Tags

Type your tag names separated by a space and hit enter

Potential therapy of Fc-antigen combination-encoding DNA vaccination in mouse allergic airway inflammation.
Clin Exp Immunol. 2008 Oct; 154(1):115-22.CE

Abstract

Vaccination with allergen-encoding DNA has been proposed as having potential for allergen-specific immunotherapy. In this study, we examine the therapeutic effect of allergen-encoding DNA vaccination directly to dendritic cells (DCs) on allergen-induced allergic airway inflammation in a mouse model and explore potential mechanism. Ovalbumin (OVA)-sensitized and challenged mice were immunized with DNA vaccine and received bronchoalveolar lavage (BAL) 1 day after the last challenge, to measure BAL levels of interleukin (IL)-4, IL-5, interferon (IFN)-gamma and differential cell count. Pulmonary DCs and Spleen DCs were purified and sorted according to the expression of CD(11c) (+)CD(80) (+) and CD(11c) (+)CD(86) (+) co-stimulatory molecules. Our data demonstrated that DNA vaccine therapy with OVA-Fc-pcDNA(3.1) significantly prevented OVA-increased levels of IL-4, IL-5 and the percentage of eosinophils and OVA-decreased level of IFN-gamma. OVA-Fc-pcDNA(3.1)-treated mice had less severity of airway inflammation, and lower expression of CD(11c) (+)CD(80) (+) and CD(11c) (+)CD(86) (+) on pulmonary DCs, as compared with animals with OVA-pcDNA(3.1,) pcDNA(3.1) and OVA respectively. DNA vaccine encoding both Fc and OVA was shown to be more effective than DNA vaccine encoding OVA alone. Our data indicate that Fc-antigen combination-encoding DNA vaccination has better preventive effects on antigen-induced airway inflammation by regulating DCs, and may be a new alternative therapy for asthma.

Authors+Show Affiliations

Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18727625

Citation

Wang, Y, et al. "Potential Therapy of Fc-antigen Combination-encoding DNA Vaccination in Mouse Allergic Airway Inflammation." Clinical and Experimental Immunology, vol. 154, no. 1, 2008, pp. 115-22.
Wang Y, Qian G, Wang G, et al. Potential therapy of Fc-antigen combination-encoding DNA vaccination in mouse allergic airway inflammation. Clin Exp Immunol. 2008;154(1):115-22.
Wang, Y., Qian, G., Wang, G., Cheng, X., Bai, C., & Wang, X. (2008). Potential therapy of Fc-antigen combination-encoding DNA vaccination in mouse allergic airway inflammation. Clinical and Experimental Immunology, 154(1), 115-22. https://doi.org/10.1111/j.1365-2249.2008.03736.x
Wang Y, et al. Potential Therapy of Fc-antigen Combination-encoding DNA Vaccination in Mouse Allergic Airway Inflammation. Clin Exp Immunol. 2008;154(1):115-22. PubMed PMID: 18727625.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Potential therapy of Fc-antigen combination-encoding DNA vaccination in mouse allergic airway inflammation. AU - Wang,Y, AU - Qian,G, AU - Wang,G, AU - Cheng,X, AU - Bai,C, AU - Wang,X, Y1 - 2008/08/22/ PY - 2008/8/30/pubmed PY - 2008/12/17/medline PY - 2008/8/30/entrez SP - 115 EP - 22 JF - Clinical and experimental immunology JO - Clin Exp Immunol VL - 154 IS - 1 N2 - Vaccination with allergen-encoding DNA has been proposed as having potential for allergen-specific immunotherapy. In this study, we examine the therapeutic effect of allergen-encoding DNA vaccination directly to dendritic cells (DCs) on allergen-induced allergic airway inflammation in a mouse model and explore potential mechanism. Ovalbumin (OVA)-sensitized and challenged mice were immunized with DNA vaccine and received bronchoalveolar lavage (BAL) 1 day after the last challenge, to measure BAL levels of interleukin (IL)-4, IL-5, interferon (IFN)-gamma and differential cell count. Pulmonary DCs and Spleen DCs were purified and sorted according to the expression of CD(11c) (+)CD(80) (+) and CD(11c) (+)CD(86) (+) co-stimulatory molecules. Our data demonstrated that DNA vaccine therapy with OVA-Fc-pcDNA(3.1) significantly prevented OVA-increased levels of IL-4, IL-5 and the percentage of eosinophils and OVA-decreased level of IFN-gamma. OVA-Fc-pcDNA(3.1)-treated mice had less severity of airway inflammation, and lower expression of CD(11c) (+)CD(80) (+) and CD(11c) (+)CD(86) (+) on pulmonary DCs, as compared with animals with OVA-pcDNA(3.1,) pcDNA(3.1) and OVA respectively. DNA vaccine encoding both Fc and OVA was shown to be more effective than DNA vaccine encoding OVA alone. Our data indicate that Fc-antigen combination-encoding DNA vaccination has better preventive effects on antigen-induced airway inflammation by regulating DCs, and may be a new alternative therapy for asthma. SN - 1365-2249 UR - https://www.unboundmedicine.com/medline/citation/18727625/Potential_therapy_of_Fc_antigen_combination_encoding_DNA_vaccination_in_mouse_allergic_airway_inflammation_ L2 - https://doi.org/10.1111/j.1365-2249.2008.03736.x DB - PRIME DP - Unbound Medicine ER -