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Effects of increasing docosahexaenoic acid intake in human healthy volunteers on lymphocyte activation and monocyte apoptosis.
Br J Nutr. 2009 Mar; 101(6):852-8.BJ

Abstract

Dietary intake of long-chain n-3 PUFA has been reported to decrease several markers of lymphocyte activation and modulate monocyte susceptibility to apoptosis. However, most human studies examined the combined effect of DHA and EPA using relatively high daily amounts of n-3 PUFA. The present study investigated the effects of increasing doses of DHA added to the regular diet of human healthy volunteers on lymphocyte response to tetradecanoylphorbol acetate plus ionomycin activation, and on monocyte apoptosis induced by oxidized LDL. Eight subjects were supplemented with increasing daily doses of DHA (200, 400, 800, 1600 mg) in a TAG form containing DHA as the only PUFA, for 2 weeks each dose. DHA intake dose-dependently increased the proportion of DHA in mononuclear cell phospholipids, the augmentation being significant after 400 mg DHA/d. The tetradecanoylphorbol acetate plus ionomycin-stimulated IL-2 mRNA level started to increase after ingestion of 400 mg DHA/d, with a maximum after 800 mg intake, and was positively correlated (P < 0.003) with DHA enrichment in cell phospholipids. The treatment of monocytes by oxidized LDL before DHA supplementation drastically reduced mitochondrial membrane potential as compared with native LDL treatment. Oxidized LDL apoptotic effect was significantly attenuated after 400 mg DHA/d and the protective effect was maintained throughout the experiment, although to a lesser extent at higher doses. The present results show that supplementation of the human diet with low DHA dosages improves lymphocyte activability. It also increases monocyte resistance to oxidized LDL-induced apoptosis, which may be beneficial in the prevention of atherosclerosis.

Authors+Show Affiliations

INSERM U870, INSA-Lyon, 11 avenue Jean Capelle, Bât. Louis Pasteur, 69621 Villeurbanne Cedex, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18710607

Citation

Mebarek, Saïda, et al. "Effects of Increasing Docosahexaenoic Acid Intake in Human Healthy Volunteers On Lymphocyte Activation and Monocyte Apoptosis." The British Journal of Nutrition, vol. 101, no. 6, 2009, pp. 852-8.
Mebarek S, Ermak N, Benzaria A, et al. Effects of increasing docosahexaenoic acid intake in human healthy volunteers on lymphocyte activation and monocyte apoptosis. Br J Nutr. 2009;101(6):852-8.
Mebarek, S., Ermak, N., Benzaria, A., Vicca, S., Dubois, M., Némoz, G., Laville, M., Lacour, B., Véricel, E., Lagarde, M., & Prigent, A. F. (2009). Effects of increasing docosahexaenoic acid intake in human healthy volunteers on lymphocyte activation and monocyte apoptosis. The British Journal of Nutrition, 101(6), 852-8. https://doi.org/10.1017/S0007114508051520
Mebarek S, et al. Effects of Increasing Docosahexaenoic Acid Intake in Human Healthy Volunteers On Lymphocyte Activation and Monocyte Apoptosis. Br J Nutr. 2009;101(6):852-8. PubMed PMID: 18710607.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of increasing docosahexaenoic acid intake in human healthy volunteers on lymphocyte activation and monocyte apoptosis. AU - Mebarek,Saïda, AU - Ermak,Natalia, AU - Benzaria,Amal, AU - Vicca,Stéphanie, AU - Dubois,Madeleine, AU - Némoz,Georges, AU - Laville,Martine, AU - Lacour,Bernard, AU - Véricel,Evelyne, AU - Lagarde,Michel, AU - Prigent,Annie-France, Y1 - 2008/08/19/ PY - 2008/8/20/pubmed PY - 2009/9/26/medline PY - 2008/8/20/entrez SP - 852 EP - 8 JF - The British journal of nutrition JO - Br. J. Nutr. VL - 101 IS - 6 N2 - Dietary intake of long-chain n-3 PUFA has been reported to decrease several markers of lymphocyte activation and modulate monocyte susceptibility to apoptosis. However, most human studies examined the combined effect of DHA and EPA using relatively high daily amounts of n-3 PUFA. The present study investigated the effects of increasing doses of DHA added to the regular diet of human healthy volunteers on lymphocyte response to tetradecanoylphorbol acetate plus ionomycin activation, and on monocyte apoptosis induced by oxidized LDL. Eight subjects were supplemented with increasing daily doses of DHA (200, 400, 800, 1600 mg) in a TAG form containing DHA as the only PUFA, for 2 weeks each dose. DHA intake dose-dependently increased the proportion of DHA in mononuclear cell phospholipids, the augmentation being significant after 400 mg DHA/d. The tetradecanoylphorbol acetate plus ionomycin-stimulated IL-2 mRNA level started to increase after ingestion of 400 mg DHA/d, with a maximum after 800 mg intake, and was positively correlated (P < 0.003) with DHA enrichment in cell phospholipids. The treatment of monocytes by oxidized LDL before DHA supplementation drastically reduced mitochondrial membrane potential as compared with native LDL treatment. Oxidized LDL apoptotic effect was significantly attenuated after 400 mg DHA/d and the protective effect was maintained throughout the experiment, although to a lesser extent at higher doses. The present results show that supplementation of the human diet with low DHA dosages improves lymphocyte activability. It also increases monocyte resistance to oxidized LDL-induced apoptosis, which may be beneficial in the prevention of atherosclerosis. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/18710607/Effects_of_increasing_docosahexaenoic_acid_intake_in_human_healthy_volunteers_on_lymphocyte_activation_and_monocyte_apoptosis_ L2 - https://www.cambridge.org/core/product/identifier/S0007114508051520/type/journal_article DB - PRIME DP - Unbound Medicine ER -