Tags

Type your tag names separated by a space and hit enter

Applications of silver nanoparticles capped with different functional groups as the matrix and affinity probes in surface-assisted laser desorption/ionization time-of-flight and atmospheric pressure matrix-assisted laser desorption/ionization ion trap mass spectrometry for rapid analysis of sulfur drugs and biothiols in human urine.
Rapid Commun Mass Spectrom. 2008 Sep; 22(18):2863-72.RC

Abstract

A strategy is presented for the analysis of sulfur drugs and biothiols using silver nanoparticles (AgNPs) capped with different functional groups as the matrix and affinity probes in surface-assisted laser desorption/ionization time-of-flight mass spectrometry (SALDI-TOF MS) and atmospheric pressure-matrix assisted laser desorption/ionization ion trap mass spectrometry (AP-MALDI-ITMS). Biothiols adsorbed on the surface of AgNPs through covalent bonding were subjected to ultraviolet (UV) radiation that enabled desorption and ionization due to the excellent photochemical property of NPs. The proposed method has been successfully applied for the determination of cysteine and homocysteine in human urine samples using an internal standard. The limit of detection (LOD) and limit of quantification (LOQ) for cysteine and homocysteine in urine sample are 7 and 22 nM, respectively, with a relative standard deviation (RSD) of <10%. The advantages of the present method compared with the methods reported in the literature for biothiol analysis are simplicity, rapidity and sensitivity without the need for time-consuming separation and tedious preconcentration processes. Additionally, we also found that the bare AgNPs can be directly used as the matrix in MALDI-TOF MS for the analysis of sulfur drugs without the addition of an extra proton source.

Authors+Show Affiliations

Department of Chemistry, Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, Kaohsiung 804, Taiwan.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18720468

Citation

Shrivas, Kamlesh, and Hui-Fen Wu. "Applications of Silver Nanoparticles Capped With Different Functional Groups as the Matrix and Affinity Probes in Surface-assisted Laser Desorption/ionization Time-of-flight and Atmospheric Pressure Matrix-assisted Laser Desorption/ionization Ion Trap Mass Spectrometry for Rapid Analysis of Sulfur Drugs and Biothiols in Human Urine." Rapid Communications in Mass Spectrometry : RCM, vol. 22, no. 18, 2008, pp. 2863-72.
Shrivas K, Wu HF. Applications of silver nanoparticles capped with different functional groups as the matrix and affinity probes in surface-assisted laser desorption/ionization time-of-flight and atmospheric pressure matrix-assisted laser desorption/ionization ion trap mass spectrometry for rapid analysis of sulfur drugs and biothiols in human urine. Rapid Commun Mass Spectrom. 2008;22(18):2863-72.
Shrivas, K., & Wu, H. F. (2008). Applications of silver nanoparticles capped with different functional groups as the matrix and affinity probes in surface-assisted laser desorption/ionization time-of-flight and atmospheric pressure matrix-assisted laser desorption/ionization ion trap mass spectrometry for rapid analysis of sulfur drugs and biothiols in human urine. Rapid Communications in Mass Spectrometry : RCM, 22(18), 2863-72. https://doi.org/10.1002/rcm.3681
Shrivas K, Wu HF. Applications of Silver Nanoparticles Capped With Different Functional Groups as the Matrix and Affinity Probes in Surface-assisted Laser Desorption/ionization Time-of-flight and Atmospheric Pressure Matrix-assisted Laser Desorption/ionization Ion Trap Mass Spectrometry for Rapid Analysis of Sulfur Drugs and Biothiols in Human Urine. Rapid Commun Mass Spectrom. 2008;22(18):2863-72. PubMed PMID: 18720468.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Applications of silver nanoparticles capped with different functional groups as the matrix and affinity probes in surface-assisted laser desorption/ionization time-of-flight and atmospheric pressure matrix-assisted laser desorption/ionization ion trap mass spectrometry for rapid analysis of sulfur drugs and biothiols in human urine. AU - Shrivas,Kamlesh, AU - Wu,Hui-Fen, PY - 2008/8/23/pubmed PY - 2008/10/31/medline PY - 2008/8/23/entrez SP - 2863 EP - 72 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 22 IS - 18 N2 - A strategy is presented for the analysis of sulfur drugs and biothiols using silver nanoparticles (AgNPs) capped with different functional groups as the matrix and affinity probes in surface-assisted laser desorption/ionization time-of-flight mass spectrometry (SALDI-TOF MS) and atmospheric pressure-matrix assisted laser desorption/ionization ion trap mass spectrometry (AP-MALDI-ITMS). Biothiols adsorbed on the surface of AgNPs through covalent bonding were subjected to ultraviolet (UV) radiation that enabled desorption and ionization due to the excellent photochemical property of NPs. The proposed method has been successfully applied for the determination of cysteine and homocysteine in human urine samples using an internal standard. The limit of detection (LOD) and limit of quantification (LOQ) for cysteine and homocysteine in urine sample are 7 and 22 nM, respectively, with a relative standard deviation (RSD) of <10%. The advantages of the present method compared with the methods reported in the literature for biothiol analysis are simplicity, rapidity and sensitivity without the need for time-consuming separation and tedious preconcentration processes. Additionally, we also found that the bare AgNPs can be directly used as the matrix in MALDI-TOF MS for the analysis of sulfur drugs without the addition of an extra proton source. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/18720468/Applications_of_silver_nanoparticles_capped_with_different_functional_groups_as_the_matrix_and_affinity_probes_in_surface_assisted_laser_desorption/ionization_time_of_flight_and_atmospheric_pressure_matrix_assisted_laser_desorption/ionization_ion_trap_mass_spectrometry_for_rapid_analysis_of_sulfur_drugs_and_biothiols_in_human_urine_ L2 - https://doi.org/10.1002/rcm.3681 DB - PRIME DP - Unbound Medicine ER -