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[Expression of galectin-3 in liver metastasis of colon cancer and the inhibitory effect of modified citrus pectin].
Nan Fang Yi Ke Da Xue Xue Bao. 2008 Aug; 28(8):1358-61.NF

Abstract

OBJECTIVE

To observe the expression of galectin-3 in the liver metastasis of colon cancer in mice and the inhibitory effect of modified citrus pectin (MCP) on galectin-3 expression.

METHODS

Seventy-five Balb/c mice were randomized into 5 groups, namely the negative control, positive control, low-concentration MCP, moderate-concentration MCP and high-concentration MCP groups. CT26 colon cancer cells were injected into the subcapsule of the mouse spleen to establish liver metastasis models of colon cancer, but the mice in the negative control group received no tumor cell injection. MCP was added into the drinking water of the mice at the concentrations of 0, 1.0%, 2.5% and 5.0% (m/V). The liver metastasis was observed 3 weeks after tumor cell inoculation. Enzyme-linked immunosorbent assay was performed to determine the serum galectin-3 level. A tissue microarray of the liver metastasis was prepared for immunohistochemical detection of galectin-3 expression in the liver metastasis.

RESULTS

In the positive control, low-, moderate- and high-concentration MCP groups, the rates of liver metastasis were 100%, 80%, 73.3% and 60%, respectively. The number of liver metastases in high-concentration MCP group was significantly smaller than that in the positive control group (P<0.05). In the 4 groups with tumor cell inoculation, the median volume of the primary lesions in the spleen was 1.51, 0.93, 0.77 and 0.70 cm(3), respectively, which were significantly smaller in the moderate- and high-concentration MCP groups than in the positive control group (P<0.05). The serum galectin-3 level in the positive control group and MCP-treated groups were significantly higher than that in the negative control group (P<0.01), but similar between the positive control group and the MCP-treated groups (P>0.05). In the positive control and the MCP-treated groups, the expression of galectin-3 in the liver metastases showed no significant differences (P>0.05).

CONCLUSION

The expression of galetin-3 is significantly increased in the liver metastasis of colon cancer, and MCP can effectively inhibit the liver metastasis.

Authors+Show Affiliations

Department of Abdominal Surgery, Affiliated Tumor Hospital of Guangzhou Medical College, Guangzhou, China. gzdlhy@yahoo.com.cnNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

18753060

Citation

Huang, Zhi-liang, and Hai-ying Liu. "[Expression of Galectin-3 in Liver Metastasis of Colon Cancer and the Inhibitory Effect of Modified Citrus Pectin]." Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, vol. 28, no. 8, 2008, pp. 1358-61.
Huang ZL, Liu HY. [Expression of galectin-3 in liver metastasis of colon cancer and the inhibitory effect of modified citrus pectin]. Nan Fang Yi Ke Da Xue Xue Bao. 2008;28(8):1358-61.
Huang, Z. L., & Liu, H. Y. (2008). [Expression of galectin-3 in liver metastasis of colon cancer and the inhibitory effect of modified citrus pectin]. Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, 28(8), 1358-61.
Huang ZL, Liu HY. [Expression of Galectin-3 in Liver Metastasis of Colon Cancer and the Inhibitory Effect of Modified Citrus Pectin]. Nan Fang Yi Ke Da Xue Xue Bao. 2008;28(8):1358-61. PubMed PMID: 18753060.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Expression of galectin-3 in liver metastasis of colon cancer and the inhibitory effect of modified citrus pectin]. AU - Huang,Zhi-liang, AU - Liu,Hai-ying, PY - 2008/8/30/pubmed PY - 2009/4/1/medline PY - 2008/8/30/entrez SP - 1358 EP - 61 JF - Nan fang yi ke da xue xue bao = Journal of Southern Medical University JO - Nan Fang Yi Ke Da Xue Xue Bao VL - 28 IS - 8 N2 - OBJECTIVE: To observe the expression of galectin-3 in the liver metastasis of colon cancer in mice and the inhibitory effect of modified citrus pectin (MCP) on galectin-3 expression. METHODS: Seventy-five Balb/c mice were randomized into 5 groups, namely the negative control, positive control, low-concentration MCP, moderate-concentration MCP and high-concentration MCP groups. CT26 colon cancer cells were injected into the subcapsule of the mouse spleen to establish liver metastasis models of colon cancer, but the mice in the negative control group received no tumor cell injection. MCP was added into the drinking water of the mice at the concentrations of 0, 1.0%, 2.5% and 5.0% (m/V). The liver metastasis was observed 3 weeks after tumor cell inoculation. Enzyme-linked immunosorbent assay was performed to determine the serum galectin-3 level. A tissue microarray of the liver metastasis was prepared for immunohistochemical detection of galectin-3 expression in the liver metastasis. RESULTS: In the positive control, low-, moderate- and high-concentration MCP groups, the rates of liver metastasis were 100%, 80%, 73.3% and 60%, respectively. The number of liver metastases in high-concentration MCP group was significantly smaller than that in the positive control group (P<0.05). In the 4 groups with tumor cell inoculation, the median volume of the primary lesions in the spleen was 1.51, 0.93, 0.77 and 0.70 cm(3), respectively, which were significantly smaller in the moderate- and high-concentration MCP groups than in the positive control group (P<0.05). The serum galectin-3 level in the positive control group and MCP-treated groups were significantly higher than that in the negative control group (P<0.01), but similar between the positive control group and the MCP-treated groups (P>0.05). In the positive control and the MCP-treated groups, the expression of galectin-3 in the liver metastases showed no significant differences (P>0.05). CONCLUSION: The expression of galetin-3 is significantly increased in the liver metastasis of colon cancer, and MCP can effectively inhibit the liver metastasis. SN - 1673-4254 UR - https://www.unboundmedicine.com/medline/citation/18753060/[Expression_of_galectin_3_in_liver_metastasis_of_colon_cancer_and_the_inhibitory_effect_of_modified_citrus_pectin]_ L2 - https://medlineplus.gov/livercancer.html DB - PRIME DP - Unbound Medicine ER -