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Evaluation of on-line solid phase extraction-capillary electrophoresis-electrospray-mass spectrometry for the analysis of neuropeptides in human plasma.
Electrophoresis. 2008 Aug; 29(16):3366-76.E

Abstract

In this study, the suitability of SPE coupled on-line to CE-electrospray-MS (SPE-CE-ESI-MS) was evaluated for the analysis of neuropeptides in human plasma. First, CE-ESI-MS was investigated and a sample pretreatment based on precipitation with ACN was used for cleanup of plasma samples. The main quality parameters were determined and were consistent with those previously obtained for the analysis of standard mixtures, e.g., the LODs were of around 1 microg/mL. SPE-CE-ESI-MS employing microcartridges containing a C18 stationary phase was explored in order to decrease the LODs. A double-step sample cleanup pretreatment consisting of precipitation with ACN and ultrafiltration through 3000 Da MW cut-off membranes was needed to prevent the microcartridge saturation. Repeatability, reproducibility, linearity, and LODs of the SPE-CE-ESI-MS method and the durability of the microcartridges were studied. The LODs were markedly improved, ranging between 10 and 0.1 ng/mL depending on the peptide.

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Authors+Show Affiliations

Hernández E
Department of Analytical Chemistry, University of Barcelona, Barcelona, Spain.
Benavente F
No affiliation info available
Sanz-Nebot V
No affiliation info available
Barbosa J
No affiliation info available

MeSH

Blood ProteinsElectrophoresis, CapillaryHumansNeuropeptidesSpectrometry, Mass, Electrospray Ionization

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18702060

Citation

Hernández, Elena, et al. "Evaluation of On-line Solid Phase Extraction-capillary Electrophoresis-electrospray-mass Spectrometry for the Analysis of Neuropeptides in Human Plasma." Electrophoresis, vol. 29, no. 16, 2008, pp. 3366-76.
Hernández E, Benavente F, Sanz-Nebot V, et al. Evaluation of on-line solid phase extraction-capillary electrophoresis-electrospray-mass spectrometry for the analysis of neuropeptides in human plasma. Electrophoresis. 2008;29(16):3366-76.
Hernández, E., Benavente, F., Sanz-Nebot, V., & Barbosa, J. (2008). Evaluation of on-line solid phase extraction-capillary electrophoresis-electrospray-mass spectrometry for the analysis of neuropeptides in human plasma. Electrophoresis, 29(16), 3366-76. https://doi.org/10.1002/elps.200700872
Hernández E, et al. Evaluation of On-line Solid Phase Extraction-capillary Electrophoresis-electrospray-mass Spectrometry for the Analysis of Neuropeptides in Human Plasma. Electrophoresis. 2008;29(16):3366-76. PubMed PMID: 18702060.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of on-line solid phase extraction-capillary electrophoresis-electrospray-mass spectrometry for the analysis of neuropeptides in human plasma. AU - Hernández,Elena, AU - Benavente,Fernando, AU - Sanz-Nebot,Victoria, AU - Barbosa,José, PY - 2008/8/15/pubmed PY - 2008/12/23/medline PY - 2008/8/15/entrez SP - 3366 EP - 76 JF - Electrophoresis JO - Electrophoresis VL - 29 IS - 16 N2 - In this study, the suitability of SPE coupled on-line to CE-electrospray-MS (SPE-CE-ESI-MS) was evaluated for the analysis of neuropeptides in human plasma. First, CE-ESI-MS was investigated and a sample pretreatment based on precipitation with ACN was used for cleanup of plasma samples. The main quality parameters were determined and were consistent with those previously obtained for the analysis of standard mixtures, e.g., the LODs were of around 1 microg/mL. SPE-CE-ESI-MS employing microcartridges containing a C18 stationary phase was explored in order to decrease the LODs. A double-step sample cleanup pretreatment consisting of precipitation with ACN and ultrafiltration through 3000 Da MW cut-off membranes was needed to prevent the microcartridge saturation. Repeatability, reproducibility, linearity, and LODs of the SPE-CE-ESI-MS method and the durability of the microcartridges were studied. The LODs were markedly improved, ranging between 10 and 0.1 ng/mL depending on the peptide. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/18702060/Evaluation_of_on_line_solid_phase_extraction_capillary_electrophoresis_electrospray_mass_spectrometry_for_the_analysis_of_neuropeptides_in_human_plasma_ L2 - https://doi.org/10.1002/elps.200700872 DB - PRIME DP - Unbound Medicine ER -