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Regulation of expression of microvillus membrane proteins by estrogen in baboon fetal ovarian oocytes.
Biol Reprod. 2008 Dec; 79(6):1160-8.BR

Abstract

We previously demonstrated that the number and height of oocyte microvilli were reduced in baboon fetuses deprived of estrogen in utero and restored to normal in animals supplemented with estradiol. Phosphorylated ezrin and Na+/H+ exchange regulatory factor 1 (NHERF, now termed SLC9A3R1) link f-actin bundles to the membrane, whereas alpha-actinin cross-links f-actin to form microvilli. Therefore, we determined whether these proteins were expressed in oocytes of the fetal baboon ovary and whether expression and/or localization were altered between mid and late gestation in association with an increase in estrogen and in late gestation in animals in which estrogen was suppressed (>95%) or restored by treatment with an aromatase inhibitor with or without estradiol. Expression of alpha-actinin was low at mid gestation, increased on the surface of oocytes of primordial follicles in late gestation, and was negligible in the ovaries of estrogen-suppressed fetuses and normal in animals treated with estrogen. Ezrin (total and phosphorylated) and SLC9A3R1 expression was localized to the surface of oocytes at mid and late gestation in estrogen-replete baboons and to the cytoplasm in late gestation after estrogen suppression. These results are the first to show that the fetal baboon oocyte expressed ezrin, SLC9A3R1, and alpha-actinin, and that these proteins were localized to the oocyte surface consistent with their role in microvilli development in epithelial cells. The current study also showed that the developmental increase in oocyte expression of alpha-actinin is regulated by estrogen and correlated with the estrogen-dependent increase in oocyte microvilli demonstrated previously. Therefore, we propose that development of oocyte microvilli requires expression of alpha-actinin and that expression of alpha-actinin and localization of ezrin-phosphate and SLC9A3R1 to the oocyte membrane are regulated by estrogen.

Authors+Show Affiliations

Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, Virginia 23501, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

18716290

Citation

Zachos, Nicholas C., et al. "Regulation of Expression of Microvillus Membrane Proteins By Estrogen in Baboon Fetal Ovarian Oocytes." Biology of Reproduction, vol. 79, no. 6, 2008, pp. 1160-8.
Zachos NC, Burch MG, Billiar RB, et al. Regulation of expression of microvillus membrane proteins by estrogen in baboon fetal ovarian oocytes. Biol Reprod. 2008;79(6):1160-8.
Zachos, N. C., Burch, M. G., Billiar, R. B., Li, C., Albrecht, E. D., & Pepe, G. J. (2008). Regulation of expression of microvillus membrane proteins by estrogen in baboon fetal ovarian oocytes. Biology of Reproduction, 79(6), 1160-8. https://doi.org/10.1095/biolreprod.108.067900
Zachos NC, et al. Regulation of Expression of Microvillus Membrane Proteins By Estrogen in Baboon Fetal Ovarian Oocytes. Biol Reprod. 2008;79(6):1160-8. PubMed PMID: 18716290.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of expression of microvillus membrane proteins by estrogen in baboon fetal ovarian oocytes. AU - Zachos,Nicholas C, AU - Burch,Marcia G, AU - Billiar,Reinhart B, AU - Li,Chunhua, AU - Albrecht,Eugene D, AU - Pepe,Gerald J, Y1 - 2008/08/20/ PY - 2008/8/22/pubmed PY - 2009/1/7/medline PY - 2008/8/22/entrez SP - 1160 EP - 8 JF - Biology of reproduction JO - Biol Reprod VL - 79 IS - 6 N2 - We previously demonstrated that the number and height of oocyte microvilli were reduced in baboon fetuses deprived of estrogen in utero and restored to normal in animals supplemented with estradiol. Phosphorylated ezrin and Na+/H+ exchange regulatory factor 1 (NHERF, now termed SLC9A3R1) link f-actin bundles to the membrane, whereas alpha-actinin cross-links f-actin to form microvilli. Therefore, we determined whether these proteins were expressed in oocytes of the fetal baboon ovary and whether expression and/or localization were altered between mid and late gestation in association with an increase in estrogen and in late gestation in animals in which estrogen was suppressed (>95%) or restored by treatment with an aromatase inhibitor with or without estradiol. Expression of alpha-actinin was low at mid gestation, increased on the surface of oocytes of primordial follicles in late gestation, and was negligible in the ovaries of estrogen-suppressed fetuses and normal in animals treated with estrogen. Ezrin (total and phosphorylated) and SLC9A3R1 expression was localized to the surface of oocytes at mid and late gestation in estrogen-replete baboons and to the cytoplasm in late gestation after estrogen suppression. These results are the first to show that the fetal baboon oocyte expressed ezrin, SLC9A3R1, and alpha-actinin, and that these proteins were localized to the oocyte surface consistent with their role in microvilli development in epithelial cells. The current study also showed that the developmental increase in oocyte expression of alpha-actinin is regulated by estrogen and correlated with the estrogen-dependent increase in oocyte microvilli demonstrated previously. Therefore, we propose that development of oocyte microvilli requires expression of alpha-actinin and that expression of alpha-actinin and localization of ezrin-phosphate and SLC9A3R1 to the oocyte membrane are regulated by estrogen. SN - 0006-3363 UR - https://www.unboundmedicine.com/medline/citation/18716290/Regulation_of_expression_of_microvillus_membrane_proteins_by_estrogen_in_baboon_fetal_ovarian_oocytes_ L2 - https://academic.oup.com/biolreprod/article-lookup/doi/10.1095/biolreprod.108.067900 DB - PRIME DP - Unbound Medicine ER -