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In-capillary derivatization and capillary electrophoresis separation of amino acid neurotransmitters from brain microdialysis samples.
J Chromatogr A. 2008 Sep 26; 1205(1-2):144-9.JC

Abstract

A new in-capillary derivatization method with naphtalene-2,3-dicarboxyaldehyde (NDA)/CN(-) has been developed for capillary electrophoresis with laser-induced fluorescence detection of brain microdialysate amino acids. Samples are sandwiched between two plugs of reagent mixture at the capillary inlet and subsequently separated. Highest derivatization yields are obtained by using a reagent to sample plug length ratio equal to 4, performing a first electrophoretic mixing followed by a zero potential amplification step before applying the separation voltage and using a NaCN to NDA concentration ratio equal to 1. This new single-step methodology allows the analysis of amino acid neurotransmitters in rat brain microdialysis samples.

Authors+Show Affiliations

Université de Lyon, F-69008 Lyon, France. luc.denoroy@recherche.univ-lyon1.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

18752800

Citation

Denoroy, Luc, et al. "In-capillary Derivatization and Capillary Electrophoresis Separation of Amino Acid Neurotransmitters From Brain Microdialysis Samples." Journal of Chromatography. A, vol. 1205, no. 1-2, 2008, pp. 144-9.
Denoroy L, Parrot S, Renaud L, et al. In-capillary derivatization and capillary electrophoresis separation of amino acid neurotransmitters from brain microdialysis samples. J Chromatogr A. 2008;1205(1-2):144-9.
Denoroy, L., Parrot, S., Renaud, L., Renaud, B., & Zimmer, L. (2008). In-capillary derivatization and capillary electrophoresis separation of amino acid neurotransmitters from brain microdialysis samples. Journal of Chromatography. A, 1205(1-2), 144-9. https://doi.org/10.1016/j.chroma.2008.07.043
Denoroy L, et al. In-capillary Derivatization and Capillary Electrophoresis Separation of Amino Acid Neurotransmitters From Brain Microdialysis Samples. J Chromatogr A. 2008 Sep 26;1205(1-2):144-9. PubMed PMID: 18752800.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In-capillary derivatization and capillary electrophoresis separation of amino acid neurotransmitters from brain microdialysis samples. AU - Denoroy,Luc, AU - Parrot,Sandrine, AU - Renaud,Louis, AU - Renaud,Bernard, AU - Zimmer,Luc, Y1 - 2008/07/19/ PY - 2008/05/09/received PY - 2008/07/10/revised PY - 2008/07/16/accepted PY - 2008/8/30/pubmed PY - 2008/12/17/medline PY - 2008/8/30/entrez SP - 144 EP - 9 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1205 IS - 1-2 N2 - A new in-capillary derivatization method with naphtalene-2,3-dicarboxyaldehyde (NDA)/CN(-) has been developed for capillary electrophoresis with laser-induced fluorescence detection of brain microdialysate amino acids. Samples are sandwiched between two plugs of reagent mixture at the capillary inlet and subsequently separated. Highest derivatization yields are obtained by using a reagent to sample plug length ratio equal to 4, performing a first electrophoretic mixing followed by a zero potential amplification step before applying the separation voltage and using a NaCN to NDA concentration ratio equal to 1. This new single-step methodology allows the analysis of amino acid neurotransmitters in rat brain microdialysis samples. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/18752800/In_capillary_derivatization_and_capillary_electrophoresis_separation_of_amino_acid_neurotransmitters_from_brain_microdialysis_samples_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(08)01222-3 DB - PRIME DP - Unbound Medicine ER -