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Direct assessments of the antioxidant effects of propofol medium chain triglyceride/long chain triglyceride on the brain of stroke-prone spontaneously hypertensive rats using electron spin resonance spectroscopy.
Anesthesiology. 2008 Sep; 109(3):426-35.A

Abstract

BACKGROUND

Antioxidant anesthetics such as propofol (2,6-diisopropylphenol) directly inhibit lipid peroxidation via the generation of reactive oxygen species. Currently, there are no other studies regarding the direct effects of propofol medium chain triglyceride/long chain triglyceride (MCT/LCT) on reactive oxygen species generation or in experimental models of reactive oxygen species-induced oxidative stress in the brain.

METHODS

The authors investigated the effects of propofol MCT/LCT on reactive oxygen species (hydroxyl radical or superoxide) by electron spin resonance spin trapping with 5,5-dimethyl-1-pyrroline-N-oxide. The effects of propofol MCT/LCT on oxidative stress in the brain of Wistar-Kyoto rats or stroke-prone spontaneously hypertensive rats were investigated by using an in vivo L-band electron spin resonance system to monitor the decay rate of 3-methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl as a nitroxyl spin probe.

RESULTS

These studies provided direct evidence that propofol MCT/LCT inhibited hydroxyl radical generation, but not superoxide generation. Regarding the hydroxyl radical from the Fenton system, it is likely to be due to the scavenging effects of vehicle. Anesthesia with propofol MCT/LCT reduced the degree of the high oxidative stress in the brain of stroke-prone spontaneously hypertensive rats.

CONCLUSION

The current data show that propofol, mixed with clinical reagents (propofol MCT/LCT), resulted in the down-regulation of high oxidative stress due to scavenging hydroxyl radical, as demonstrated by in vitro or in vivo electron spin resonance analysis. These results led to reduced levels of hydroxyl radical, formed by brain injury such as stroke, and may therefore provide advantages for neuroprotection during anesthesia for craniotomy, e.g., in cases of brain disease.

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Authors+Show Affiliations

Kobayashi K
Department of Clinical Care Medicine, Division of Anesthesiology, Kanagawa Dental College, Yokosuka, Kanagawa, Japan.
Yoshino F
No affiliation info available
Takahashi SS
No affiliation info available
Todoki K
No affiliation info available
Maehata Y
No affiliation info available
Komatsu T
No affiliation info available
Yoshida K
No affiliation info available
Lee MC
No affiliation info available

MeSH

Anesthetics, IntravenousAnimalsAntioxidantsBrainCerebrovascular CirculationDisease Models, AnimalElectron Spin Resonance SpectroscopyHydrogen PeroxideIronLipid PeroxidationMaleOxidative StressPropofolRatsRats, Inbred SHRRats, WistarReactive Oxygen SpeciesSuperoxidesTriglyceridesUltraviolet Rays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18719440

Citation

Kobayashi, Kyo, et al. "Direct Assessments of the Antioxidant Effects of Propofol Medium Chain Triglyceride/long Chain Triglyceride On the Brain of Stroke-prone Spontaneously Hypertensive Rats Using Electron Spin Resonance Spectroscopy." Anesthesiology, vol. 109, no. 3, 2008, pp. 426-35.
Kobayashi K, Yoshino F, Takahashi SS, et al. Direct assessments of the antioxidant effects of propofol medium chain triglyceride/long chain triglyceride on the brain of stroke-prone spontaneously hypertensive rats using electron spin resonance spectroscopy. Anesthesiology. 2008;109(3):426-35.
Kobayashi, K., Yoshino, F., Takahashi, S. S., Todoki, K., Maehata, Y., Komatsu, T., Yoshida, K., & Lee, M. C. (2008). Direct assessments of the antioxidant effects of propofol medium chain triglyceride/long chain triglyceride on the brain of stroke-prone spontaneously hypertensive rats using electron spin resonance spectroscopy. Anesthesiology, 109(3), 426-35. https://doi.org/10.1097/ALN.0b013e318182a903
Kobayashi K, et al. Direct Assessments of the Antioxidant Effects of Propofol Medium Chain Triglyceride/long Chain Triglyceride On the Brain of Stroke-prone Spontaneously Hypertensive Rats Using Electron Spin Resonance Spectroscopy. Anesthesiology. 2008;109(3):426-35. PubMed PMID: 18719440.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct assessments of the antioxidant effects of propofol medium chain triglyceride/long chain triglyceride on the brain of stroke-prone spontaneously hypertensive rats using electron spin resonance spectroscopy. AU - Kobayashi,Kyo, AU - Yoshino,Fumihiko, AU - Takahashi,Shun-Suke, AU - Todoki,Kazuo, AU - Maehata,Yojiro, AU - Komatsu,Tomoko, AU - Yoshida,Kazu-Ichi, AU - Lee,Masaichi-Chang-Il, PY - 2008/8/23/pubmed PY - 2008/10/1/medline PY - 2008/8/23/entrez SP - 426 EP - 35 JF - Anesthesiology JO - Anesthesiology VL - 109 IS - 3 N2 - BACKGROUND: Antioxidant anesthetics such as propofol (2,6-diisopropylphenol) directly inhibit lipid peroxidation via the generation of reactive oxygen species. Currently, there are no other studies regarding the direct effects of propofol medium chain triglyceride/long chain triglyceride (MCT/LCT) on reactive oxygen species generation or in experimental models of reactive oxygen species-induced oxidative stress in the brain. METHODS: The authors investigated the effects of propofol MCT/LCT on reactive oxygen species (hydroxyl radical or superoxide) by electron spin resonance spin trapping with 5,5-dimethyl-1-pyrroline-N-oxide. The effects of propofol MCT/LCT on oxidative stress in the brain of Wistar-Kyoto rats or stroke-prone spontaneously hypertensive rats were investigated by using an in vivo L-band electron spin resonance system to monitor the decay rate of 3-methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl as a nitroxyl spin probe. RESULTS: These studies provided direct evidence that propofol MCT/LCT inhibited hydroxyl radical generation, but not superoxide generation. Regarding the hydroxyl radical from the Fenton system, it is likely to be due to the scavenging effects of vehicle. Anesthesia with propofol MCT/LCT reduced the degree of the high oxidative stress in the brain of stroke-prone spontaneously hypertensive rats. CONCLUSION: The current data show that propofol, mixed with clinical reagents (propofol MCT/LCT), resulted in the down-regulation of high oxidative stress due to scavenging hydroxyl radical, as demonstrated by in vitro or in vivo electron spin resonance analysis. These results led to reduced levels of hydroxyl radical, formed by brain injury such as stroke, and may therefore provide advantages for neuroprotection during anesthesia for craniotomy, e.g., in cases of brain disease. SN - 1528-1175 UR - https://www.unboundmedicine.com/medline/citation/18719440/Direct_assessments_of_the_antioxidant_effects_of_propofol_medium_chain_triglyceride/long_chain_triglyceride_on_the_brain_of_stroke_prone_spontaneously_hypertensive_rats_using_electron_spin_resonance_spectroscopy_ L2 - https://pubs.asahq.org/anesthesiology/article-lookup/doi/10.1097/ALN.0b013e318182a903 DB - PRIME DP - Unbound Medicine ER -