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The interaction of gabapentin and N6-(2-phenylisopropyl)-adenosine R-(-)isomer (R-PIA) on mechanical allodynia in rats with a spinal nerve ligation.
J Korean Med Sci. 2008 Aug; 23(4):678-84.JK

Abstract

We examined the antiallodynic interaction between gabapentin and adenosine A1 receptor agonist, N(6)-(2-phenylisopropyl)-adenosine R-(-)isomer (R-PIA), in a rat model of nerve ligation injury. Rats were prepared with ligation of left L5-6 spinal nerves and intrathecal catheter implantation for drug administration. Mechanical allodynia was measured by applying von Frey filaments. Gabapentin and R-PIA were administered to obtain the dose-response curve and the 50% effective dose (ED(50)). Fractions of ED(50)s were administered concurrently to establish the ED(50) of the drug combination. The drug interaction between gabapentin and R-PIA was analyzed using the isobolographic method. Adenosine A1 receptor antagonist was administered intrathecally to examine the reversal of the antiallodynic effect. Locomotor function changes were evaluated by rotarod testing. Intrathecal gabapentin and R-PIA and their combination produced a dose-dependent antagonism against mechanical allodynia without severe side effects. Intrathecal gabapentin synergistically enhanced the antiallodynic effect of R-PIA when coadministered. There were no significant changes in rotarod performance time, except gabapentin 300 microg. In the combination group, the maximal antiallodynic effect was reversed by A1 adenosine receptor antagonist. These results suggest that activation of adenosine A1 receptors at the spinal level is required for the synergistic interaction on the mechanical allodynia.

Authors+Show Affiliations

Department of Anesthesiology and Pain Medicine, University of Ulsan, College of Medicine, Seoul Asan Medical Center, Songpa-gu, Seoul, Korea. jongyeon_park@amc.seoul.krNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18756057

Citation

Park, Jong Yeon, and In Gu Jun. "The Interaction of Gabapentin and N6-(2-phenylisopropyl)-adenosine R-(-)isomer (R-PIA) On Mechanical Allodynia in Rats With a Spinal Nerve Ligation." Journal of Korean Medical Science, vol. 23, no. 4, 2008, pp. 678-84.
Park JY, Jun IG. The interaction of gabapentin and N6-(2-phenylisopropyl)-adenosine R-(-)isomer (R-PIA) on mechanical allodynia in rats with a spinal nerve ligation. J Korean Med Sci. 2008;23(4):678-84.
Park, J. Y., & Jun, I. G. (2008). The interaction of gabapentin and N6-(2-phenylisopropyl)-adenosine R-(-)isomer (R-PIA) on mechanical allodynia in rats with a spinal nerve ligation. Journal of Korean Medical Science, 23(4), 678-84. https://doi.org/10.3346/jkms.2008.23.4.678
Park JY, Jun IG. The Interaction of Gabapentin and N6-(2-phenylisopropyl)-adenosine R-(-)isomer (R-PIA) On Mechanical Allodynia in Rats With a Spinal Nerve Ligation. J Korean Med Sci. 2008;23(4):678-84. PubMed PMID: 18756057.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The interaction of gabapentin and N6-(2-phenylisopropyl)-adenosine R-(-)isomer (R-PIA) on mechanical allodynia in rats with a spinal nerve ligation. AU - Park,Jong Yeon, AU - Jun,In Gu, PY - 2008/8/30/pubmed PY - 2008/10/8/medline PY - 2008/8/30/entrez SP - 678 EP - 84 JF - Journal of Korean medical science JO - J Korean Med Sci VL - 23 IS - 4 N2 - We examined the antiallodynic interaction between gabapentin and adenosine A1 receptor agonist, N(6)-(2-phenylisopropyl)-adenosine R-(-)isomer (R-PIA), in a rat model of nerve ligation injury. Rats were prepared with ligation of left L5-6 spinal nerves and intrathecal catheter implantation for drug administration. Mechanical allodynia was measured by applying von Frey filaments. Gabapentin and R-PIA were administered to obtain the dose-response curve and the 50% effective dose (ED(50)). Fractions of ED(50)s were administered concurrently to establish the ED(50) of the drug combination. The drug interaction between gabapentin and R-PIA was analyzed using the isobolographic method. Adenosine A1 receptor antagonist was administered intrathecally to examine the reversal of the antiallodynic effect. Locomotor function changes were evaluated by rotarod testing. Intrathecal gabapentin and R-PIA and their combination produced a dose-dependent antagonism against mechanical allodynia without severe side effects. Intrathecal gabapentin synergistically enhanced the antiallodynic effect of R-PIA when coadministered. There were no significant changes in rotarod performance time, except gabapentin 300 microg. In the combination group, the maximal antiallodynic effect was reversed by A1 adenosine receptor antagonist. These results suggest that activation of adenosine A1 receptors at the spinal level is required for the synergistic interaction on the mechanical allodynia. SN - 1011-8934 UR - https://www.unboundmedicine.com/medline/citation/18756057/The_interaction_of_gabapentin_and_N6__2_phenylisopropyl__adenosine_R____isomer__R_PIA__on_mechanical_allodynia_in_rats_with_a_spinal_nerve_ligation_ L2 - https://jkms.org/DOIx.php?id=10.3346/jkms.2008.23.4.678 DB - PRIME DP - Unbound Medicine ER -