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Direct electrochemistry of hemoglobin on carbonized titania nanotubes and its application in a sensitive reagentless hydrogen peroxide biosensor.
Biosens Bioelectron. 2008 Dec 01; 24(4):825-30.BB

Abstract

Carbonized TiO(2) nanotubes (TNT/C) prepared by carbonization with organic polymers possess advantages combined from high conductivity of carbon and nanostructure of TiO(2) nanotubes. The material was used as a supporting matrix to immobilize a redox protein, hemoglobin (Hb), to explore its direct electron transfer ability. The apparent heterogeneous electron transfer rate constant (k(ET)) of Hb on TNT/C is 108s(-1), which is much higher than that in the reported works, demonstrating excellent direct electrochemistry behavior. The TNT/C-Hb modified glassy carbon electrode (GCE) demonstrates significant electrocatalytic activity for reduction of hydrogen peroxide with a small apparent Michaelis-Menten constant (87.5 microM). The TNT/C-Hb based H(2)O(2) sensor has a low detection limit (0.92 microM), fast response time (3s) and high dynamic response range (10(-6) to 10(-4)M), a much better performance than the reported works. These results demonstrate that a direct electrochemistry behavior can be significantly enhanced through simple carbon coating on a nanostructured material for higher reaction surface area and better conductivity. This work suggests that Hb-immobilized TNT/C has potential applications in a sensitive H(2)O(2) sensor.

Authors+Show Affiliations

School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637457, Singapore.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18722103

Citation

Guo, Chunxian, et al. "Direct Electrochemistry of Hemoglobin On Carbonized Titania Nanotubes and Its Application in a Sensitive Reagentless Hydrogen Peroxide Biosensor." Biosensors & Bioelectronics, vol. 24, no. 4, 2008, pp. 825-30.
Guo C, Hu F, Li CM, et al. Direct electrochemistry of hemoglobin on carbonized titania nanotubes and its application in a sensitive reagentless hydrogen peroxide biosensor. Biosens Bioelectron. 2008;24(4):825-30.
Guo, C., Hu, F., Li, C. M., & Shen, P. K. (2008). Direct electrochemistry of hemoglobin on carbonized titania nanotubes and its application in a sensitive reagentless hydrogen peroxide biosensor. Biosensors & Bioelectronics, 24(4), 825-30. https://doi.org/10.1016/j.bios.2008.07.007
Guo C, et al. Direct Electrochemistry of Hemoglobin On Carbonized Titania Nanotubes and Its Application in a Sensitive Reagentless Hydrogen Peroxide Biosensor. Biosens Bioelectron. 2008 Dec 1;24(4):825-30. PubMed PMID: 18722103.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct electrochemistry of hemoglobin on carbonized titania nanotubes and its application in a sensitive reagentless hydrogen peroxide biosensor. AU - Guo,Chunxian, AU - Hu,Fengping, AU - Li,Chang Ming, AU - Shen,Pei Kang, Y1 - 2008/07/15/ PY - 2008/04/09/received PY - 2008/07/02/revised PY - 2008/07/03/accepted PY - 2008/8/30/pubmed PY - 2009/2/13/medline PY - 2008/8/30/entrez SP - 825 EP - 30 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 24 IS - 4 N2 - Carbonized TiO(2) nanotubes (TNT/C) prepared by carbonization with organic polymers possess advantages combined from high conductivity of carbon and nanostructure of TiO(2) nanotubes. The material was used as a supporting matrix to immobilize a redox protein, hemoglobin (Hb), to explore its direct electron transfer ability. The apparent heterogeneous electron transfer rate constant (k(ET)) of Hb on TNT/C is 108s(-1), which is much higher than that in the reported works, demonstrating excellent direct electrochemistry behavior. The TNT/C-Hb modified glassy carbon electrode (GCE) demonstrates significant electrocatalytic activity for reduction of hydrogen peroxide with a small apparent Michaelis-Menten constant (87.5 microM). The TNT/C-Hb based H(2)O(2) sensor has a low detection limit (0.92 microM), fast response time (3s) and high dynamic response range (10(-6) to 10(-4)M), a much better performance than the reported works. These results demonstrate that a direct electrochemistry behavior can be significantly enhanced through simple carbon coating on a nanostructured material for higher reaction surface area and better conductivity. This work suggests that Hb-immobilized TNT/C has potential applications in a sensitive H(2)O(2) sensor. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/18722103/Direct_electrochemistry_of_hemoglobin_on_carbonized_titania_nanotubes_and_its_application_in_a_sensitive_reagentless_hydrogen_peroxide_biosensor_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(08)00341-2 DB - PRIME DP - Unbound Medicine ER -