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Evaluation of Candida albicans allergens reactive with specific IgE in asthma and atopic eczema patients.
Mycoses. 2009 Jul; 52(4):326-33.M

Abstract

Candida albicans (C. albicans) produces important allergenic components which can induce allergic reactions in sensitised patients. The purpose of this study was to extract the C. albicans antigens for evaluating the specific anti-Candida IgE in sera of atopic eczema (AE) and asthmatic patients (AS). 95 AE, 85 AS, and 70 non-atopic cases were selected with sequential trials. Candida albicans antigens were prepared and then skin prick test (SPT), ELISA and IgE-immunoblotting tests were performed for all patients. Positive SPT reactions were obtained on 52.6% of AE and 54.1% of AS patients and 4.3% of healthy controls (P < 0.05). Using ELISA, specific anti-C. albicans IgE antibody was detected in 32.6% and 41.2% of patients with AE and AS, respectively. No specific IgE antibody was detected in healthy controls (P < 0.05). In SDS-PAGE, protein bands with molecular weights between 13 and 135 kDa were detected, and some of them reacted with specific IgE in immunoblotting. In AE patients, the most important allergenic components were 25, 34, and 57 kDa protein bands, whereas in AS, 22, 25, and 34 kDa protein bands were observed as major allergens. Candida albicans produces different allergenic components that can induce allergic reactions and may be pathogenetically important in patients with AE and AS.

Authors+Show Affiliations

Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18705661

Citation

Khosravi, A R., et al. "Evaluation of Candida Albicans Allergens Reactive With Specific IgE in Asthma and Atopic Eczema Patients." Mycoses, vol. 52, no. 4, 2009, pp. 326-33.
Khosravi AR, Bandghorai AN, Moazzeni M, et al. Evaluation of Candida albicans allergens reactive with specific IgE in asthma and atopic eczema patients. Mycoses. 2009;52(4):326-33.
Khosravi, A. R., Bandghorai, A. N., Moazzeni, M., Shokri, H., Mansouri, P., & Mahmoudi, M. (2009). Evaluation of Candida albicans allergens reactive with specific IgE in asthma and atopic eczema patients. Mycoses, 52(4), 326-33. https://doi.org/10.1111/j.1439-0507.2008.01599.x
Khosravi AR, et al. Evaluation of Candida Albicans Allergens Reactive With Specific IgE in Asthma and Atopic Eczema Patients. Mycoses. 2009;52(4):326-33. PubMed PMID: 18705661.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of Candida albicans allergens reactive with specific IgE in asthma and atopic eczema patients. AU - Khosravi,A R, AU - Bandghorai,A Naseri, AU - Moazzeni,M, AU - Shokri,H, AU - Mansouri,P, AU - Mahmoudi,M, Y1 - 2008/08/13/ PY - 2008/8/19/entrez PY - 2008/8/19/pubmed PY - 2013/9/10/medline SP - 326 EP - 33 JF - Mycoses JO - Mycoses VL - 52 IS - 4 N2 - Candida albicans (C. albicans) produces important allergenic components which can induce allergic reactions in sensitised patients. The purpose of this study was to extract the C. albicans antigens for evaluating the specific anti-Candida IgE in sera of atopic eczema (AE) and asthmatic patients (AS). 95 AE, 85 AS, and 70 non-atopic cases were selected with sequential trials. Candida albicans antigens were prepared and then skin prick test (SPT), ELISA and IgE-immunoblotting tests were performed for all patients. Positive SPT reactions were obtained on 52.6% of AE and 54.1% of AS patients and 4.3% of healthy controls (P < 0.05). Using ELISA, specific anti-C. albicans IgE antibody was detected in 32.6% and 41.2% of patients with AE and AS, respectively. No specific IgE antibody was detected in healthy controls (P < 0.05). In SDS-PAGE, protein bands with molecular weights between 13 and 135 kDa were detected, and some of them reacted with specific IgE in immunoblotting. In AE patients, the most important allergenic components were 25, 34, and 57 kDa protein bands, whereas in AS, 22, 25, and 34 kDa protein bands were observed as major allergens. Candida albicans produces different allergenic components that can induce allergic reactions and may be pathogenetically important in patients with AE and AS. SN - 1439-0507 UR - https://www.unboundmedicine.com/medline/citation/18705661/Evaluation_of_Candida_albicans_allergens_reactive_with_specific_IgE_in_asthma_and_atopic_eczema_patients_ L2 - https://doi.org/10.1111/j.1439-0507.2008.01599.x DB - PRIME DP - Unbound Medicine ER -