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Protective effects of vitamin E and curcumin on L-thyroxine-induced rat testicular oxidative stress.
Chem Biol Interact. 2008 Nov 25; 176(2-3):121-8.CB

Abstract

Present study examines effects of curcumin and vitamin E on oxidative stress parameters, antioxidant defence enzymes and oxidized (GSSG) and reduced glutathione (GSH) levels in testis of L-thyroxine (T4)-induced hyperthyroid rats. The oxidative stress in T4-treated rat testis was evident from elevation in oxidative stress parameters such as lipid peroxide and protein carbonyl contents, decrease in superoxide dismutase (SOD) and catalase (CAT) activities and increase in glutathione peroxidase (GPx) activity. This is accompanied with decrease in number and mortality of epididymal sperms. When the T4-treated rats were fed with vitamin E and/or curcumin, the lipid peroxide and protein carbonyl contents in crude homogenates of testes decreased to normal level. Treatment of curcumin and/or vitamin E to T4-treated rats resulted in elevation of SOD level in postmitochondrial fraction (PMF) and mitochondrial fraction (MF) and CAT in PMF, with decreased GPx activity in MF. However, curcumin or vitamin E was unable to change GPx activity alone but in together they elevated the GPx in PMF of T4-treated rat testis. Both the antioxidants are incapable of producing significant changes in GSH:GSSG ratio of PMF of T4-treated rats. In MF, GSH:GSSG ratio elevated and decreased respectively by curcumin and vitamin E treatments to T4-treated rats, however, in together these antioxidants caused an elevated GSH:GSSG ratio with a value less than when vitamin E given alone to T4-treated rats. Vitamin E not the curcumin elevates total sperm count and percentage of live sperm impaired by hyperthyroid state. In summary, both vitamin E and curcumin are efficient in protecting testis from oxidative stress generated by T4 mainly in restoring antioxidant enzymes to the level of euthyroid animals up to some extent but vitamin E is more efficient than curcumin.

Authors+Show Affiliations

Department of Biotechnology and Zoology, Utkal University, Bhubaneswar 751004, Orissa, India.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18723006

Citation

Sahoo, Dipak Kumar, et al. "Protective Effects of Vitamin E and Curcumin On L-thyroxine-induced Rat Testicular Oxidative Stress." Chemico-biological Interactions, vol. 176, no. 2-3, 2008, pp. 121-8.
Sahoo DK, Roy A, Chainy GB. Protective effects of vitamin E and curcumin on L-thyroxine-induced rat testicular oxidative stress. Chem Biol Interact. 2008;176(2-3):121-8.
Sahoo, D. K., Roy, A., & Chainy, G. B. (2008). Protective effects of vitamin E and curcumin on L-thyroxine-induced rat testicular oxidative stress. Chemico-biological Interactions, 176(2-3), 121-8. https://doi.org/10.1016/j.cbi.2008.07.009
Sahoo DK, Roy A, Chainy GB. Protective Effects of Vitamin E and Curcumin On L-thyroxine-induced Rat Testicular Oxidative Stress. Chem Biol Interact. 2008 Nov 25;176(2-3):121-8. PubMed PMID: 18723006.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of vitamin E and curcumin on L-thyroxine-induced rat testicular oxidative stress. AU - Sahoo,Dipak Kumar, AU - Roy,Anita, AU - Chainy,Gagan B N, Y1 - 2008/08/03/ PY - 2008/04/02/received PY - 2008/07/23/revised PY - 2008/07/23/accepted PY - 2008/8/30/pubmed PY - 2009/1/22/medline PY - 2008/8/30/entrez SP - 121 EP - 8 JF - Chemico-biological interactions JO - Chem. Biol. Interact. VL - 176 IS - 2-3 N2 - Present study examines effects of curcumin and vitamin E on oxidative stress parameters, antioxidant defence enzymes and oxidized (GSSG) and reduced glutathione (GSH) levels in testis of L-thyroxine (T4)-induced hyperthyroid rats. The oxidative stress in T4-treated rat testis was evident from elevation in oxidative stress parameters such as lipid peroxide and protein carbonyl contents, decrease in superoxide dismutase (SOD) and catalase (CAT) activities and increase in glutathione peroxidase (GPx) activity. This is accompanied with decrease in number and mortality of epididymal sperms. When the T4-treated rats were fed with vitamin E and/or curcumin, the lipid peroxide and protein carbonyl contents in crude homogenates of testes decreased to normal level. Treatment of curcumin and/or vitamin E to T4-treated rats resulted in elevation of SOD level in postmitochondrial fraction (PMF) and mitochondrial fraction (MF) and CAT in PMF, with decreased GPx activity in MF. However, curcumin or vitamin E was unable to change GPx activity alone but in together they elevated the GPx in PMF of T4-treated rat testis. Both the antioxidants are incapable of producing significant changes in GSH:GSSG ratio of PMF of T4-treated rats. In MF, GSH:GSSG ratio elevated and decreased respectively by curcumin and vitamin E treatments to T4-treated rats, however, in together these antioxidants caused an elevated GSH:GSSG ratio with a value less than when vitamin E given alone to T4-treated rats. Vitamin E not the curcumin elevates total sperm count and percentage of live sperm impaired by hyperthyroid state. In summary, both vitamin E and curcumin are efficient in protecting testis from oxidative stress generated by T4 mainly in restoring antioxidant enzymes to the level of euthyroid animals up to some extent but vitamin E is more efficient than curcumin. SN - 1872-7786 UR - https://www.unboundmedicine.com/medline/citation/18723006/Protective_effects_of_vitamin_E_and_curcumin_on_L_thyroxine_induced_rat_testicular_oxidative_stress_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-2797(08)00374-8 DB - PRIME DP - Unbound Medicine ER -