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A novel hydrogen peroxide sensor based on the direct electron transfer of horseradish peroxidase immobilized on silica-hydroxyapatite hybrid film.
Biosens Bioelectron. 2009 Jan 01; 24(5):1141-5.BB

Abstract

The direct electron transfer of immobilized horseradish peroxidase (HRP) on silica-hydroxyapatite (HAp) hybrid film-modified glassy carbon electrode (GCE) and its application as H(2)O(2) biosensors were investigated. On silica/HRP-HAp/GCE, HRP displayed a fast electron transfer process accompanied with one proton participate in. This sensor exhibited an excellent electrocatalytic response to the reduction of H(2)O(2) without the aid of an electron mediator. The proposed biosensor showed good reproducibility and high sensitivity to H(2)O(2) with the detection limit of 0.35 microM. In the range of 1.0-100 microM, the catalytic reduction current of H(2)O(2) was proportional to H(2)O(2) concentration. The apparent Michaelis-Menten constant (k(m)(app)) of the biosensor was calculated to be 21.8 microM, exhibiting a high enzymatic activity and affinity for H(2)O(2).

Authors+Show Affiliations

College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18707863

Citation

Wang, Bin, et al. "A Novel Hydrogen Peroxide Sensor Based On the Direct Electron Transfer of Horseradish Peroxidase Immobilized On Silica-hydroxyapatite Hybrid Film." Biosensors & Bioelectronics, vol. 24, no. 5, 2009, pp. 1141-5.
Wang B, Zhang JJ, Pan ZY, et al. A novel hydrogen peroxide sensor based on the direct electron transfer of horseradish peroxidase immobilized on silica-hydroxyapatite hybrid film. Biosens Bioelectron. 2009;24(5):1141-5.
Wang, B., Zhang, J. J., Pan, Z. Y., Tao, X. Q., & Wang, H. S. (2009). A novel hydrogen peroxide sensor based on the direct electron transfer of horseradish peroxidase immobilized on silica-hydroxyapatite hybrid film. Biosensors & Bioelectronics, 24(5), 1141-5. https://doi.org/10.1016/j.bios.2008.06.053
Wang B, et al. A Novel Hydrogen Peroxide Sensor Based On the Direct Electron Transfer of Horseradish Peroxidase Immobilized On Silica-hydroxyapatite Hybrid Film. Biosens Bioelectron. 2009 Jan 1;24(5):1141-5. PubMed PMID: 18707863.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel hydrogen peroxide sensor based on the direct electron transfer of horseradish peroxidase immobilized on silica-hydroxyapatite hybrid film. AU - Wang,Bin, AU - Zhang,Jing-Jing, AU - Pan,Zhuang-Ying, AU - Tao,Xu-Quan, AU - Wang,Huai-Sheng, Y1 - 2008/07/10/ PY - 2008/04/12/received PY - 2008/06/22/revised PY - 2008/06/25/accepted PY - 2008/8/19/pubmed PY - 2009/3/10/medline PY - 2008/8/19/entrez SP - 1141 EP - 5 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 24 IS - 5 N2 - The direct electron transfer of immobilized horseradish peroxidase (HRP) on silica-hydroxyapatite (HAp) hybrid film-modified glassy carbon electrode (GCE) and its application as H(2)O(2) biosensors were investigated. On silica/HRP-HAp/GCE, HRP displayed a fast electron transfer process accompanied with one proton participate in. This sensor exhibited an excellent electrocatalytic response to the reduction of H(2)O(2) without the aid of an electron mediator. The proposed biosensor showed good reproducibility and high sensitivity to H(2)O(2) with the detection limit of 0.35 microM. In the range of 1.0-100 microM, the catalytic reduction current of H(2)O(2) was proportional to H(2)O(2) concentration. The apparent Michaelis-Menten constant (k(m)(app)) of the biosensor was calculated to be 21.8 microM, exhibiting a high enzymatic activity and affinity for H(2)O(2). SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/18707863/A_novel_hydrogen_peroxide_sensor_based_on_the_direct_electron_transfer_of_horseradish_peroxidase_immobilized_on_silica_hydroxyapatite_hybrid_film_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(08)00324-2 DB - PRIME DP - Unbound Medicine ER -