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Role of nitric oxide dependence on nitric oxide synthase-like activity in the water stress signaling of maize seedling.
J Integr Plant Biol. 2008 Apr; 50(4):435-42.JI

Abstract

Nitric oxide (NO) has been known as an important signal in plant antioxidative defense but its production and roles in water stress are less known. The present study investigated whether NO dependence on a NO synthase-like (NOS) activity is involved in the signaling of drought-induced protective responses in maize seedlings.NOS activity, rate of NO release and drought responses were analyzed when NO donor sodium nitroprusside (SNP), NO scavenger c-PTIO (2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) and NOS inhibitor L-NAME (NG-nitro-L-arginine methyl ester) were applied to both detached maize leaves and whole plants. Both NOS activity and the rate of NO release increased substantially under dehydration stress. The high NOS activity induced by c-PTIO as NO scavenger and NO accumulation inhibited by NOS inhibitor L-NAME in dehydration-treated maize seedlings indicated that most NO production under water deficit stress may be generated from NOS-like activity. After dehydration stress for 3 h, detached maize leaves pretreated with NO donor SNP maintained more water content than that of control leaves pretreated with water. This result was consistent with the decrease in the transpiration rate of SNP-treated leaves subjected to drought treatment for 3 h. Membrane permeability, a cell injury index, was lower in SNP-treated maize leaves under dehydration stress for 4 h when compared with the control leaves. Also, superoxide dismutase (SOD) activity of SNP combined drought treatment maize leaves was higher than that of drought treatment alone, indicating that exogenous NO treatment alleviated the water loss and oxidative damage of maize leaves under water deficit stress. When c-PTIO as a specific NO scavenger was applied, the effects of applied SNP were overridden. Treatment with L-NAME on leaves also led to higher membrane permeability, higher transpiration rate and lower SOD activities than those of control leaves, indicating that NOS-like activity was involved in the antioxidative defense under water stress. These results suggested that NO dependence on NOS-like activity serves as a signaling component in the induction of protective responses and is associated with drought tolerance in maize seedlings.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Hao GP
Department of Biological Science, Taishan Medical University, Taian 271000, China.
Xing Y
No affiliation info available
Zhang JH
No affiliation info available

MeSH

Cell Membrane PermeabilityDehydrationDisastersNitric OxideNitric Oxide SynthasePlant LeavesPlant TranspirationSeedlingsSignal TransductionSuperoxide DismutaseWaterZea mays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18713377

Citation

Hao, Gang-Ping, et al. "Role of Nitric Oxide Dependence On Nitric Oxide Synthase-like Activity in the Water Stress Signaling of Maize Seedling." Journal of Integrative Plant Biology, vol. 50, no. 4, 2008, pp. 435-42.
Hao GP, Xing Y, Zhang JH. Role of nitric oxide dependence on nitric oxide synthase-like activity in the water stress signaling of maize seedling. J Integr Plant Biol. 2008;50(4):435-42.
Hao, G. P., Xing, Y., & Zhang, J. H. (2008). Role of nitric oxide dependence on nitric oxide synthase-like activity in the water stress signaling of maize seedling. Journal of Integrative Plant Biology, 50(4), 435-42. https://doi.org/10.1111/j.1744-7909.2008.00637.x
Hao GP, Xing Y, Zhang JH. Role of Nitric Oxide Dependence On Nitric Oxide Synthase-like Activity in the Water Stress Signaling of Maize Seedling. J Integr Plant Biol. 2008;50(4):435-42. PubMed PMID: 18713377.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of nitric oxide dependence on nitric oxide synthase-like activity in the water stress signaling of maize seedling. AU - Hao,Gang-Ping, AU - Xing,Yu, AU - Zhang,Jian-Hua, PY - 2008/8/21/pubmed PY - 2008/11/13/medline PY - 2008/8/21/entrez SP - 435 EP - 42 JF - Journal of integrative plant biology JO - J Integr Plant Biol VL - 50 IS - 4 N2 - Nitric oxide (NO) has been known as an important signal in plant antioxidative defense but its production and roles in water stress are less known. The present study investigated whether NO dependence on a NO synthase-like (NOS) activity is involved in the signaling of drought-induced protective responses in maize seedlings.NOS activity, rate of NO release and drought responses were analyzed when NO donor sodium nitroprusside (SNP), NO scavenger c-PTIO (2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) and NOS inhibitor L-NAME (NG-nitro-L-arginine methyl ester) were applied to both detached maize leaves and whole plants. Both NOS activity and the rate of NO release increased substantially under dehydration stress. The high NOS activity induced by c-PTIO as NO scavenger and NO accumulation inhibited by NOS inhibitor L-NAME in dehydration-treated maize seedlings indicated that most NO production under water deficit stress may be generated from NOS-like activity. After dehydration stress for 3 h, detached maize leaves pretreated with NO donor SNP maintained more water content than that of control leaves pretreated with water. This result was consistent with the decrease in the transpiration rate of SNP-treated leaves subjected to drought treatment for 3 h. Membrane permeability, a cell injury index, was lower in SNP-treated maize leaves under dehydration stress for 4 h when compared with the control leaves. Also, superoxide dismutase (SOD) activity of SNP combined drought treatment maize leaves was higher than that of drought treatment alone, indicating that exogenous NO treatment alleviated the water loss and oxidative damage of maize leaves under water deficit stress. When c-PTIO as a specific NO scavenger was applied, the effects of applied SNP were overridden. Treatment with L-NAME on leaves also led to higher membrane permeability, higher transpiration rate and lower SOD activities than those of control leaves, indicating that NOS-like activity was involved in the antioxidative defense under water stress. These results suggested that NO dependence on NOS-like activity serves as a signaling component in the induction of protective responses and is associated with drought tolerance in maize seedlings. SN - 1744-7909 UR - https://www.unboundmedicine.com/medline/citation/18713377/Role_of_nitric_oxide_dependence_on_nitric_oxide_synthase_like_activity_in_the_water_stress_signaling_of_maize_seedling_ L2 - https://doi.org/10.1111/j.1744-7909.2008.00637.x DB - PRIME DP - Unbound Medicine ER -