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Strain construction for ethanol production from dilute-acid lignocellulosic hydrolysate.
Appl Biochem Biotechnol. 2009 Jun; 157(3):473-82.AB

Abstract

In order to construct a strain that converts sugar mixture and resist/metabolize inhibitors in lignocellulosic dilute-acid hydrolysate, the biotechnology of inactive intergeneric fusion between Saccharomyces cerevisiae and Pachysolen tannophilis was performed. Fusant 1 was successfully obtained as a hybrid strain, which was screened out by xylose and mixed sugar (xylose and glucose) fermentation. This strain showed good abilities of ethanol production, ethanol tolerance, and resistance to the toxic inhibitors presenting in the hydrolysate. The maximum volumetric yield of ethanol and yield of xylitol in mixed sugar was 9.52 g/l and 0.44 g/g, respectively. The results indicated that the constructed strain Fusant 1 was a good producer for ethanol and xylitol from lignocellulosic dilute-acid hydrolysate.

Authors+Show Affiliations

College of Life Science, Capital Normal University, No 105 North Street of West Third Ring Road, Beijing, 100048, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18751961

Citation

Yan, Fei, et al. "Strain Construction for Ethanol Production From Dilute-acid Lignocellulosic Hydrolysate." Applied Biochemistry and Biotechnology, vol. 157, no. 3, 2009, pp. 473-82.
Yan F, Bai F, Tian S, et al. Strain construction for ethanol production from dilute-acid lignocellulosic hydrolysate. Appl Biochem Biotechnol. 2009;157(3):473-82.
Yan, F., Bai, F., Tian, S., Zhang, J., Zhang, Z., & Yang, X. (2009). Strain construction for ethanol production from dilute-acid lignocellulosic hydrolysate. Applied Biochemistry and Biotechnology, 157(3), 473-82. https://doi.org/10.1007/s12010-008-8343-8
Yan F, et al. Strain Construction for Ethanol Production From Dilute-acid Lignocellulosic Hydrolysate. Appl Biochem Biotechnol. 2009;157(3):473-82. PubMed PMID: 18751961.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Strain construction for ethanol production from dilute-acid lignocellulosic hydrolysate. AU - Yan,Fei, AU - Bai,Fali, AU - Tian,Shen, AU - Zhang,Jinxin, AU - Zhang,Zuoyang, AU - Yang,Xiushan, Y1 - 2008/08/28/ PY - 2008/03/16/received PY - 2008/08/11/accepted PY - 2008/8/30/pubmed PY - 2009/7/3/medline PY - 2008/8/30/entrez SP - 473 EP - 82 JF - Applied biochemistry and biotechnology JO - Appl Biochem Biotechnol VL - 157 IS - 3 N2 - In order to construct a strain that converts sugar mixture and resist/metabolize inhibitors in lignocellulosic dilute-acid hydrolysate, the biotechnology of inactive intergeneric fusion between Saccharomyces cerevisiae and Pachysolen tannophilis was performed. Fusant 1 was successfully obtained as a hybrid strain, which was screened out by xylose and mixed sugar (xylose and glucose) fermentation. This strain showed good abilities of ethanol production, ethanol tolerance, and resistance to the toxic inhibitors presenting in the hydrolysate. The maximum volumetric yield of ethanol and yield of xylitol in mixed sugar was 9.52 g/l and 0.44 g/g, respectively. The results indicated that the constructed strain Fusant 1 was a good producer for ethanol and xylitol from lignocellulosic dilute-acid hydrolysate. SN - 1559-0291 UR - https://www.unboundmedicine.com/medline/citation/18751961/Strain_construction_for_ethanol_production_from_dilute_acid_lignocellulosic_hydrolysate_ L2 - https://dx.doi.org/10.1007/s12010-008-8343-8 DB - PRIME DP - Unbound Medicine ER -