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Molecular and ultrastructural characterization of endothelial cells differentiated from human bone marrow mesenchymal stem cells.
Cell Biol Int. 2008 Oct; 32(10):1183-92.CB

Abstract

In this study characterization of endothelial cells differentiated from human bone marrow mesenchymal stem cells (hBMCs) was investigated in relation to their capillary network formation potential. Differentiation was performed in presence of vascular endothelial growth factor (VEGF) and insulin like growth factor-1 (IGF-1). A panel of cellular and molecular markers was used for characterization of the endothelial cells. The cells were strongly positive for von Willebrand factor (vWF) and vascular endothelial growth factor receptor 2 (VEGFR2) when measured at protein and mRNA levels. Development of endothelial cells was found to be associated with formation of typical organelles such as Weibel Palade (WP) bodies, Cavealae and pinocytic vesicles. Early vessel growth was also evidenced by showing specific junctions between the cells. The migratory and angiogenic properties of the cells were confirmed by showing capillary network formation in vitro. These results indicate that the capacity of endothelial cells differentiated from hBMSCs in formation of vascular system is consistent with molecular and structural development.

Authors+Show Affiliations

Department of Clinical Biochemistry, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18723100

Citation

Jazayeri, Maryam, et al. "Molecular and Ultrastructural Characterization of Endothelial Cells Differentiated From Human Bone Marrow Mesenchymal Stem Cells." Cell Biology International, vol. 32, no. 10, 2008, pp. 1183-92.
Jazayeri M, Allameh A, Soleimani M, et al. Molecular and ultrastructural characterization of endothelial cells differentiated from human bone marrow mesenchymal stem cells. Cell Biol Int. 2008;32(10):1183-92.
Jazayeri, M., Allameh, A., Soleimani, M., Jazayeri, S. H., Piryaei, A., & Kazemnejad, S. (2008). Molecular and ultrastructural characterization of endothelial cells differentiated from human bone marrow mesenchymal stem cells. Cell Biology International, 32(10), 1183-92. https://doi.org/10.1016/j.cellbi.2008.07.020
Jazayeri M, et al. Molecular and Ultrastructural Characterization of Endothelial Cells Differentiated From Human Bone Marrow Mesenchymal Stem Cells. Cell Biol Int. 2008;32(10):1183-92. PubMed PMID: 18723100.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular and ultrastructural characterization of endothelial cells differentiated from human bone marrow mesenchymal stem cells. AU - Jazayeri,Maryam, AU - Allameh,Abdolamir, AU - Soleimani,Masoud, AU - Jazayeri,Seyed Hamid, AU - Piryaei,Abbas, AU - Kazemnejad,Somaieh, Y1 - 2008/08/06/ PY - 2007/12/10/received PY - 2008/04/20/revised PY - 2008/07/30/accepted PY - 2008/8/30/pubmed PY - 2008/12/23/medline PY - 2008/8/30/entrez SP - 1183 EP - 92 JF - Cell biology international JO - Cell Biol. Int. VL - 32 IS - 10 N2 - In this study characterization of endothelial cells differentiated from human bone marrow mesenchymal stem cells (hBMCs) was investigated in relation to their capillary network formation potential. Differentiation was performed in presence of vascular endothelial growth factor (VEGF) and insulin like growth factor-1 (IGF-1). A panel of cellular and molecular markers was used for characterization of the endothelial cells. The cells were strongly positive for von Willebrand factor (vWF) and vascular endothelial growth factor receptor 2 (VEGFR2) when measured at protein and mRNA levels. Development of endothelial cells was found to be associated with formation of typical organelles such as Weibel Palade (WP) bodies, Cavealae and pinocytic vesicles. Early vessel growth was also evidenced by showing specific junctions between the cells. The migratory and angiogenic properties of the cells were confirmed by showing capillary network formation in vitro. These results indicate that the capacity of endothelial cells differentiated from hBMSCs in formation of vascular system is consistent with molecular and structural development. SN - 1065-6995 UR - https://www.unboundmedicine.com/medline/citation/18723100/Molecular_and_ultrastructural_characterization_of_endothelial_cells_differentiated_from_human_bone_marrow_mesenchymal_stem_cells_ DB - PRIME DP - Unbound Medicine ER -