Tags

Type your tag names separated by a space and hit enter

Effect of varicocele on chromatin condensation and DNA integrity of ejaculated spermatozoa using cytochemical tests.
Andrologia. 2008 Aug; 40(4):245-51.A

Abstract

Varicocele occurs in approximately 15% to 20% of the general male population and it is the most common cause of poor semen production and decreased semen quality. It has been demonstrated that patients with varicocele have a significantly higher DNA fragmentation index (DFI) and spermatozoa with nuclear anomalies than healthy fertile men. Therefore, the aim of this study was to evaluate sperm chromatin integrity in these patients. Sixty men referring to the andrology laboratory were categorised into three different groups: 20 infertile men with varicocele, 20 infertile men with abnormal semen parameters and 20 fertile men who had normal spermatogram were considered as control group. Semen analysis was performed according to WHO criteria. To evaluate sperm chromatin quality and DNA integrity, after fixation of sperm smears, aniline blue, toluidine blue, chromomycin A(3) and acridine orange staining were applied in three groups. The slides were analysed by light and fluorescent microscopy and to determine the percentage of mature or immature spermatozoa, 200 spermatozoa were counted in each slide. The results showed that the rates of aniline blue-reacted spermatozoa were significantly higher in infertile and varicocele patients than in the normal group (P < 0.001). In addition, with regard to chromomycin A(3), acridine orange and toluidine blue staining, there was a significant difference between the three groups (P < 0.001). The results showed that the varicocele samples contain a higher proportion of spermatozoa with abnormal DNA and immature chromatin than those from fertile men as well as infertile men without varicocele. Therefore, varicocele results in the production of spermatozoa with less condensed chromatin and this is one of the possible causes of infertility due to varicocele.

Authors+Show Affiliations

Research and Clinical Center for Infertility and Department of Anatomy, Yazd Shahid Sadoughi University of Medical Sciences, Yazd, Iran. prof_talebi@ssu.ac.irNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18727735

Citation

Talebi, A R., et al. "Effect of Varicocele On Chromatin Condensation and DNA Integrity of Ejaculated Spermatozoa Using Cytochemical Tests." Andrologia, vol. 40, no. 4, 2008, pp. 245-51.
Talebi AR, Moein MR, Tabibnejad N, et al. Effect of varicocele on chromatin condensation and DNA integrity of ejaculated spermatozoa using cytochemical tests. Andrologia. 2008;40(4):245-51.
Talebi, A. R., Moein, M. R., Tabibnejad, N., & Ghasemzadeh, J. (2008). Effect of varicocele on chromatin condensation and DNA integrity of ejaculated spermatozoa using cytochemical tests. Andrologia, 40(4), 245-51. https://doi.org/10.1111/j.1439-0272.2008.00852.x
Talebi AR, et al. Effect of Varicocele On Chromatin Condensation and DNA Integrity of Ejaculated Spermatozoa Using Cytochemical Tests. Andrologia. 2008;40(4):245-51. PubMed PMID: 18727735.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of varicocele on chromatin condensation and DNA integrity of ejaculated spermatozoa using cytochemical tests. AU - Talebi,A R, AU - Moein,M R, AU - Tabibnejad,N, AU - Ghasemzadeh,J, PY - 2008/8/30/pubmed PY - 2008/10/16/medline PY - 2008/8/30/entrez SP - 245 EP - 51 JF - Andrologia JO - Andrologia VL - 40 IS - 4 N2 - Varicocele occurs in approximately 15% to 20% of the general male population and it is the most common cause of poor semen production and decreased semen quality. It has been demonstrated that patients with varicocele have a significantly higher DNA fragmentation index (DFI) and spermatozoa with nuclear anomalies than healthy fertile men. Therefore, the aim of this study was to evaluate sperm chromatin integrity in these patients. Sixty men referring to the andrology laboratory were categorised into three different groups: 20 infertile men with varicocele, 20 infertile men with abnormal semen parameters and 20 fertile men who had normal spermatogram were considered as control group. Semen analysis was performed according to WHO criteria. To evaluate sperm chromatin quality and DNA integrity, after fixation of sperm smears, aniline blue, toluidine blue, chromomycin A(3) and acridine orange staining were applied in three groups. The slides were analysed by light and fluorescent microscopy and to determine the percentage of mature or immature spermatozoa, 200 spermatozoa were counted in each slide. The results showed that the rates of aniline blue-reacted spermatozoa were significantly higher in infertile and varicocele patients than in the normal group (P < 0.001). In addition, with regard to chromomycin A(3), acridine orange and toluidine blue staining, there was a significant difference between the three groups (P < 0.001). The results showed that the varicocele samples contain a higher proportion of spermatozoa with abnormal DNA and immature chromatin than those from fertile men as well as infertile men without varicocele. Therefore, varicocele results in the production of spermatozoa with less condensed chromatin and this is one of the possible causes of infertility due to varicocele. SN - 1439-0272 UR - https://www.unboundmedicine.com/medline/citation/18727735/Effect_of_varicocele_on_chromatin_condensation_and_DNA_integrity_of_ejaculated_spermatozoa_using_cytochemical_tests_ L2 - https://doi.org/10.1111/j.1439-0272.2008.00852.x DB - PRIME DP - Unbound Medicine ER -