Tags

Type your tag names separated by a space and hit enter

Au-nanoparticles as an electrochemical sensing platform for aptamer-thrombin interaction.
Biosens Bioelectron. 2008 Dec 01; 24(4):831-6.BB

Abstract

A novel electrochemical method for the detection of bioaffinity interactions based on a gold-nanoparticles sensing platform and on the usage of stripping voltammetry technique was developed. The oxidation of gold surface (resulted in gold oxide formation) upon polarization served as a basis for analytical response. As a model, thrombin-thrombin binding aptamer couple was chosen. The aptamer was immobilized on a screen-printed electrode modified with gold-nanoparticles by avidin-biotin technology. Cathodic peak area was found proportional to thrombin quantity specifically adsorbed onto electrode surface. Sigmoid calibration curve as is typical for immunoassay was obtained, with thrombin detection limit of 10(-9)M. Linear range corresponds from 10(-8) to 10(-5)M thrombin concentration or 2 x 10(-14) to 2 x 10(-11)mol/electrode (R=0.996). Binding of thrombin to an aptamer has also been detected using the ferricyanide/ferrocyanide redox couple as electrochemical indicator.

Links

Publisher Full Text

Authors+Show Affiliations

Suprun E
Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Pogodinskaya Street 10, Moscow 119121, Russia.
Shumyantseva V
No affiliation info available
Bulko T
No affiliation info available
Rachmetova S
No affiliation info available
Rad'ko S
No affiliation info available
Bodoev N
No affiliation info available
Archakov A
No affiliation info available

MeSH

Aptamers, PeptideBiosensing TechniquesElectrochemistryEquipment DesignEquipment Failure AnalysisGoldImmunoassayNanoparticlesParticle SizeProtein Interaction MappingReproducibility of ResultsSensitivity and SpecificityThrombin

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18755579

Citation

Suprun, Elena, et al. "Au-nanoparticles as an Electrochemical Sensing Platform for Aptamer-thrombin Interaction." Biosensors & Bioelectronics, vol. 24, no. 4, 2008, pp. 831-6.
Suprun E, Shumyantseva V, Bulko T, et al. Au-nanoparticles as an electrochemical sensing platform for aptamer-thrombin interaction. Biosens Bioelectron. 2008;24(4):831-6.
Suprun, E., Shumyantseva, V., Bulko, T., Rachmetova, S., Rad'ko, S., Bodoev, N., & Archakov, A. (2008). Au-nanoparticles as an electrochemical sensing platform for aptamer-thrombin interaction. Biosensors & Bioelectronics, 24(4), 831-6. https://doi.org/10.1016/j.bios.2008.07.008
Suprun E, et al. Au-nanoparticles as an Electrochemical Sensing Platform for Aptamer-thrombin Interaction. Biosens Bioelectron. 2008 Dec 1;24(4):831-6. PubMed PMID: 18755579.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Au-nanoparticles as an electrochemical sensing platform for aptamer-thrombin interaction. AU - Suprun,Elena, AU - Shumyantseva,Victoria, AU - Bulko,Tatiana, AU - Rachmetova,Svetlana, AU - Rad'ko,Sergei, AU - Bodoev,Nikolay, AU - Archakov,Alexander, Y1 - 2008/07/15/ PY - 2008/03/20/received PY - 2008/07/03/revised PY - 2008/07/04/accepted PY - 2008/8/30/pubmed PY - 2009/2/13/medline PY - 2008/8/30/entrez SP - 831 EP - 6 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 24 IS - 4 N2 - A novel electrochemical method for the detection of bioaffinity interactions based on a gold-nanoparticles sensing platform and on the usage of stripping voltammetry technique was developed. The oxidation of gold surface (resulted in gold oxide formation) upon polarization served as a basis for analytical response. As a model, thrombin-thrombin binding aptamer couple was chosen. The aptamer was immobilized on a screen-printed electrode modified with gold-nanoparticles by avidin-biotin technology. Cathodic peak area was found proportional to thrombin quantity specifically adsorbed onto electrode surface. Sigmoid calibration curve as is typical for immunoassay was obtained, with thrombin detection limit of 10(-9)M. Linear range corresponds from 10(-8) to 10(-5)M thrombin concentration or 2 x 10(-14) to 2 x 10(-11)mol/electrode (R=0.996). Binding of thrombin to an aptamer has also been detected using the ferricyanide/ferrocyanide redox couple as electrochemical indicator. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/18755579/Au_nanoparticles_as_an_electrochemical_sensing_platform_for_aptamer_thrombin_interaction_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(08)00354-0 DB - PRIME DP - Unbound Medicine ER -