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Quantification of DNA in plasma by an automated real-time PCR assay (cytomegalovirus PCR kit) for surveillance of active cytomegalovirus infection and guidance of preemptive therapy for allogeneic hematopoietic stem cell transplant recipients.
J Clin Microbiol. 2008 Oct; 46(10):3311-8.JC

Abstract

The performance of a plasma real-time PCR (cytomegalovirus [CMV] PCR kit; Abbott Diagnostics) was compared with that of the antigenemia assay for the surveillance of active CMV infection in 42 allogeneic hematopoietic stem cell transplantation (Allo-SCT) recipients. A total of 1,156 samples were analyzed by the two assays. Concordance between the two assays was 82.2%. Plasma DNA levels correlated with the number of pp65-positive cells, particularly prior to the initiation of preemptive therapy. Fifty-seven episodes of active CMV infection were detected in 37 patients: 18 were defined solely by the PCR assay and four were defined on the basis of the antigenemia assay. Either a cutoff of 288 CMV DNA copies/ml or a 2.42-log(10) increase of DNAemia levels between two consecutive PCR positive samples was an optimal value to discriminate between patients requiring preemptive therapy and those not requiring therapy on the basis of the antigenemia results. The real-time PCR assay allowed an earlier diagnosis of active CMV infection and was a more reliable marker of successful clearance of CMV from the blood. Analysis of the kinetics of DNAemia levels at a median of 7 days posttreatment allowed the prediction of the response to CMV therapy. Two patients developed CMV colitis. The PCR assay tested positive both before the onset of symptoms and during the disease period. The plasma real-time PCR from Abbott is more suitable than the antigenemia assay for monitoring active CMV infection in Allo-SCT recipients and may be used for guiding preemptive therapy in this clinical setting.

Authors+Show Affiliations

Microbiology Service, Hospital Clínico Universitario, Valencia, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18753357

Citation

Gimeno, Concepción, et al. "Quantification of DNA in Plasma By an Automated Real-time PCR Assay (cytomegalovirus PCR Kit) for Surveillance of Active Cytomegalovirus Infection and Guidance of Preemptive Therapy for Allogeneic Hematopoietic Stem Cell Transplant Recipients." Journal of Clinical Microbiology, vol. 46, no. 10, 2008, pp. 3311-8.
Gimeno C, Solano C, Latorre JC, et al. Quantification of DNA in plasma by an automated real-time PCR assay (cytomegalovirus PCR kit) for surveillance of active cytomegalovirus infection and guidance of preemptive therapy for allogeneic hematopoietic stem cell transplant recipients. J Clin Microbiol. 2008;46(10):3311-8.
Gimeno, C., Solano, C., Latorre, J. C., Hernández-Boluda, J. C., Clari, M. A., Remigia, M. J., Furió, S., Calabuig, M., Tormo, N., & Navarro, D. (2008). Quantification of DNA in plasma by an automated real-time PCR assay (cytomegalovirus PCR kit) for surveillance of active cytomegalovirus infection and guidance of preemptive therapy for allogeneic hematopoietic stem cell transplant recipients. Journal of Clinical Microbiology, 46(10), 3311-8. https://doi.org/10.1128/JCM.00797-08
Gimeno C, et al. Quantification of DNA in Plasma By an Automated Real-time PCR Assay (cytomegalovirus PCR Kit) for Surveillance of Active Cytomegalovirus Infection and Guidance of Preemptive Therapy for Allogeneic Hematopoietic Stem Cell Transplant Recipients. J Clin Microbiol. 2008;46(10):3311-8. PubMed PMID: 18753357.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantification of DNA in plasma by an automated real-time PCR assay (cytomegalovirus PCR kit) for surveillance of active cytomegalovirus infection and guidance of preemptive therapy for allogeneic hematopoietic stem cell transplant recipients. AU - Gimeno,Concepción, AU - Solano,Carlos, AU - Latorre,José C, AU - Hernández-Boluda,Juan C, AU - Clari,María A, AU - Remigia,María J, AU - Furió,Santiago, AU - Calabuig,Marisa, AU - Tormo,Nuria, AU - Navarro,David, Y1 - 2008/08/27/ PY - 2008/8/30/pubmed PY - 2008/10/28/medline PY - 2008/8/30/entrez SP - 3311 EP - 8 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 46 IS - 10 N2 - The performance of a plasma real-time PCR (cytomegalovirus [CMV] PCR kit; Abbott Diagnostics) was compared with that of the antigenemia assay for the surveillance of active CMV infection in 42 allogeneic hematopoietic stem cell transplantation (Allo-SCT) recipients. A total of 1,156 samples were analyzed by the two assays. Concordance between the two assays was 82.2%. Plasma DNA levels correlated with the number of pp65-positive cells, particularly prior to the initiation of preemptive therapy. Fifty-seven episodes of active CMV infection were detected in 37 patients: 18 were defined solely by the PCR assay and four were defined on the basis of the antigenemia assay. Either a cutoff of 288 CMV DNA copies/ml or a 2.42-log(10) increase of DNAemia levels between two consecutive PCR positive samples was an optimal value to discriminate between patients requiring preemptive therapy and those not requiring therapy on the basis of the antigenemia results. The real-time PCR assay allowed an earlier diagnosis of active CMV infection and was a more reliable marker of successful clearance of CMV from the blood. Analysis of the kinetics of DNAemia levels at a median of 7 days posttreatment allowed the prediction of the response to CMV therapy. Two patients developed CMV colitis. The PCR assay tested positive both before the onset of symptoms and during the disease period. The plasma real-time PCR from Abbott is more suitable than the antigenemia assay for monitoring active CMV infection in Allo-SCT recipients and may be used for guiding preemptive therapy in this clinical setting. SN - 1098-660X UR - https://www.unboundmedicine.com/medline/citation/18753357/Quantification_of_DNA_in_plasma_by_an_automated_real_time_PCR_assay__cytomegalovirus_PCR_kit__for_surveillance_of_active_cytomegalovirus_infection_and_guidance_of_preemptive_therapy_for_allogeneic_hematopoietic_stem_cell_transplant_recipients_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=18753357 DB - PRIME DP - Unbound Medicine ER -