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An indirect competitive immunoassay for insulin autoantibodies based on surface plasmon resonance.
Biosens Bioelectron. 2008 Dec 01; 24(4):882-7.BB

Abstract

We have developed a sensitive and specific method based on surface plasmon resonance (SPR) for detection of insulin autoantibodies (IAA) in serum samples from individuals at high risk of developing type 1 diabetes (T1D). When measuring trace molecules in undiluted sera with label-free techniques like SPR, non-specific adsorption of matrix proteins to the sensor surface is often a problem, since it causes a signal that masks the analyte response. The developed method is an indirect competitive immunoassay designed to overcome these problems. Today, IAA is mainly measured in radio immunoassays (RIAs), which are time consuming and require radioactively labeled antigen. With our SPR-based immunoassay the overall assay time is reduced by a factor of >100 (4 days to 50min), while sensitivity is maintained at a level comparable to that offered by RIA.

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Authors+Show Affiliations

Carlsson J
Division of Sensor Science and Molecular Physics, Department of Physics, Chemistry and Biology (IFM), Linköping University, SE-581 83 Linköping, Sweden.
Gullstrand C
No affiliation info available
Westermark GT
No affiliation info available
Ludvigsson J
No affiliation info available
Enander K
No affiliation info available
Liedberg B
No affiliation info available

MeSH

AutoantibodiesBiosensing TechniquesChildDiabetes Mellitus, Type 1Equipment DesignEquipment Failure AnalysisHumansImmunoassayInsulinReproducibility of ResultsSensitivity and SpecificitySurface Plasmon Resonance

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18722764

Citation

Carlsson, Jenny, et al. "An Indirect Competitive Immunoassay for Insulin Autoantibodies Based On Surface Plasmon Resonance." Biosensors & Bioelectronics, vol. 24, no. 4, 2008, pp. 882-7.
Carlsson J, Gullstrand C, Westermark GT, et al. An indirect competitive immunoassay for insulin autoantibodies based on surface plasmon resonance. Biosens Bioelectron. 2008;24(4):882-7.
Carlsson, J., Gullstrand, C., Westermark, G. T., Ludvigsson, J., Enander, K., & Liedberg, B. (2008). An indirect competitive immunoassay for insulin autoantibodies based on surface plasmon resonance. Biosensors & Bioelectronics, 24(4), 882-7. https://doi.org/10.1016/j.bios.2008.07.018
Carlsson J, et al. An Indirect Competitive Immunoassay for Insulin Autoantibodies Based On Surface Plasmon Resonance. Biosens Bioelectron. 2008 Dec 1;24(4):882-7. PubMed PMID: 18722764.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An indirect competitive immunoassay for insulin autoantibodies based on surface plasmon resonance. AU - Carlsson,Jenny, AU - Gullstrand,Camilla, AU - Westermark,Gunilla T, AU - Ludvigsson,Johnny, AU - Enander,Karin, AU - Liedberg,Bo, Y1 - 2008/07/19/ PY - 2008/05/14/received PY - 2008/06/27/revised PY - 2008/07/10/accepted PY - 2008/8/30/pubmed PY - 2009/2/13/medline PY - 2008/8/30/entrez SP - 882 EP - 7 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 24 IS - 4 N2 - We have developed a sensitive and specific method based on surface plasmon resonance (SPR) for detection of insulin autoantibodies (IAA) in serum samples from individuals at high risk of developing type 1 diabetes (T1D). When measuring trace molecules in undiluted sera with label-free techniques like SPR, non-specific adsorption of matrix proteins to the sensor surface is often a problem, since it causes a signal that masks the analyte response. The developed method is an indirect competitive immunoassay designed to overcome these problems. Today, IAA is mainly measured in radio immunoassays (RIAs), which are time consuming and require radioactively labeled antigen. With our SPR-based immunoassay the overall assay time is reduced by a factor of >100 (4 days to 50min), while sensitivity is maintained at a level comparable to that offered by RIA. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/18722764/An_indirect_competitive_immunoassay_for_insulin_autoantibodies_based_on_surface_plasmon_resonance_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(08)00361-8 DB - PRIME DP - Unbound Medicine ER -