes from an H9N2 virus and the six internal genes of a human H3N2 virus, showed enhanced replication and efficient transmission to direct contacts. Although no aerosol transmission was observed, the virus replicated in multiple respiratory tissues and induced clinical signs similar to those observed with the parental human H3N2 virus. Our results suggest that the establishment and prevalence of H9N2 viruses in poultry pose a significant threat for humans.

Authors+Show Affiliations

Department of Veterinary Medicine, University of Maryland, College Park, Maryland, United States of America.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

18698430

Citation

Wan, Hongquan, et al. "Replication and Transmission of H9N2 Influenza Viruses in Ferrets: Evaluation of Pandemic Potential." PloS One, vol. 3, no. 8, 2008, pp. e2923.
Wan H, Sorrell EM, Song H, et al. Replication and transmission of H9N2 influenza viruses in ferrets: evaluation of pandemic potential. PLoS ONE. 2008;3(8):e2923.
Wan, H., Sorrell, E. M., Song, H., Hossain, M. J., Ramirez-Nieto, G., Monne, I., Stevens, J., Cattoli, G., Capua, I., Chen, L. M., Donis, R. O., Busch, J., Paulson, J. C., Brockwell, C., Webby, R., Blanco, J., Al-Natour, M. Q., & Perez, D. R. (2008). Replication and transmission of H9N2 influenza viruses in ferrets: evaluation of pandemic potential. PloS One, 3(8), e2923. https://doi.org/10.1371/journal.pone.0002923
Wan H, et al. Replication and Transmission of H9N2 Influenza Viruses in Ferrets: Evaluation of Pandemic Potential. PLoS ONE. 2008 Aug 13;3(8):e2923. PubMed PMID: 18698430.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Replication and transmission of H9N2 influenza viruses in ferrets: evaluation of pandemic potential. AU - Wan,Hongquan, AU - Sorrell,Erin M, AU - Song,Haichen, AU - Hossain,Md Jaber, AU - Ramirez-Nieto,Gloria, AU - Monne,Isabella, AU - Stevens,James, AU - Cattoli,Giovanni, AU - Capua,Ilaria, AU - Chen,Li-Mei, AU - Donis,Ruben O, AU - Busch,Julia, AU - Paulson,James C, AU - Brockwell,Christy, AU - Webby,Richard, AU - Blanco,Jorge, AU - Al-Natour,Mohammad Q, AU - Perez,Daniel R, Y1 - 2008/08/13/ PY - 2008/05/15/received PY - 2008/07/14/accepted PY - 2008/8/14/pubmed PY - 2008/12/19/medline PY - 2008/8/14/entrez SP - e2923 EP - e2923 JF - PloS one JO - PLoS ONE VL - 3 IS - 8 N2 - H9N2 avian influenza A viruses are endemic in poultry of many Eurasian countries and have caused repeated human infections in Asia since 1998. To evaluate the potential threat of H9N2 viruses to humans, we investigated the replication and transmission efficiency of H9N2 viruses in the ferret model. Five wild-type (WT) H9N2 viruses, isolated from different avian species from 1988 through 2003, were tested in vivo and found to replicate in ferrets. However these viruses achieved mild peak viral titers in nasal washes when compared to those observed with a human H3N2 virus. Two of these H9N2 viruses transmitted to direct contact ferrets, however no aerosol transmission was detected in the virus displaying the most efficient direct contact transmission. A leucine (Leu) residue at amino acid position 226 in the hemagglutinin (HA) receptor-binding site (RBS), responsible for human virus-like receptor specificity, was found to be important for the transmission of the H9N2 viruses in ferrets. In addition, an H9N2 avian-human reassortant virus, which contains the surface glycoprotein genes from an H9N2 virus and the six internal genes of a human H3N2 virus, showed enhanced replication and efficient transmission to direct contacts. Although no aerosol transmission was observed, the virus replicated in multiple respiratory tissues and induced clinical signs similar to those observed with the parental human H3N2 virus. Our results suggest that the establishment and prevalence of H9N2 viruses in poultry pose a significant threat for humans. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/18698430/Replication_and_transmission_of_H9N2_influenza_viruses_in_ferrets:_evaluation_of_pandemic_potential_ L2 - http://dx.plos.org/10.1371/journal.pone.0002923 DB - PRIME DP - Unbound Medicine ER -