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Prolonged antidiabetic effect of zinc-crystallized insulin loaded glycol chitosan nanoparticles in type 1 diabetic rats.
Arch Pharm Res. 2008 Jul; 31(7):918-23.AP

Abstract

New basal insulin formulation was designed and their structural characteristics were investigated in vitro and biological activities in type 1 diabetic rats. Zinc-crystallized insulin was physically loaded into hydrophobically modified glycol chitosan (HGC) nanoparticles by a dialysis method. The series of insulin-HGC formulations were prepared with different feed weight ratio of insulin to HGC from 0.5:1 to 4:1. The loading contents of insulin and size distribution of insulin-HGCs were characterized, and blood glucose responses were investigated in streptozotocin-induced diabetic rats after single subcutaneous injection of regular insulin and insulin-HGCs. The highest loading efficiency and content were obtained in insulin-HGC when a 1:1 feed weight ratio of insulin to HGC was employed. The hydrodynamic diameter of insulin-HGC nanoparticles were in the range of 200 to 500 nm with narrow size distribution. Insulin-HGC effectively sustained insulin release up to 40% within 12 hours followed by a slower controlled release. Insulin-HGC showed an extended blood glucose lowering effect up to 24 h and provided normal blood glucose levels after oral glucose (1.5 g/kg) load at 24 hours post-injection while regular insulin showed severe hypoglycemia. The prolonged time action profiles and low variability of insulin-HGC formulation resulted in improved blood glucose control in diabetic rats and fulfilled a pattern desirable of a basal insulin.

Authors+Show Affiliations

Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18704336

Citation

Jo, Hyung Gon, et al. "Prolonged Antidiabetic Effect of Zinc-crystallized Insulin Loaded Glycol Chitosan Nanoparticles in Type 1 Diabetic Rats." Archives of Pharmacal Research, vol. 31, no. 7, 2008, pp. 918-23.
Jo HG, Min KH, Nam TH, et al. Prolonged antidiabetic effect of zinc-crystallized insulin loaded glycol chitosan nanoparticles in type 1 diabetic rats. Arch Pharm Res. 2008;31(7):918-23.
Jo, H. G., Min, K. H., Nam, T. H., Na, S. J., Park, J. H., & Jeong, S. Y. (2008). Prolonged antidiabetic effect of zinc-crystallized insulin loaded glycol chitosan nanoparticles in type 1 diabetic rats. Archives of Pharmacal Research, 31(7), 918-23. https://doi.org/10.1007/s12272-001-1247-9
Jo HG, et al. Prolonged Antidiabetic Effect of Zinc-crystallized Insulin Loaded Glycol Chitosan Nanoparticles in Type 1 Diabetic Rats. Arch Pharm Res. 2008;31(7):918-23. PubMed PMID: 18704336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prolonged antidiabetic effect of zinc-crystallized insulin loaded glycol chitosan nanoparticles in type 1 diabetic rats. AU - Jo,Hyung Gon, AU - Min,Kyung Hyun, AU - Nam,Tae Hwan, AU - Na,Seong Ju, AU - Park,Jae Hyung, AU - Jeong,Seo Young, Y1 - 2008/08/14/ PY - 2008/01/03/received PY - 2008/06/10/accepted PY - 2008/06/03/revised PY - 2008/8/16/pubmed PY - 2008/9/17/medline PY - 2008/8/16/entrez SP - 918 EP - 23 JF - Archives of pharmacal research JO - Arch Pharm Res VL - 31 IS - 7 N2 - New basal insulin formulation was designed and their structural characteristics were investigated in vitro and biological activities in type 1 diabetic rats. Zinc-crystallized insulin was physically loaded into hydrophobically modified glycol chitosan (HGC) nanoparticles by a dialysis method. The series of insulin-HGC formulations were prepared with different feed weight ratio of insulin to HGC from 0.5:1 to 4:1. The loading contents of insulin and size distribution of insulin-HGCs were characterized, and blood glucose responses were investigated in streptozotocin-induced diabetic rats after single subcutaneous injection of regular insulin and insulin-HGCs. The highest loading efficiency and content were obtained in insulin-HGC when a 1:1 feed weight ratio of insulin to HGC was employed. The hydrodynamic diameter of insulin-HGC nanoparticles were in the range of 200 to 500 nm with narrow size distribution. Insulin-HGC effectively sustained insulin release up to 40% within 12 hours followed by a slower controlled release. Insulin-HGC showed an extended blood glucose lowering effect up to 24 h and provided normal blood glucose levels after oral glucose (1.5 g/kg) load at 24 hours post-injection while regular insulin showed severe hypoglycemia. The prolonged time action profiles and low variability of insulin-HGC formulation resulted in improved blood glucose control in diabetic rats and fulfilled a pattern desirable of a basal insulin. SN - 0253-6269 UR - https://www.unboundmedicine.com/medline/citation/18704336/Prolonged_antidiabetic_effect_of_zinc_crystallized_insulin_loaded_glycol_chitosan_nanoparticles_in_type_1_diabetic_rats_ L2 - https://dx.doi.org/10.1007/s12272-001-1247-9 DB - PRIME DP - Unbound Medicine ER -