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Staphylococcal cassette chromosome mec (SCCmec) characterization and panton-valentine leukocidin gene occurrence for methicillin-resistant Staphylococcus aureus in Turkey, from 2003 to 2006.
Antonie Van Leeuwenhoek. 2008 Nov; 94(4):607-14.AV

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) cause serious community-acquired and nosocomial diseases all over the world. We determined the SCCmec types and occurrence of the PVL gene by using TaqMan real-time PCR method, and correlated these with phenotypic antibiotic susceptibility patterns for MRSA strains collected from Gulhane Military Medical Academy Hospital (GMMAH) during 4 years study period. To our knowledge, this is the first report from Turkey of molecular SCCmec typing analysis of MRSA stains. A total of 385 clinical MRSA isolates collected in the clinical and Microbiology Laboratory at GMMAH between 2003 and 2006 were included in the study. Overall, SCCmec types-I, II, III, IV, V, nontypeable and PVL occurrence were detected in 11 (2.8%), 3 (0.8%), 316 (82.1%), 20 (5.1%), 20 (5.1%), 15 (3.9%) and 5 (1.3%) isolates, respectively. A total of 330 (85.5%) were SCCmec-I/II/III and 40 (10.3%) were SCCmec IV/V. SCCmec-I/II/III isolates were recovered more from patients with serious infections in surgical departments especially those with intensive care units than the SCCmec-IV/V isolates (chi(2) = 13.560, P < 0.001). SCCmec-I/II/III MRSA strains were predominantly recovered from blood stream (53.0%, P = 0.014), while SCCmec-IV/V strains were predominately isolated from skin and soft tissue and abscess (55.0%, P < 0.001). The PVL gene was detected in 10.0% of SCCmec-IV/V isolates in contrast to 0.3% in SCCmec-I/II/III (chi(2) = 25.164, P < 0.001). SCCmec-I/II/III MRSA strains were more resistant to clindamycin (chi(2) = 5.078, P = 0.024), amoxicillin-clavulanate (chi(2) = 84.912, P < 0.001), erythromycin (chi(2) = 4.651, P = 0.031), gentamicin (chi(2) = 24.869, P < 0.001), and rifampin (chi(2) = 18.878, P < 0.001) than SCCmec-IV/V MRSA strains. This data indicates that SCCmec-III MRSA strains that do not carry the PVL gene are the predominant MRSA strains in our hospital setting in Ankara, capital of Turkey and that SCCmec-I/II/III MRSA strains may cause serious infections in surgical departments especially those with intensive care units.

Authors+Show Affiliations

Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy and School of Medicine, 06018, Etlik, Ankara, Turkey. abkilic@gata.edu.trNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18752036

Citation

Kilic, Abdullah, et al. "Staphylococcal Cassette Chromosome Mec (SCCmec) Characterization and Panton-valentine Leukocidin Gene Occurrence for Methicillin-resistant Staphylococcus Aureus in Turkey, From 2003 to 2006." Antonie Van Leeuwenhoek, vol. 94, no. 4, 2008, pp. 607-14.
Kilic A, Guclu AU, Senses Z, et al. Staphylococcal cassette chromosome mec (SCCmec) characterization and panton-valentine leukocidin gene occurrence for methicillin-resistant Staphylococcus aureus in Turkey, from 2003 to 2006. Antonie Van Leeuwenhoek. 2008;94(4):607-14.
Kilic, A., Guclu, A. U., Senses, Z., Bedir, O., Aydogan, H., & Basustaoglu, A. C. (2008). Staphylococcal cassette chromosome mec (SCCmec) characterization and panton-valentine leukocidin gene occurrence for methicillin-resistant Staphylococcus aureus in Turkey, from 2003 to 2006. Antonie Van Leeuwenhoek, 94(4), 607-14. https://doi.org/10.1007/s10482-008-9278-3
Kilic A, et al. Staphylococcal Cassette Chromosome Mec (SCCmec) Characterization and Panton-valentine Leukocidin Gene Occurrence for Methicillin-resistant Staphylococcus Aureus in Turkey, From 2003 to 2006. Antonie Van Leeuwenhoek. 2008;94(4):607-14. PubMed PMID: 18752036.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Staphylococcal cassette chromosome mec (SCCmec) characterization and panton-valentine leukocidin gene occurrence for methicillin-resistant Staphylococcus aureus in Turkey, from 2003 to 2006. AU - Kilic,Abdullah, AU - Guclu,Aylin Uskudar, AU - Senses,Zeynep, AU - Bedir,Orhan, AU - Aydogan,Hakan, AU - Basustaoglu,A Celal, Y1 - 2008/08/28/ PY - 2008/07/22/received PY - 2008/08/18/accepted PY - 2008/8/30/pubmed PY - 2008/11/14/medline PY - 2008/8/30/entrez SP - 607 EP - 14 JF - Antonie van Leeuwenhoek JO - Antonie Van Leeuwenhoek VL - 94 IS - 4 N2 - Methicillin-resistant Staphylococcus aureus (MRSA) cause serious community-acquired and nosocomial diseases all over the world. We determined the SCCmec types and occurrence of the PVL gene by using TaqMan real-time PCR method, and correlated these with phenotypic antibiotic susceptibility patterns for MRSA strains collected from Gulhane Military Medical Academy Hospital (GMMAH) during 4 years study period. To our knowledge, this is the first report from Turkey of molecular SCCmec typing analysis of MRSA stains. A total of 385 clinical MRSA isolates collected in the clinical and Microbiology Laboratory at GMMAH between 2003 and 2006 were included in the study. Overall, SCCmec types-I, II, III, IV, V, nontypeable and PVL occurrence were detected in 11 (2.8%), 3 (0.8%), 316 (82.1%), 20 (5.1%), 20 (5.1%), 15 (3.9%) and 5 (1.3%) isolates, respectively. A total of 330 (85.5%) were SCCmec-I/II/III and 40 (10.3%) were SCCmec IV/V. SCCmec-I/II/III isolates were recovered more from patients with serious infections in surgical departments especially those with intensive care units than the SCCmec-IV/V isolates (chi(2) = 13.560, P < 0.001). SCCmec-I/II/III MRSA strains were predominantly recovered from blood stream (53.0%, P = 0.014), while SCCmec-IV/V strains were predominately isolated from skin and soft tissue and abscess (55.0%, P < 0.001). The PVL gene was detected in 10.0% of SCCmec-IV/V isolates in contrast to 0.3% in SCCmec-I/II/III (chi(2) = 25.164, P < 0.001). SCCmec-I/II/III MRSA strains were more resistant to clindamycin (chi(2) = 5.078, P = 0.024), amoxicillin-clavulanate (chi(2) = 84.912, P < 0.001), erythromycin (chi(2) = 4.651, P = 0.031), gentamicin (chi(2) = 24.869, P < 0.001), and rifampin (chi(2) = 18.878, P < 0.001) than SCCmec-IV/V MRSA strains. This data indicates that SCCmec-III MRSA strains that do not carry the PVL gene are the predominant MRSA strains in our hospital setting in Ankara, capital of Turkey and that SCCmec-I/II/III MRSA strains may cause serious infections in surgical departments especially those with intensive care units. SN - 1572-9699 UR - https://www.unboundmedicine.com/medline/citation/18752036/Staphylococcal_cassette_chromosome_mec__SCCmec__characterization_and_panton_valentine_leukocidin_gene_occurrence_for_methicillin_resistant_Staphylococcus_aureus_in_Turkey_from_2003_to_2006_ L2 - https://doi.org/10.1007/s10482-008-9278-3 DB - PRIME DP - Unbound Medicine ER -