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Cell-wall invertases from rice are differentially expressed in Caryopsis during the grain filling stage.
J Integr Plant Biol. 2008 Apr; 50(4):466-74.JI

Abstract

Cell-wall invertase plays an important role in sucrose partitioning between source and sink organs in higher plants. To investigate the role of cell-wall invertases for seed development in rice (Oryza sativa L.), cDNAs of three putative cell-wall invertase genes OsCIN1, OsCIN2 and OsCIN3 were isolated. Semi-quantitative reverse transcription-polymerase chain reaction analysis revealed different expression patterns of the three genes in various rice tissues/organs. In developing caryopses, they exhibited similar temporal expression patterns, expressed highly at the early and middle grain filling stages and gradually declined to low levels afterward. However, the spatial expression patterns of them were very different, with OsCIN1 primarily expressed in the caryopsis coat, OsCIN2 in embryo and endosperm, and OsCIN3 in embryo. Further RNA in situ hybridization analysis revealed that a strong signal of OsCIN2 mRNA was detected in the vascular parenchyma surrounding the xylem of the chalazal vein and the aleurone layer, whereas OsCIN3 transcript was strongly detected in the vascular parenchyma surrounding the phloem of the chalazal vein, cross-cells, the aleurone layer and the nucellar tissue. These data indicate that the three cell-wall invertase genes play complementary/synergetic roles in assimilate unloading during the grain filling stage. In addition, the cell type-specific expression patterns of OsCIN3 in source leaf blades and anthers were also investigated, and its corresponding physiological roles were discussed.

Authors+Show Affiliations

State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing 100101, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18713381

Citation

Wang, Yong-Qin, et al. "Cell-wall Invertases From Rice Are Differentially Expressed in Caryopsis During the Grain Filling Stage." Journal of Integrative Plant Biology, vol. 50, no. 4, 2008, pp. 466-74.
Wang YQ, Wei XL, Xu HL, et al. Cell-wall invertases from rice are differentially expressed in Caryopsis during the grain filling stage. J Integr Plant Biol. 2008;50(4):466-74.
Wang, Y. Q., Wei, X. L., Xu, H. L., Chai, C. L., Meng, K., Zhai, H. L., Sun, A. J., Peng, Y. G., Wu, B., Xiao, G. F., & Zhu, Z. (2008). Cell-wall invertases from rice are differentially expressed in Caryopsis during the grain filling stage. Journal of Integrative Plant Biology, 50(4), 466-74. https://doi.org/10.1111/j.1744-7909.2008.00641.x
Wang YQ, et al. Cell-wall Invertases From Rice Are Differentially Expressed in Caryopsis During the Grain Filling Stage. J Integr Plant Biol. 2008;50(4):466-74. PubMed PMID: 18713381.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cell-wall invertases from rice are differentially expressed in Caryopsis during the grain filling stage. AU - Wang,Yong-Qin, AU - Wei,Xiao-Li, AU - Xu,Hong-Lin, AU - Chai,Cheng-Lin, AU - Meng,Kun, AU - Zhai,Hong-Li, AU - Sun,Ai-Jun, AU - Peng,Yong-Gang, AU - Wu,Bin, AU - Xiao,Gui-Fang, AU - Zhu,Zhen, PY - 2008/8/21/pubmed PY - 2008/11/13/medline PY - 2008/8/21/entrez SP - 466 EP - 74 JF - Journal of integrative plant biology JO - J Integr Plant Biol VL - 50 IS - 4 N2 - Cell-wall invertase plays an important role in sucrose partitioning between source and sink organs in higher plants. To investigate the role of cell-wall invertases for seed development in rice (Oryza sativa L.), cDNAs of three putative cell-wall invertase genes OsCIN1, OsCIN2 and OsCIN3 were isolated. Semi-quantitative reverse transcription-polymerase chain reaction analysis revealed different expression patterns of the three genes in various rice tissues/organs. In developing caryopses, they exhibited similar temporal expression patterns, expressed highly at the early and middle grain filling stages and gradually declined to low levels afterward. However, the spatial expression patterns of them were very different, with OsCIN1 primarily expressed in the caryopsis coat, OsCIN2 in embryo and endosperm, and OsCIN3 in embryo. Further RNA in situ hybridization analysis revealed that a strong signal of OsCIN2 mRNA was detected in the vascular parenchyma surrounding the xylem of the chalazal vein and the aleurone layer, whereas OsCIN3 transcript was strongly detected in the vascular parenchyma surrounding the phloem of the chalazal vein, cross-cells, the aleurone layer and the nucellar tissue. These data indicate that the three cell-wall invertase genes play complementary/synergetic roles in assimilate unloading during the grain filling stage. In addition, the cell type-specific expression patterns of OsCIN3 in source leaf blades and anthers were also investigated, and its corresponding physiological roles were discussed. SN - 1744-7909 UR - https://www.unboundmedicine.com/medline/citation/18713381/Cell_wall_invertases_from_rice_are_differentially_expressed_in_Caryopsis_during_the_grain_filling_stage_ L2 - https://doi.org/10.1111/j.1744-7909.2008.00641.x DB - PRIME DP - Unbound Medicine ER -