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Increased intraepithelial T-cells in stable COPD.
Respir Med. 2008 Dec; 102(12):1812-8.RM

Abstract

BACKGROUND

The airway epithelium is the first line of defence in the response to inhaled particles and irritants. Chronic obstructive pulmonary disease (COPD) is an inflammatory disease characterised by an irreversible loss of lung function, with cigarette smoking as a major risk factor. Here, we address intraepithelial T-cells in COPD, as these cells are a distinct T-cell subtype thought to have important regulatory functions. We hypothesised that intraepithelial T-cells play a role in the response to lung irritants and that the T-cell populations would be altered and associated with signs of inflammation in COPD.

METHODS

Bronchoscopy with endobronchial mucosal biopsy sampling was performed in 22 patients (mean age; 57) with stable COPD (median FEV(1)% predicted: 51). Age- and smoking- matched smokers (S) with normal lung function (n=14) and age-matched non-smokers (NS) (n=15) served as controls. Airway inflammation was recorded visually using bronchitis index (BI). Biopsy specimens were processed into glycol methacrylate resin and inflammatory cells were stained immunohistochemically.

RESULTS

The number of intraepithelial CD4+ T-cells were significantly higher in COPD patients compared to smokers as well as trend towards significance in non-smokers (p=0.005 and p=0.036, respectively), whereas intraepithelial CD8+ T-cells number were increased in patients with COPD compared to non-smokers (p=0.017). Both patients with COPD and smokers had a higher BI than non-smokers (p<0.001 for both).

CONCLUSIONS

The present data suggest a role for intraepithelial CD4+ and CD8+ T-cells in stable COPD and indicate that T-cells are of importance in the long-term inflammatory response in COPD or, alternatively, play a regulatory role.

Authors+Show Affiliations

Department of Medicine, Division of Respiratory Medicine, Karolinska Institutet, Solna, Stockholm, Sweden. magnus.lofdahl@karolinska.seNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18706796

Citation

Löfdahl, Magnus J., et al. "Increased Intraepithelial T-cells in Stable COPD." Respiratory Medicine, vol. 102, no. 12, 2008, pp. 1812-8.
Löfdahl MJ, Roos-Engstrand E, Pourazar J, et al. Increased intraepithelial T-cells in stable COPD. Respir Med. 2008;102(12):1812-8.
Löfdahl, M. J., Roos-Engstrand, E., Pourazar, J., Bucht, A., Dahlen, B., Elmberger, G., Blomberg, A., & Sköld, C. M. (2008). Increased intraepithelial T-cells in stable COPD. Respiratory Medicine, 102(12), 1812-8. https://doi.org/10.1016/j.rmed.2008.06.013
Löfdahl MJ, et al. Increased Intraepithelial T-cells in Stable COPD. Respir Med. 2008;102(12):1812-8. PubMed PMID: 18706796.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased intraepithelial T-cells in stable COPD. AU - Löfdahl,Magnus J, AU - Roos-Engstrand,Ester, AU - Pourazar,Jamshid, AU - Bucht,Anders, AU - Dahlen,Barbro, AU - Elmberger,Göran, AU - Blomberg,Anders, AU - Sköld,C Magnus, Y1 - 2008/08/15/ PY - 2008/01/28/received PY - 2008/06/04/revised PY - 2008/06/19/accepted PY - 2008/8/19/pubmed PY - 2009/1/3/medline PY - 2008/8/19/entrez SP - 1812 EP - 8 JF - Respiratory medicine JO - Respir Med VL - 102 IS - 12 N2 - BACKGROUND: The airway epithelium is the first line of defence in the response to inhaled particles and irritants. Chronic obstructive pulmonary disease (COPD) is an inflammatory disease characterised by an irreversible loss of lung function, with cigarette smoking as a major risk factor. Here, we address intraepithelial T-cells in COPD, as these cells are a distinct T-cell subtype thought to have important regulatory functions. We hypothesised that intraepithelial T-cells play a role in the response to lung irritants and that the T-cell populations would be altered and associated with signs of inflammation in COPD. METHODS: Bronchoscopy with endobronchial mucosal biopsy sampling was performed in 22 patients (mean age; 57) with stable COPD (median FEV(1)% predicted: 51). Age- and smoking- matched smokers (S) with normal lung function (n=14) and age-matched non-smokers (NS) (n=15) served as controls. Airway inflammation was recorded visually using bronchitis index (BI). Biopsy specimens were processed into glycol methacrylate resin and inflammatory cells were stained immunohistochemically. RESULTS: The number of intraepithelial CD4+ T-cells were significantly higher in COPD patients compared to smokers as well as trend towards significance in non-smokers (p=0.005 and p=0.036, respectively), whereas intraepithelial CD8+ T-cells number were increased in patients with COPD compared to non-smokers (p=0.017). Both patients with COPD and smokers had a higher BI than non-smokers (p<0.001 for both). CONCLUSIONS: The present data suggest a role for intraepithelial CD4+ and CD8+ T-cells in stable COPD and indicate that T-cells are of importance in the long-term inflammatory response in COPD or, alternatively, play a regulatory role. SN - 1532-3064 UR - https://www.unboundmedicine.com/medline/citation/18706796/Increased_intraepithelial_T_cells_in_stable_COPD_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0954-6111(08)00215-1 DB - PRIME DP - Unbound Medicine ER -