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Effects of ascorbic acid, phytic acid and tannic acid on iron bioavailability from reconstituted ferritin measured by an in vitro digestion-Caco-2 cell model.
Br J Nutr. 2009 Apr; 101(7):972-81.BJ

Abstract

The effects of ascorbic acid (AA), phytate and tannic acid (TA) on Fe bioavailability from Fe supplied as reconstituted ferritin were compared with FeSO4 using an in vitro digestion-Caco-2 cell model. Horse spleen apoferritin was chemically reconstituted into an animal-type ferritin (HSF) and a plant-type ferritin (P-HSF) according to the typical ratios of Fe:P found in these molecules. In the presence of AA (Fe:AA molar ratio of 1:20), significantly more Fe was absorbed from FeSO4 (about 303 %), HSF (about 454 %) and P-HSF (about 371 %) when compared with ferrous sulfate or ferritin without AA. Phytic acid (PA; Fe:PA molar ratio of 1:20) significantly reduced Fe bioavailability from FeSO4 (about 86 %), HSF (about 82 %) and P-HSF (about 93 %) relative to FeSO4 and the ferritin controls. Treatment with TA (Fe:TA molar ratio of 1:1) significantly decreased Fe bioavailability (about 97 %) from both FeSO4 and the ferritin samples. AA was able to partially reverse the negative effect of PA (Fe:PA:AA molar ratio of 1:20:20) on Fe bioavailability but did not reverse the inhibiting effect of TA (Fe:TA:AA molar ratio of 1:1:20) on Fe bioavailability from ferritin and FeSO4. Overall, there were no significant differences in bioavailable Fe between P-HSF, HSF or FeSO4. Furthermore, the addition of AA (a known promoter) or the inhibitors, PA and TA, or both, did not result in significant differences in bioavailable Fe from ferritin relative to FeSO4. The results suggest that Fe in the reconstituted ferritin molecule is easily released during in vitro digestion and interacts with known promoters and inhibitors.

Authors+Show Affiliations

USDA-ARS, The Robert W. Holley Center for Health and Agriculture, Tower Road, Ithaca, New York 14853, USA. jinf@appstate.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

18755051

Citation

Jin, Fuxia, et al. "Effects of Ascorbic Acid, Phytic Acid and Tannic Acid On Iron Bioavailability From Reconstituted Ferritin Measured By an in Vitro digestion-Caco-2 Cell Model." The British Journal of Nutrition, vol. 101, no. 7, 2009, pp. 972-81.
Jin F, Frohman C, Thannhauser TW, et al. Effects of ascorbic acid, phytic acid and tannic acid on iron bioavailability from reconstituted ferritin measured by an in vitro digestion-Caco-2 cell model. Br J Nutr. 2009;101(7):972-81.
Jin, F., Frohman, C., Thannhauser, T. W., Welch, R. M., & Glahn, R. P. (2009). Effects of ascorbic acid, phytic acid and tannic acid on iron bioavailability from reconstituted ferritin measured by an in vitro digestion-Caco-2 cell model. The British Journal of Nutrition, 101(7), 972-81. https://doi.org/10.1017/S0007114508055621
Jin F, et al. Effects of Ascorbic Acid, Phytic Acid and Tannic Acid On Iron Bioavailability From Reconstituted Ferritin Measured By an in Vitro digestion-Caco-2 Cell Model. Br J Nutr. 2009;101(7):972-81. PubMed PMID: 18755051.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of ascorbic acid, phytic acid and tannic acid on iron bioavailability from reconstituted ferritin measured by an in vitro digestion-Caco-2 cell model. AU - Jin,Fuxia, AU - Frohman,Charles, AU - Thannhauser,Theodore W, AU - Welch,Ross M, AU - Glahn,Raymond P, Y1 - 2008/08/28/ PY - 2008/8/30/pubmed PY - 2009/6/20/medline PY - 2008/8/30/entrez SP - 972 EP - 81 JF - The British journal of nutrition JO - Br. J. Nutr. VL - 101 IS - 7 N2 - The effects of ascorbic acid (AA), phytate and tannic acid (TA) on Fe bioavailability from Fe supplied as reconstituted ferritin were compared with FeSO4 using an in vitro digestion-Caco-2 cell model. Horse spleen apoferritin was chemically reconstituted into an animal-type ferritin (HSF) and a plant-type ferritin (P-HSF) according to the typical ratios of Fe:P found in these molecules. In the presence of AA (Fe:AA molar ratio of 1:20), significantly more Fe was absorbed from FeSO4 (about 303 %), HSF (about 454 %) and P-HSF (about 371 %) when compared with ferrous sulfate or ferritin without AA. Phytic acid (PA; Fe:PA molar ratio of 1:20) significantly reduced Fe bioavailability from FeSO4 (about 86 %), HSF (about 82 %) and P-HSF (about 93 %) relative to FeSO4 and the ferritin controls. Treatment with TA (Fe:TA molar ratio of 1:1) significantly decreased Fe bioavailability (about 97 %) from both FeSO4 and the ferritin samples. AA was able to partially reverse the negative effect of PA (Fe:PA:AA molar ratio of 1:20:20) on Fe bioavailability but did not reverse the inhibiting effect of TA (Fe:TA:AA molar ratio of 1:1:20) on Fe bioavailability from ferritin and FeSO4. Overall, there were no significant differences in bioavailable Fe between P-HSF, HSF or FeSO4. Furthermore, the addition of AA (a known promoter) or the inhibitors, PA and TA, or both, did not result in significant differences in bioavailable Fe from ferritin relative to FeSO4. The results suggest that Fe in the reconstituted ferritin molecule is easily released during in vitro digestion and interacts with known promoters and inhibitors. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/18755051/Effects_of_ascorbic_acid_phytic_acid_and_tannic_acid_on_iron_bioavailability_from_reconstituted_ferritin_measured_by_an_in_vitro_digestion_Caco_2_cell_model_ L2 - https://www.cambridge.org/core/product/identifier/S0007114508055621/type/journal_article DB - PRIME DP - Unbound Medicine ER -