Tags

Type your tag names separated by a space and hit enter

Development of a group selective molecularly imprinted polymers based solid phase extraction of malachite green from fish water and fish feed samples.
Anal Chim Acta. 2008 Aug 29; 624(2):317-25.AC

Abstract

A group selective molecularly imprinted solid phase extraction (MISPE) for malachite green (MG) from fish water and fish feed samples was developed. Using MG as template molecule, methacrylic acid as functional monomer, ethylene glycoldimethacrylate as linking agent and bulk polymerization as synthetic method, the molecularly imprinted polymers (MIPs) were synthesized and characterized with rebinding experiment. The Scatchard polt's analysis revealed that the template-polymer system showed the two-site binding behavior with dissociation constants of 0.3194 micromol L(-1) and 15.70 micromol L(-1), respectively. MG and two structurally related compounds, leucomalachite green (LMG) and crystal violet (CV) were employed for selectivity test. The MIPs exhibited the highest selective rebinding to MG, but also displayed 83.0% and 87.5% of cross-reactivity with LMG and CV, demonstrating that MIPs could be used as group recognition sorbents in solid phase extraction. The extraction conditions of MISPE column for MG were optimized. Tap water samples spiked with MG at concentration of 0.5-10 ng mL(-1) were extracted by MISPE column and analyzed by high performance liquid chromatography. The recoveries of MISPE column for MG extraction were found to be 76.8-93.7% with the relative standard deviations of 2.12-10.09%, indicating the feasibility of the prepared MIPs for MG extraction. No detectable MG was observed in one fish farming water sample and two fish feed samples; while the MG concentrations in two pet fishpond water samples were found at 1.50 ng mL(-1) and 0.67 ng mL(-1), respectively.

Authors+Show Affiliations

College of Chemistry, Sichuan University, Wangjiang Road 29, Chengdu 610064, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18706339

Citation

Li, Ya-hui, et al. "Development of a Group Selective Molecularly Imprinted Polymers Based Solid Phase Extraction of Malachite Green From Fish Water and Fish Feed Samples." Analytica Chimica Acta, vol. 624, no. 2, 2008, pp. 317-25.
Li YH, Yang T, Qi XL, et al. Development of a group selective molecularly imprinted polymers based solid phase extraction of malachite green from fish water and fish feed samples. Anal Chim Acta. 2008;624(2):317-25.
Li, Y. H., Yang, T., Qi, X. L., Qiao, Y. W., & Deng, A. P. (2008). Development of a group selective molecularly imprinted polymers based solid phase extraction of malachite green from fish water and fish feed samples. Analytica Chimica Acta, 624(2), 317-25. https://doi.org/10.1016/j.aca.2008.07.004
Li YH, et al. Development of a Group Selective Molecularly Imprinted Polymers Based Solid Phase Extraction of Malachite Green From Fish Water and Fish Feed Samples. Anal Chim Acta. 2008 Aug 29;624(2):317-25. PubMed PMID: 18706339.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a group selective molecularly imprinted polymers based solid phase extraction of malachite green from fish water and fish feed samples. AU - Li,Ya-hui, AU - Yang,Tao, AU - Qi,Xiao-ling, AU - Qiao,Yu-wei, AU - Deng,An-ping, Y1 - 2008/07/12/ PY - 2008/05/18/received PY - 2008/06/29/revised PY - 2008/07/01/accepted PY - 2008/8/19/pubmed PY - 2008/10/10/medline PY - 2008/8/19/entrez SP - 317 EP - 25 JF - Analytica chimica acta JO - Anal Chim Acta VL - 624 IS - 2 N2 - A group selective molecularly imprinted solid phase extraction (MISPE) for malachite green (MG) from fish water and fish feed samples was developed. Using MG as template molecule, methacrylic acid as functional monomer, ethylene glycoldimethacrylate as linking agent and bulk polymerization as synthetic method, the molecularly imprinted polymers (MIPs) were synthesized and characterized with rebinding experiment. The Scatchard polt's analysis revealed that the template-polymer system showed the two-site binding behavior with dissociation constants of 0.3194 micromol L(-1) and 15.70 micromol L(-1), respectively. MG and two structurally related compounds, leucomalachite green (LMG) and crystal violet (CV) were employed for selectivity test. The MIPs exhibited the highest selective rebinding to MG, but also displayed 83.0% and 87.5% of cross-reactivity with LMG and CV, demonstrating that MIPs could be used as group recognition sorbents in solid phase extraction. The extraction conditions of MISPE column for MG were optimized. Tap water samples spiked with MG at concentration of 0.5-10 ng mL(-1) were extracted by MISPE column and analyzed by high performance liquid chromatography. The recoveries of MISPE column for MG extraction were found to be 76.8-93.7% with the relative standard deviations of 2.12-10.09%, indicating the feasibility of the prepared MIPs for MG extraction. No detectable MG was observed in one fish farming water sample and two fish feed samples; while the MG concentrations in two pet fishpond water samples were found at 1.50 ng mL(-1) and 0.67 ng mL(-1), respectively. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/18706339/Development_of_a_group_selective_molecularly_imprinted_polymers_based_solid_phase_extraction_of_malachite_green_from_fish_water_and_fish_feed_samples_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(08)01285-3 DB - PRIME DP - Unbound Medicine ER -