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Silver nanorod arrays as a surface-enhanced Raman scattering substrate for foodborne pathogenic bacteria detection.
Appl Spectrosc. 2008 Aug; 62(8):922-31.AS

Abstract

Surface-enhanced Raman scattering (SERS) using novel silver nanorod array substrates has been used for the detection of pathogenic bacteria. The substrate consists of a base layer of 500 nm silver film on a glass slide and a layer of silver nanorod array with a length of approximately 1 microm produced by the oblique angle deposition method at a vapor incident angle of 86 degrees . Spectra from whole cell bacteria, Generic Escherichia coli, E. coli O157:H7, E. coli DH 5alpha, Staphylococcus aureus, S. epidermidis, and Salmonella typhimurium, and bacteria mixtures have been obtained. This SERS active substrate can detect spectral differences between Gram types, different species, their mixture, and strains. Principal component analysis (PCA) has been applied to classify the spectra. Viable and nonviable cells have also been examined, and significantly reduced SERS responses were observed for nonviable cells. SERS detection of bacteria at the single cell level, excited at low incident laser power (12 micro W) and short collection time (10 s), has also been demonstrated. These results indicate that the SERS-active silver nanorod array substrate is a potential analytical sensor for rapid identification of microorganisms with a minimum of sample preparation.

Authors+Show Affiliations

Department of Food Science and Technology, The University of Georgia, Athens, Georgia 30602, USA. vivichu@yahoo.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

18702867

Citation

Chu, Hsiaoyun, et al. "Silver Nanorod Arrays as a Surface-enhanced Raman Scattering Substrate for Foodborne Pathogenic Bacteria Detection." Applied Spectroscopy, vol. 62, no. 8, 2008, pp. 922-31.
Chu H, Huang Y, Zhao Y. Silver nanorod arrays as a surface-enhanced Raman scattering substrate for foodborne pathogenic bacteria detection. Appl Spectrosc. 2008;62(8):922-31.
Chu, H., Huang, Y., & Zhao, Y. (2008). Silver nanorod arrays as a surface-enhanced Raman scattering substrate for foodborne pathogenic bacteria detection. Applied Spectroscopy, 62(8), 922-31. https://doi.org/10.1366/000370208785284330
Chu H, Huang Y, Zhao Y. Silver Nanorod Arrays as a Surface-enhanced Raman Scattering Substrate for Foodborne Pathogenic Bacteria Detection. Appl Spectrosc. 2008;62(8):922-31. PubMed PMID: 18702867.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silver nanorod arrays as a surface-enhanced Raman scattering substrate for foodborne pathogenic bacteria detection. AU - Chu,Hsiaoyun, AU - Huang,Yaowen, AU - Zhao,Yiping, PY - 2008/8/16/pubmed PY - 2008/9/17/medline PY - 2008/8/16/entrez SP - 922 EP - 31 JF - Applied spectroscopy JO - Appl Spectrosc VL - 62 IS - 8 N2 - Surface-enhanced Raman scattering (SERS) using novel silver nanorod array substrates has been used for the detection of pathogenic bacteria. The substrate consists of a base layer of 500 nm silver film on a glass slide and a layer of silver nanorod array with a length of approximately 1 microm produced by the oblique angle deposition method at a vapor incident angle of 86 degrees . Spectra from whole cell bacteria, Generic Escherichia coli, E. coli O157:H7, E. coli DH 5alpha, Staphylococcus aureus, S. epidermidis, and Salmonella typhimurium, and bacteria mixtures have been obtained. This SERS active substrate can detect spectral differences between Gram types, different species, their mixture, and strains. Principal component analysis (PCA) has been applied to classify the spectra. Viable and nonviable cells have also been examined, and significantly reduced SERS responses were observed for nonviable cells. SERS detection of bacteria at the single cell level, excited at low incident laser power (12 micro W) and short collection time (10 s), has also been demonstrated. These results indicate that the SERS-active silver nanorod array substrate is a potential analytical sensor for rapid identification of microorganisms with a minimum of sample preparation. SN - 0003-7028 UR - https://www.unboundmedicine.com/medline/citation/18702867/Silver_nanorod_arrays_as_a_surface_enhanced_Raman_scattering_substrate_for_foodborne_pathogenic_bacteria_detection_ L2 - https://journals.sagepub.com/doi/10.1366/000370208785284330?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -