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Insulinotropic effect of aqueous extract of Brassica nigra improves glucose homeostasis in streptozotocin induced diabetic rats.
Exp Clin Endocrinol Diabetes. 2009 Jun; 117(6):251-6.EC

Abstract

Aqueous extract of BRASSICA NIGRA (AEBN) has been shown to have good antidiabetic effect along with significant decrease (p<0.01) of abnormal serum lipid levels in our previous study. To understand the mechanism of action, effect of oral administration of AEBN for two months on glycolytic and gluconeogenic enzymes was studied in liver and kidney tissues of rats with streptozotocin (STZ) induced diabetes mellitus. The activities of gluconeogenic enzymes were higher and of glycolytic enzymes were decreased in both the liver and kidney tissues during diabetes. However, in diabetic rats treated with AEBN for two months, decrease of serum glucose, increase of serum insulin and release of insulin from pancreas (shown in vitro from isolated pancreas) along with the restoration of key regulatory enzyme activities of carbohydrate metabolism and glycogen content were observed. The therapeutic role of AEBN in STZ induced diabetes as exemplified in this study can be attributed to the release of insulin from pancreas and change of glucose metabolizing enzyme activities to normal levels, thus stabilizing glucose homeostasis in the liver and kidney. The LD50 was found to be more than 15 times the effective dose (ED) implying higher margin of safety for AEBN. These biochemical effects indicate that AEBN could be a possible new therapeutic agent for the treatment of diabetes.

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Authors+Show Affiliations

Anand P
Dr. B. R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi, India.
Murali YK
No affiliation info available
Tandon V
No affiliation info available
Murthy PS
No affiliation info available
Chandra R
No affiliation info available

MeSH

AnimalsCarbohydrate MetabolismDiabetes Mellitus, ExperimentalDrug Evaluation, PreclinicalGluconeogenesisGlucoseGlycolysisHomeostasisHypoglycemic AgentsInsulinKidneyLipid MetabolismLiverMaleMustard PlantPancreasPlant ExtractsRatsRats, WistarTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18726874

Citation

Anand, P, et al. "Insulinotropic Effect of Aqueous Extract of Brassica Nigra Improves Glucose Homeostasis in Streptozotocin Induced Diabetic Rats." Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [and] German Diabetes Association, vol. 117, no. 6, 2009, pp. 251-6.
Anand P, Murali YK, Tandon V, et al. Insulinotropic effect of aqueous extract of Brassica nigra improves glucose homeostasis in streptozotocin induced diabetic rats. Exp Clin Endocrinol Diabetes. 2009;117(6):251-6.
Anand, P., Murali, Y. K., Tandon, V., Murthy, P. S., & Chandra, R. (2009). Insulinotropic effect of aqueous extract of Brassica nigra improves glucose homeostasis in streptozotocin induced diabetic rats. Experimental and Clinical Endocrinology & Diabetes : Official Journal, German Society of Endocrinology [and] German Diabetes Association, 117(6), 251-6. https://doi.org/10.1055/s-2008-1080917
Anand P, et al. Insulinotropic Effect of Aqueous Extract of Brassica Nigra Improves Glucose Homeostasis in Streptozotocin Induced Diabetic Rats. Exp Clin Endocrinol Diabetes. 2009;117(6):251-6. PubMed PMID: 18726874.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Insulinotropic effect of aqueous extract of Brassica nigra improves glucose homeostasis in streptozotocin induced diabetic rats. AU - Anand,P, AU - Murali,Y K, AU - Tandon,V, AU - Murthy,P S, AU - Chandra,R, Y1 - 2008/08/25/ PY - 2008/8/30/pubmed PY - 2009/8/19/medline PY - 2008/8/30/entrez SP - 251 EP - 6 JF - Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association JO - Exp Clin Endocrinol Diabetes VL - 117 IS - 6 N2 - Aqueous extract of BRASSICA NIGRA (AEBN) has been shown to have good antidiabetic effect along with significant decrease (p<0.01) of abnormal serum lipid levels in our previous study. To understand the mechanism of action, effect of oral administration of AEBN for two months on glycolytic and gluconeogenic enzymes was studied in liver and kidney tissues of rats with streptozotocin (STZ) induced diabetes mellitus. The activities of gluconeogenic enzymes were higher and of glycolytic enzymes were decreased in both the liver and kidney tissues during diabetes. However, in diabetic rats treated with AEBN for two months, decrease of serum glucose, increase of serum insulin and release of insulin from pancreas (shown in vitro from isolated pancreas) along with the restoration of key regulatory enzyme activities of carbohydrate metabolism and glycogen content were observed. The therapeutic role of AEBN in STZ induced diabetes as exemplified in this study can be attributed to the release of insulin from pancreas and change of glucose metabolizing enzyme activities to normal levels, thus stabilizing glucose homeostasis in the liver and kidney. The LD50 was found to be more than 15 times the effective dose (ED) implying higher margin of safety for AEBN. These biochemical effects indicate that AEBN could be a possible new therapeutic agent for the treatment of diabetes. SN - 1439-3646 UR - https://www.unboundmedicine.com/medline/citation/18726874/Insulinotropic_effect_of_aqueous_extract_of_Brassica_nigra_improves_glucose_homeostasis_in_streptozotocin_induced_diabetic_rats_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-2008-1080917 DB - PRIME DP - Unbound Medicine ER -