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Estradiol replacement enhances sleep deprivation-induced c-Fos immunoreactivity in forebrain arousal regions of ovariectomized rats.

Abstract

To understand how female sex hormones influence homeostatic mechanisms of sleep, we studied the effects of estradiol (E(2)) replacement on c-Fos immunoreactivity in sleep/wake-regulatory brain areas after sleep deprivation (SD) in ovariectomized rats. Adult rats were ovariectomized and implanted subcutaneously with capsules containing 17beta-E(2) (10.5 microg; to mimic diestrous E(2) levels) or oil. After 2 wk, animals with E(2) capsules received a single subcutaneous injection of 17beta-E(2) (10 microg/kg; to achieve proestrous E(2) levels) or oil; control animals with oil capsules received an oil injection. Twenty-four hours later, animals were either left undisturbed or sleep deprived by "gentle handling" for 6 h during the early light phase, and killed. E(2) treatment increased serum E(2) levels and uterus weights dose dependently, while attenuating body weight gain. Regardless of hormonal conditions, SD increased c-Fos immunoreactivity in all four arousal-promoting areas and four limbic and neuroendocrine nuclei studied, whereas it decreased c-Fos labeling in the sleep-promoting ventrolateral preoptic nucleus (VLPO). Low and high E(2) treatments enhanced the SD-induced c-Fos immunoreactivity in the laterodorsal subnucleus of the bed nucleus of stria terminalis and the tuberomammillary nucleus, and in orexin-containing hypothalamic neurons, with no effect on the basal forebrain and locus coeruleus. The high E(2) treatment decreased c-Fos labeling in the VLPO under nondeprived conditions. These results indicate that E(2) replacement modulates SD-induced or spontaneous c-Fos expression in sleep/wake-regulatory and limbic forebrain nuclei. These modulatory effects of E(2) replacement on neuronal activity may be, in part, responsible for E(2)'s influence on sleep/wake behavior.

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  • Authors+Show Affiliations

    ,

    Dept. of Anatomy and Neurobiology, Dalhousie Univ., Tupper Medical Bldg., Rm. 13MN, 5850 College St., Halifax, NS B3H 1X5 Canada.

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    Source

    MeSH

    Animals
    Body Weight
    Estradiol
    Estrogen Replacement Therapy
    Female
    Hypothalamic Area, Lateral
    Intracellular Signaling Peptides and Proteins
    Neurons
    Neuropeptides
    Orexins
    Organ Size
    Ovariectomy
    Preoptic Area
    Prosencephalon
    Proto-Oncogene Proteins c-fos
    Rats
    Rats, Wistar
    Septal Nuclei
    Sleep
    Sleep Deprivation
    Uterus

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    18753261

    Citation

    Deurveilher, S, et al. "Estradiol Replacement Enhances Sleep Deprivation-induced c-Fos Immunoreactivity in Forebrain Arousal Regions of Ovariectomized Rats." American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, vol. 295, no. 4, 2008, pp. R1328-40.
    Deurveilher S, Cumyn EM, Peers T, et al. Estradiol replacement enhances sleep deprivation-induced c-Fos immunoreactivity in forebrain arousal regions of ovariectomized rats. Am J Physiol Regul Integr Comp Physiol. 2008;295(4):R1328-40.
    Deurveilher, S., Cumyn, E. M., Peers, T., Rusak, B., & Semba, K. (2008). Estradiol replacement enhances sleep deprivation-induced c-Fos immunoreactivity in forebrain arousal regions of ovariectomized rats. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 295(4), pp. R1328-40. doi:10.1152/ajpregu.90576.2008.
    Deurveilher S, et al. Estradiol Replacement Enhances Sleep Deprivation-induced c-Fos Immunoreactivity in Forebrain Arousal Regions of Ovariectomized Rats. Am J Physiol Regul Integr Comp Physiol. 2008;295(4):R1328-40. PubMed PMID: 18753261.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Estradiol replacement enhances sleep deprivation-induced c-Fos immunoreactivity in forebrain arousal regions of ovariectomized rats. AU - Deurveilher,S, AU - Cumyn,E M, AU - Peers,T, AU - Rusak,B, AU - Semba,K, Y1 - 2008/08/27/ PY - 2008/8/30/pubmed PY - 2008/12/17/medline PY - 2008/8/30/entrez SP - R1328 EP - 40 JF - American journal of physiology. Regulatory, integrative and comparative physiology JO - Am. J. Physiol. Regul. Integr. Comp. Physiol. VL - 295 IS - 4 N2 - To understand how female sex hormones influence homeostatic mechanisms of sleep, we studied the effects of estradiol (E(2)) replacement on c-Fos immunoreactivity in sleep/wake-regulatory brain areas after sleep deprivation (SD) in ovariectomized rats. Adult rats were ovariectomized and implanted subcutaneously with capsules containing 17beta-E(2) (10.5 microg; to mimic diestrous E(2) levels) or oil. After 2 wk, animals with E(2) capsules received a single subcutaneous injection of 17beta-E(2) (10 microg/kg; to achieve proestrous E(2) levels) or oil; control animals with oil capsules received an oil injection. Twenty-four hours later, animals were either left undisturbed or sleep deprived by "gentle handling" for 6 h during the early light phase, and killed. E(2) treatment increased serum E(2) levels and uterus weights dose dependently, while attenuating body weight gain. Regardless of hormonal conditions, SD increased c-Fos immunoreactivity in all four arousal-promoting areas and four limbic and neuroendocrine nuclei studied, whereas it decreased c-Fos labeling in the sleep-promoting ventrolateral preoptic nucleus (VLPO). Low and high E(2) treatments enhanced the SD-induced c-Fos immunoreactivity in the laterodorsal subnucleus of the bed nucleus of stria terminalis and the tuberomammillary nucleus, and in orexin-containing hypothalamic neurons, with no effect on the basal forebrain and locus coeruleus. The high E(2) treatment decreased c-Fos labeling in the VLPO under nondeprived conditions. These results indicate that E(2) replacement modulates SD-induced or spontaneous c-Fos expression in sleep/wake-regulatory and limbic forebrain nuclei. These modulatory effects of E(2) replacement on neuronal activity may be, in part, responsible for E(2)'s influence on sleep/wake behavior. SN - 0363-6119 UR - https://www.unboundmedicine.com/medline/citation/18753261/Estradiol_replacement_enhances_sleep_deprivation_induced_c_Fos_immunoreactivity_in_forebrain_arousal_regions_of_ovariectomized_rats_ L2 - http://www.physiology.org/doi/full/10.1152/ajpregu.90576.2008?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -