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Genotoxic pressure of vineyard pesticides in fish: field and mesocosm surveys.
Aquat Toxicol. 2008 Sep 17; 89(3):197-203.AT

Abstract

The present study deals with the genotoxicity assessment of vineyard pesticides in fish exposed in the field or in mesocosm conditions. Primary DNA damage was quantified as strand breaks using the single cell gel electrophoresis assay (Comet assay) applied to fish erythrocytes. In a first experiment, a significant genotoxic effect was observed following an upstream-downstream gradient in early life stages of brown trout (Salmo trutta fario) exposed in the Morcille River contaminated by a mixture of vineyard pesticides during three consecutive years. The pronounced response in terms of DNA damage reported in the present study could argue for a high sensitivity of fish early life stage and/or a high level of exposure to genotoxic compounds in the Morcille River. This stresses the interest in using trout larvae incubated in sediment bed to assess genotoxic compounds in the field. In a second experiment, adult European topminnow (Phoxinus phoxinus) were exposed in water running through artificial channels to a mixture of diuron and azoxystrobin, two of the main pesticides detected in the Morcille watershed. As compared with the unexposed channel, a 3-5-fold increase in the DNA damage was observed in fish exposed to chronic environmental pesticide concentrations (1-2 microg L(-1) for diuron and 0.5-1 microg L(-1) for axoxystrobin). A single 6h pulse of pesticide (14 microg L(-1) of diuron and 7 microg L(-1) of azoxystrobin) was applied to simulate transiently elevated chemical concentrations in the river following storm conditions. It did not increase genotoxicity. After a 1-month recovery period, DNA damage in exposed fish erythrocytes recovered to unexposed level, suggesting possible involvement of both repair mechanisms and cellular turnover in this transient response. This work highlights that vineyard treatment by pesticides and in particular diuron and azoxystrobin can represent a genotoxic threat to fish from contaminated watershed rivers.

Authors+Show Affiliations

Laboratoire des Sciences de l'Environnement, Université de Lyon, INRA-ENTPE, Vaulx en Velin, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18703238

Citation

Bony, S, et al. "Genotoxic Pressure of Vineyard Pesticides in Fish: Field and Mesocosm Surveys." Aquatic Toxicology (Amsterdam, Netherlands), vol. 89, no. 3, 2008, pp. 197-203.
Bony S, Gillet C, Bouchez A, et al. Genotoxic pressure of vineyard pesticides in fish: field and mesocosm surveys. Aquat Toxicol. 2008;89(3):197-203.
Bony, S., Gillet, C., Bouchez, A., Margoum, C., & Devaux, A. (2008). Genotoxic pressure of vineyard pesticides in fish: field and mesocosm surveys. Aquatic Toxicology (Amsterdam, Netherlands), 89(3), 197-203. https://doi.org/10.1016/j.aquatox.2008.06.017
Bony S, et al. Genotoxic Pressure of Vineyard Pesticides in Fish: Field and Mesocosm Surveys. Aquat Toxicol. 2008 Sep 17;89(3):197-203. PubMed PMID: 18703238.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotoxic pressure of vineyard pesticides in fish: field and mesocosm surveys. AU - Bony,S, AU - Gillet,C, AU - Bouchez,A, AU - Margoum,C, AU - Devaux,A, Y1 - 2008/07/09/ PY - 2008/05/05/received PY - 2008/06/26/revised PY - 2008/06/28/accepted PY - 2008/8/16/pubmed PY - 2008/11/5/medline PY - 2008/8/16/entrez SP - 197 EP - 203 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 89 IS - 3 N2 - The present study deals with the genotoxicity assessment of vineyard pesticides in fish exposed in the field or in mesocosm conditions. Primary DNA damage was quantified as strand breaks using the single cell gel electrophoresis assay (Comet assay) applied to fish erythrocytes. In a first experiment, a significant genotoxic effect was observed following an upstream-downstream gradient in early life stages of brown trout (Salmo trutta fario) exposed in the Morcille River contaminated by a mixture of vineyard pesticides during three consecutive years. The pronounced response in terms of DNA damage reported in the present study could argue for a high sensitivity of fish early life stage and/or a high level of exposure to genotoxic compounds in the Morcille River. This stresses the interest in using trout larvae incubated in sediment bed to assess genotoxic compounds in the field. In a second experiment, adult European topminnow (Phoxinus phoxinus) were exposed in water running through artificial channels to a mixture of diuron and azoxystrobin, two of the main pesticides detected in the Morcille watershed. As compared with the unexposed channel, a 3-5-fold increase in the DNA damage was observed in fish exposed to chronic environmental pesticide concentrations (1-2 microg L(-1) for diuron and 0.5-1 microg L(-1) for axoxystrobin). A single 6h pulse of pesticide (14 microg L(-1) of diuron and 7 microg L(-1) of azoxystrobin) was applied to simulate transiently elevated chemical concentrations in the river following storm conditions. It did not increase genotoxicity. After a 1-month recovery period, DNA damage in exposed fish erythrocytes recovered to unexposed level, suggesting possible involvement of both repair mechanisms and cellular turnover in this transient response. This work highlights that vineyard treatment by pesticides and in particular diuron and azoxystrobin can represent a genotoxic threat to fish from contaminated watershed rivers. SN - 0166-445X UR - https://www.unboundmedicine.com/medline/citation/18703238/Genotoxic_pressure_of_vineyard_pesticides_in_fish:_field_and_mesocosm_surveys_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(08)00209-9 DB - PRIME DP - Unbound Medicine ER -