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High and low temperatures differently affect infection density and vertical transmission of male-killing Spiroplasma symbionts in Drosophila hosts.
Appl Environ Microbiol 2008; 74(19):6053-9AE

Abstract

We investigated the vertical transmission, reproductive phenotype, and infection density of a male-killing Spiroplasma symbiont in two Drosophila species under physiological high and low temperatures through successive host generations. In both the native host Drosophila nebulosa and the nonnative host Drosophila melanogaster, the symbiont infection and the male-killing phenotype were stably maintained at 25 degrees C, rapidly lost at 18 degrees C, and gradually lost at 28 degrees C. In the nonnative host, both the high and low temperatures significantly suppressed the infection density of the spiroplasma. In the native host, by contrast, the low temperature suppressed the infection density of the spiroplasma whereas the high temperature had little effect on the infection density. These results suggested that the low temperature suppresses both the infection density and the vertical transmission of the spiroplasma whereas the high temperature suppresses the vertical transmission preferentially. The spiroplasma density was consistently higher in the native host than in the nonnative host, suggesting that the host genotype may affect the infection density of the symbiont. The temperature- and genotype-dependent instability of the symbiont infection highlights a complex genotype-by-genotype-by-environment interaction and may be relevant to the low infection frequencies of the male-killing spiroplasmas in natural Drosophila populations.

Authors+Show Affiliations

Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8566, Japan. h-anbutsu@aist.go.jpNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18708518

Citation

Anbutsu, Hisashi, et al. "High and Low Temperatures Differently Affect Infection Density and Vertical Transmission of Male-killing Spiroplasma Symbionts in Drosophila Hosts." Applied and Environmental Microbiology, vol. 74, no. 19, 2008, pp. 6053-9.
Anbutsu H, Goto S, Fukatsu T. High and low temperatures differently affect infection density and vertical transmission of male-killing Spiroplasma symbionts in Drosophila hosts. Appl Environ Microbiol. 2008;74(19):6053-9.
Anbutsu, H., Goto, S., & Fukatsu, T. (2008). High and low temperatures differently affect infection density and vertical transmission of male-killing Spiroplasma symbionts in Drosophila hosts. Applied and Environmental Microbiology, 74(19), pp. 6053-9. doi:10.1128/AEM.01503-08.
Anbutsu H, Goto S, Fukatsu T. High and Low Temperatures Differently Affect Infection Density and Vertical Transmission of Male-killing Spiroplasma Symbionts in Drosophila Hosts. Appl Environ Microbiol. 2008;74(19):6053-9. PubMed PMID: 18708518.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High and low temperatures differently affect infection density and vertical transmission of male-killing Spiroplasma symbionts in Drosophila hosts. AU - Anbutsu,Hisashi, AU - Goto,Shunsuke, AU - Fukatsu,Takema, Y1 - 2008/08/15/ PY - 2008/8/19/pubmed PY - 2008/10/24/medline PY - 2008/8/19/entrez SP - 6053 EP - 9 JF - Applied and environmental microbiology JO - Appl. Environ. Microbiol. VL - 74 IS - 19 N2 - We investigated the vertical transmission, reproductive phenotype, and infection density of a male-killing Spiroplasma symbiont in two Drosophila species under physiological high and low temperatures through successive host generations. In both the native host Drosophila nebulosa and the nonnative host Drosophila melanogaster, the symbiont infection and the male-killing phenotype were stably maintained at 25 degrees C, rapidly lost at 18 degrees C, and gradually lost at 28 degrees C. In the nonnative host, both the high and low temperatures significantly suppressed the infection density of the spiroplasma. In the native host, by contrast, the low temperature suppressed the infection density of the spiroplasma whereas the high temperature had little effect on the infection density. These results suggested that the low temperature suppresses both the infection density and the vertical transmission of the spiroplasma whereas the high temperature suppresses the vertical transmission preferentially. The spiroplasma density was consistently higher in the native host than in the nonnative host, suggesting that the host genotype may affect the infection density of the symbiont. The temperature- and genotype-dependent instability of the symbiont infection highlights a complex genotype-by-genotype-by-environment interaction and may be relevant to the low infection frequencies of the male-killing spiroplasmas in natural Drosophila populations. SN - 1098-5336 UR - https://www.unboundmedicine.com/medline/citation/18708518/High_and_low_temperatures_differently_affect_infection_density_and_vertical_transmission_of_male_killing_Spiroplasma_symbionts_in_Drosophila_hosts_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=18708518 DB - PRIME DP - Unbound Medicine ER -