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Study of the anti-proliferative effects and synergy of phthalides from Angelica sinensis on colon cancer cells.
J Ethnopharmacol. 2008 Oct 30; 120(1):36-43.JE

Abstract

Angelica sinensis is a Chinese medicinal herb for treating gynecological and gastrointestinal disorders, and also in conjunction with cancer chemotherapy.

AIM OF THE STUDY

In the present study, the cytotoxic and anti-proliferative effects of three main Angelica sinensis phthalides, namely n-butylidenephthalide (BLP), senkyunolide A (SKA) and z-ligustilide (LGT), and their synergy on colon cancer HT-29 cells were investigated. Moreover, the results obtained in both human colon cancer HT-29 and normal colon CCD-18Co cells were compared for the investigation of selectivity.

MATERIALS AND METHODS

MTT and [3H] thymidine incorporation assays were used for the evaluation of cytotoxic and anti-proliferative effects, respectively. Interactions among phthalides were determined by median-effect analysis.

RESULTS

All three phthalides dose-dependently decreased cell viability more potently in HT-29 than in CCD-18Co cells. The IC50 values for inhibition of cell proliferation for SKA, LGT and BLP were 54.17+/-5.10, 60.63+/-6.79 and 236.90+/-18.22microM, respectively, in HT-29 cells. Angelica sinensis extract demonstrated significant synergy in inhibiting cell proliferation.

CONCLUSIONS

The three phthalides might have anti-cancer potential, yet the phthalides, in combination with other ingredients in Angelica sinensis extract, display significant synergy leading to a stronger anti-tumor effect.

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Authors+Show Affiliations

Kan WL
Department of Pharmacology, Faculty of Medicine, Chinese University of Hong Kong, Basic Medical Sciences Building, Shatin, New Territories, Hong Kong S.A.R., China.
Cho CH
No affiliation info available
Rudd JA
No affiliation info available
Lin G
No affiliation info available

MeSH

AdenocarcinomaAngelicaAntineoplastic Agents, PhytogenicBenzofuransCell LineCell ProliferationCell SurvivalColorectal NeoplasmsDose-Response Relationship, DrugDrug SynergismDrugs, Chinese HerbalHT29 CellsHumansInhibitory Concentration 50Plant Extracts

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18718517

Citation

Kan, Winnie Lai Ting, et al. "Study of the Anti-proliferative Effects and Synergy of Phthalides From Angelica Sinensis On Colon Cancer Cells." Journal of Ethnopharmacology, vol. 120, no. 1, 2008, pp. 36-43.
Kan WL, Cho CH, Rudd JA, et al. Study of the anti-proliferative effects and synergy of phthalides from Angelica sinensis on colon cancer cells. J Ethnopharmacol. 2008;120(1):36-43.
Kan, W. L., Cho, C. H., Rudd, J. A., & Lin, G. (2008). Study of the anti-proliferative effects and synergy of phthalides from Angelica sinensis on colon cancer cells. Journal of Ethnopharmacology, 120(1), 36-43. https://doi.org/10.1016/j.jep.2008.07.027
Kan WL, et al. Study of the Anti-proliferative Effects and Synergy of Phthalides From Angelica Sinensis On Colon Cancer Cells. J Ethnopharmacol. 2008 Oct 30;120(1):36-43. PubMed PMID: 18718517.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Study of the anti-proliferative effects and synergy of phthalides from Angelica sinensis on colon cancer cells. AU - Kan,Winnie Lai Ting, AU - Cho,Chi Hin, AU - Rudd,John A, AU - Lin,Ge, Y1 - 2008/07/30/ PY - 2007/12/05/received PY - 2008/07/18/revised PY - 2008/07/22/accepted PY - 2008/8/23/pubmed PY - 2009/1/28/medline PY - 2008/8/23/entrez SP - 36 EP - 43 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 120 IS - 1 N2 - UNLABELLED: Angelica sinensis is a Chinese medicinal herb for treating gynecological and gastrointestinal disorders, and also in conjunction with cancer chemotherapy. AIM OF THE STUDY: In the present study, the cytotoxic and anti-proliferative effects of three main Angelica sinensis phthalides, namely n-butylidenephthalide (BLP), senkyunolide A (SKA) and z-ligustilide (LGT), and their synergy on colon cancer HT-29 cells were investigated. Moreover, the results obtained in both human colon cancer HT-29 and normal colon CCD-18Co cells were compared for the investigation of selectivity. MATERIALS AND METHODS: MTT and [3H] thymidine incorporation assays were used for the evaluation of cytotoxic and anti-proliferative effects, respectively. Interactions among phthalides were determined by median-effect analysis. RESULTS: All three phthalides dose-dependently decreased cell viability more potently in HT-29 than in CCD-18Co cells. The IC50 values for inhibition of cell proliferation for SKA, LGT and BLP were 54.17+/-5.10, 60.63+/-6.79 and 236.90+/-18.22microM, respectively, in HT-29 cells. Angelica sinensis extract demonstrated significant synergy in inhibiting cell proliferation. CONCLUSIONS: The three phthalides might have anti-cancer potential, yet the phthalides, in combination with other ingredients in Angelica sinensis extract, display significant synergy leading to a stronger anti-tumor effect. SN - 0378-8741 UR - https://www.unboundmedicine.com/medline/citation/18718517/Study_of_the_anti_proliferative_effects_and_synergy_of_phthalides_from_Angelica_sinensis_on_colon_cancer_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(08)00408-X DB - PRIME DP - Unbound Medicine ER -