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Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress.
J Vet Sci. 2008 Sep; 9(3):247-56.JV

Abstract

Oxidative stresses induced by reactive oxygen species (ROS) have been shown to be involved in several physiological and pathophysiological processes, such as cell proliferation and differentiation. Steroid hormones can protect cells against apoptosis or induce cell proliferation by several mechanisms. Among androgenic hormones, dihydrotestosterone (DHT) is generated by a 5alpha- reduction of testosterone. Unlike testosterone, DHT cannot be aromatized to estradiol, therefore DHT is considered a pure androgenic steroid. This study was conducted to examine the effect of DHT (10(-7) M) on H2O2 (10(-3) M) -induced injuries in mouse embryonic stem (ES) cells. H2O2 induced ROS generation and increased lipid peroxide formation and DNA fragmentation. These effects of H2O2 were inhibited by pretreatment with DHT. H2O2 also increased the phosphorylation of p38 MAPK, SAPK/JNK and nuclear factor kappa B (NF-kappaB), but DHT blocked these effects. Moreover, H2O2 decreased DNA synthesis and the levels of cell cycle regulatory proteins [cyclin D1, cyclin E, cyclin-dependent kinase (CDK) 2, and CDK 4]. These effects of H2O2 were inhibited by pretreatment with DHT. In conclusion, DHT may partially prevent H2O2-induced cell injury through inhibition of ROS and ROS-induced activation of p38 MAPK, SAPK/JNK and NF-kappaB in mouse ES cells.

Authors+Show Affiliations

Department of Urology, Chonnam National University Medical School, Gwangju 501-746, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18716444

Citation

Lee, Mi Na, et al. "Effect of Dihydrotestosterone On Mouse Embryonic Stem Cells Exposed to H2O2-induced Oxidative Stress." Journal of Veterinary Science, vol. 9, no. 3, 2008, pp. 247-56.
Lee MN, Lee SH, Lee MY, et al. Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress. J Vet Sci. 2008;9(3):247-56.
Lee, M. N., Lee, S. H., Lee, M. Y., Kim, Y. H., Park, J. H., Ryu, J. M., Yun, S. P., Lee, Y. J., Kim, M. O., Park, K., & Han, H. J. (2008). Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress. Journal of Veterinary Science, 9(3), 247-56.
Lee MN, et al. Effect of Dihydrotestosterone On Mouse Embryonic Stem Cells Exposed to H2O2-induced Oxidative Stress. J Vet Sci. 2008;9(3):247-56. PubMed PMID: 18716444.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress. AU - Lee,Mi Na, AU - Lee,Sang Hun, AU - Lee,Min Young, AU - Kim,Yun Hee, AU - Park,Jae Hong, AU - Ryu,Jung Min, AU - Yun,Seung Pil, AU - Lee,Yu Jin, AU - Kim,Mi Ok, AU - Park,Kwangsung, AU - Han,Ho Jae, PY - 2008/8/22/pubmed PY - 2008/11/14/medline PY - 2008/8/22/entrez SP - 247 EP - 56 JF - Journal of veterinary science JO - J Vet Sci VL - 9 IS - 3 N2 - Oxidative stresses induced by reactive oxygen species (ROS) have been shown to be involved in several physiological and pathophysiological processes, such as cell proliferation and differentiation. Steroid hormones can protect cells against apoptosis or induce cell proliferation by several mechanisms. Among androgenic hormones, dihydrotestosterone (DHT) is generated by a 5alpha- reduction of testosterone. Unlike testosterone, DHT cannot be aromatized to estradiol, therefore DHT is considered a pure androgenic steroid. This study was conducted to examine the effect of DHT (10(-7) M) on H2O2 (10(-3) M) -induced injuries in mouse embryonic stem (ES) cells. H2O2 induced ROS generation and increased lipid peroxide formation and DNA fragmentation. These effects of H2O2 were inhibited by pretreatment with DHT. H2O2 also increased the phosphorylation of p38 MAPK, SAPK/JNK and nuclear factor kappa B (NF-kappaB), but DHT blocked these effects. Moreover, H2O2 decreased DNA synthesis and the levels of cell cycle regulatory proteins [cyclin D1, cyclin E, cyclin-dependent kinase (CDK) 2, and CDK 4]. These effects of H2O2 were inhibited by pretreatment with DHT. In conclusion, DHT may partially prevent H2O2-induced cell injury through inhibition of ROS and ROS-induced activation of p38 MAPK, SAPK/JNK and NF-kappaB in mouse ES cells. SN - 1229-845X UR - https://www.unboundmedicine.com/medline/citation/18716444/Effect_of_dihydrotestosterone_on_mouse_embryonic_stem_cells_exposed_to_H2O2_induced_oxidative_stress_ L2 - https://www.vetsci.org/DOIx.php?id=10.4142/jvs.2008.9.3.247 DB - PRIME DP - Unbound Medicine ER -