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The invitro assessment of antibacterial effect of papaya seed extract against bacterial pathogens isolated from urine, wound and stool.
Ethiop Med J 2008; 46(1):71-7EM

Abstract

BACKGROUND

Carica papaya family Caricaceacae is one of the herbal remedies, which has recently become a subject of research focus. It is used in traditional medicine for variety of purposes in treating infectious and noninfectious diseases.

OBJECTIVE

The objective of this study was to assess the antibacterial effect of papaya seed extract against bacterial pathogens isolated from wound, urine and stool.

METHODS

This analytical experimental study was conducted in Jimma University, School of Medical Laboratory Technology, Microbiology laboratory between February to March 2005. The antibacterial activity of methanol extract of papaya seed was investigated against specific pathogenic bacteria isolated from wound, urine and stool by an agar dilution technique and the crude preparation was assessed by an agar diffusion technique. The growth or inhibition of control strains of Escherichia coli, Staphylococcus aureus, Salmonella typhi, and Pseudomonas aeruginosa as well as the clinical isolates of these bacteria were determined in growth media.

RESULTS

Results obtained in this study indicate that the minimum inhibitory concentration of papaya seed extract for 50% of the test bacteria was 18.38mg/ml and for S. typhi the MIC was at 11.8 mg/ml of extract. However, the growth inhibitory effect of papaya seed extract was not observed for P. aeruginosa up to 26.25 mg /ml of extract. Even though, the minimum bactericidal concentration is higher than the minimum inhibitory concentration of papaya seed extract (13.13 mg/ml, 11.8 mg/ml respectively) against S. typhi control and clinical isolates, the minimum bactericidal concentration for 50% of the tested bacteria was found to be similar with the minimum inhibitory concentration of the test bacteria,

CONCLUSION

Papaya seed could be used as an effective antibacterial agent for the tested organisms. Nevertheless, preclinical studies including invivo animal models and clinical trial on the effect of the seed are essential before advocating large-scale therapy.

Authors+Show Affiliations

Department of Microbiology and Parasitology, College of Medicine and Health Sciences, The University of Gondar, Gondar, Ethiopia.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18711992

Citation

Yismaw, Gizachew, et al. "The Invitro Assessment of Antibacterial Effect of Papaya Seed Extract Against Bacterial Pathogens Isolated From Urine, Wound and Stool." Ethiopian Medical Journal, vol. 46, no. 1, 2008, pp. 71-7.
Yismaw G, Tessema B, Mulu A, et al. The invitro assessment of antibacterial effect of papaya seed extract against bacterial pathogens isolated from urine, wound and stool. Ethiop Med J. 2008;46(1):71-7.
Yismaw, G., Tessema, B., Mulu, A., & Tiruneh, M. (2008). The invitro assessment of antibacterial effect of papaya seed extract against bacterial pathogens isolated from urine, wound and stool. Ethiopian Medical Journal, 46(1), pp. 71-7.
Yismaw G, et al. The Invitro Assessment of Antibacterial Effect of Papaya Seed Extract Against Bacterial Pathogens Isolated From Urine, Wound and Stool. Ethiop Med J. 2008;46(1):71-7. PubMed PMID: 18711992.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The invitro assessment of antibacterial effect of papaya seed extract against bacterial pathogens isolated from urine, wound and stool. AU - Yismaw,Gizachew, AU - Tessema,Belay, AU - Mulu,Andargachew, AU - Tiruneh,Moges, PY - 2008/8/21/pubmed PY - 2008/10/10/medline PY - 2008/8/21/entrez SP - 71 EP - 7 JF - Ethiopian medical journal JO - Ethiop. Med. J. VL - 46 IS - 1 N2 - BACKGROUND: Carica papaya family Caricaceacae is one of the herbal remedies, which has recently become a subject of research focus. It is used in traditional medicine for variety of purposes in treating infectious and noninfectious diseases. OBJECTIVE: The objective of this study was to assess the antibacterial effect of papaya seed extract against bacterial pathogens isolated from wound, urine and stool. METHODS: This analytical experimental study was conducted in Jimma University, School of Medical Laboratory Technology, Microbiology laboratory between February to March 2005. The antibacterial activity of methanol extract of papaya seed was investigated against specific pathogenic bacteria isolated from wound, urine and stool by an agar dilution technique and the crude preparation was assessed by an agar diffusion technique. The growth or inhibition of control strains of Escherichia coli, Staphylococcus aureus, Salmonella typhi, and Pseudomonas aeruginosa as well as the clinical isolates of these bacteria were determined in growth media. RESULTS: Results obtained in this study indicate that the minimum inhibitory concentration of papaya seed extract for 50% of the test bacteria was 18.38mg/ml and for S. typhi the MIC was at 11.8 mg/ml of extract. However, the growth inhibitory effect of papaya seed extract was not observed for P. aeruginosa up to 26.25 mg /ml of extract. Even though, the minimum bactericidal concentration is higher than the minimum inhibitory concentration of papaya seed extract (13.13 mg/ml, 11.8 mg/ml respectively) against S. typhi control and clinical isolates, the minimum bactericidal concentration for 50% of the tested bacteria was found to be similar with the minimum inhibitory concentration of the test bacteria, CONCLUSION: Papaya seed could be used as an effective antibacterial agent for the tested organisms. Nevertheless, preclinical studies including invivo animal models and clinical trial on the effect of the seed are essential before advocating large-scale therapy. SN - 0014-1755 UR - https://www.unboundmedicine.com/medline/citation/18711992/full_citation/The_invitro_assessment_of_antibacterial_effect_of_papaya_seed_extract_against_bacterial_pathogens_isolated_from_urine_wound_and_stool_ L2 - https://medlineplus.gov/bacterialinfections.html DB - PRIME DP - Unbound Medicine ER -